Results 11 to 20 of about 2,306,220 (297)
Identification of Ginger (Zingiber officinale Roscoe) Reference Genes for Gene Expression Analysis [PDF]
Frontiers in Genetics, 2020 Quantitative real-time PCR (qRT-PCR) is widely used in the detection of gene expression level. However, there is no suitable ginger reference gene for qPCR analysis.
Yao Lv +9 more
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Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]
BMC Genomics, 2019 Abstract Background RNA sequencing allows the measuring of gene expression at a resolution unmet by expression arrays or RT-qPCR. It is however necessary to normalize sequencing data by library size, transcript size and composition, among other factors, before comparing expression levels.
Karen Cristine Gonçalves Dos Santos +2 more
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Reference genes in real-time PCR [PDF]
Journal of Applied Genetics, 2013 This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Kozera, Bartłomiej, Rapacz, Marcin
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RefPrimeCouch—a reference gene primer CouchApp [PDF]
Database, 2013 To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive.
Silbermann, Jascha +3 more
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Reference genes for measuring mRNA expression [PDF]
Theory in Biosciences, 2012 The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Dundas, J, Ling, M
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Identification of stable senescence‐associated reference genes [PDF]
Aging Cell, 2019 AbstractCellular senescence is a state of permanent cell cycle arrest activated in response to damaging stimuli. Many hallmarks associated with senescent cells are measured by quantitative real‐time PCR (qPCR). As the selection of stable reference genes for interpretation of qPCR data is often overlooked, we performed a systematic review to understand ...
Alejandra Hernandez‐Segura +2 more
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Reference-based QUantification Of gene Dispensability (QUOD) [PDF]
Plant Methods, 2020 Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina +2 more
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Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
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Reference genes for gene expression studies in the mouse heart [PDF]
Scientific Reports, 2017 AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián +5 more
openaire +6 more sources
Animals, 2021 As the largest chamber of the ruminant stomach, the rumen not only serves as the principal absorptive surface and nutrient transport pathway from the lumen into the animal, but also plays an important short-chain fatty acid (SCFA) metabolic role in ...
Juan Zhao +11 more
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