Results 11 to 20 of about 13,061,353 (246)
Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis [PDF]
Frontiers in Genetics, 2020 Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis.
Yao Lv +9 more
doaj +3 more sources
Integrative analysis of 111 reference human epigenomes
Nature, 2015 The reference human genome sequence set the stage for studies of genetic variation and its association with human disease, but epigenomic studies lack a similar reference. To address this need, the NIH Roadmap Epigenomics Consortium generated the largest
R. Consortium +95 more
semanticscholar +3 more sources
Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]
BMC Genomics, 2019 Abstract Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels.
Karen Cristine Gonçalves Dos Santos +2 more
openaire +4 more sources
Reference genes in real-time PCR [PDF]
Journal of Applied Genetics, 2013 This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Kozera, Bartłomiej, Rapacz, Marcin
openaire +2 more sources
RefPrimeCouch—a reference gene primer CouchApp [PDF]
Database, 2013 To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha +3 more
openaire +3 more sources
Reference genes for measuring mRNA expression [PDF]
Theory in Biosciences, 2012 The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Dundas, J, Ling, M
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Identification of stable senescence‐associated reference genes [PDF]
Aging Cell, 2019 AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura +2 more
openaire +2 more sources
Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes
Frontiers in Physiology, 2021 Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes.
Wei Na +24 more
doaj +1 more source
Scientific Reports, 2020 Selecting suitable internal reference genes is an important prerequisite for the application of quantitative real-time PCR (qRT-PCR). However, no systematic studies have been conducted on reference genes in luffa.
mindong chen +7 more
semanticscholar +1 more source