Results 261 to 270 of about 2,306,220 (297)

Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica

Molecular Biology Reports, 2021
Quantitative gene expression analysis by qPCR requires reference genes for normalization. Lagerstroemia indica (crape myrtle) is a popular ornamental plant in the world, but suitable endogenous reference genes are lacking. To find suitable reference genes, we evaluated the stabilities of nine candidate genes in six experimental data sets: six different
Manli Chen, Qing Wang, Ya Li, Lulu Gao, Fenni Lv, Rutong Yang, Peng Wang   +6 more
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Candidate reference genes for gene expression studies in water lily

Analytical Biochemistry, 2010
The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder.
Huolin, Luo, Sumei, Chen, Hongjian, Wan, Fadi, Chen, Chunsun, Gu, Zhaolei, Liu   +5 more
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Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis

2021
Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference gene is critically important for gene expression analysis due to that wrong reference genes will cause misleading and even wrong conclusion. A good
Xiaoping, Pan, Baohong, Zhang
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Selection of reference genes for gene expression studies in astrocytomas

Analytical Biochemistry, 2011
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene.
Sylwia M, Gresner, Ewa, Golanska, Dominika, Kulczycka-Wojdala, Dariusz J, Jaskolski, Wielislaw, Papierz, Pawel P, Liberski   +5 more
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Rice Reference Genes: redefining reference genes in rice by mining RNA-seq datasets

Plant and Cell Physiology
Abstract Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression.
Xin Liu, Siyuan Tang, Yingbo Gao, Xiaoxiang Zhang, Guichun Dong, Juan Zhou, Yong Zhou, Zefeng Yang, Jianye Huang, Youli Yao   +9 more
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A reference gene set for chemosensory receptor genes of Manduca sexta

Insect Biochemistry and Molecular Biology, 2015
The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context ...
Koenig, C., Hirsh, A., Bucks, S., Klinner, C., Vogel, H., Shukla, A., Mansfield, J., Morton, B., Hansson, B., Grosse-Wilde, E.   +9 more
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Validation of reference genes in the feline endometrium

Reproductive Biology, 2014
The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).
Ewelina, Jursza, Dariusz J, Skarzynski, Marta J, Siemieniuch   +2 more
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Reference standard for gene therapy closer

Molecular Medicine Today, 2000
On both sides of the Atlantic, new initiatives to establish gene therapy benchmarks could stimulate research into rare conditions and allow researchers to compare their results with unprecedented accuracy.Certainly, there is a need to standardize gene therapy vectors.
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Light Intensity-Dependent Dysregulation of Retinal Reference Genes

2019
The degeneration of photoreceptors is a common hallmark of ocular diseases like retinitis pigmentosa (RP) or age-related macular degeneration (AMD). To experimentally induce photoreceptor degeneration, the light damage paradigm is frequently used. In this study we show that the exposure to high amounts of cool white light (10,000 lux, 1 h) resulted in ...
Christina B, Bielmeier, Sabrina I, Schmitt, Barbara M, Braunger   +2 more
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Evaluating reference genes to normalize gene expression in human epileptogenic brain tissues

Biochemical and Biophysical Research Communications, 2010
Several reference genes have been used to quantify gene expression in human epilepsy surgery tissue. However, their reliability has not been validated in detail, although this is crucial in interpreting epilepsy-related changes of gene expression. We evaluated 12 potential reference genes in neocortical tissues resected from patients with temporal lobe
Stephan, Wierschke, Sylvain, Gigout, Peter, Horn, Thomas-Nicolas, Lehmann, Christoph, Dehnicke, Anja U, Bräuer, Rudolf A, Deisz   +6 more
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