Results 21 to 30 of about 13,061,353 (246)
Reference-based QUantification Of gene Dispensability (QUOD) [PDF]
Plant Methods, 2020 Abstract Background Dispensability of genes in a phylogenetic lineage, e.g. a species, genus, or higher-level clade, is gaining relevance as most genome sequencing projects move to a pangenome level.
Frey, Katharina +2 more
openaire +5 more sources
Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]
, 2013 Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen +13 more
core +10 more sources
Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
core +1 more source
Reference genes for gene expression studies in the mouse heart [PDF]
Scientific Reports, 2017 AbstractTo be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field.
Ruiz-Villalba, Adrián +5 more
openaire +6 more sources
Animals, 2021 As the largest chamber of the ruminant stomach, the rumen not only serves as the principal absorptive surface and nutrient transport pathway from the lumen into the animal, but also plays an important short-chain fatty acid (SCFA) metabolic role in ...
Juan Zhao +11 more
doaj +1 more source
Insects, 2021 Bactrocera dorsalis is a major pest that causes serious damage to many fruits. Although phytosanitary treatment methods have been developed for Bactrocera control, there is a lack of information related to the gene expression pattern of B.
Yue Cao +5 more
doaj +1 more source
Reference Gene Screening for Analyzing Gene Expression Across Goat Tissue [PDF]
Asian-Australasian Journal of Animal Sciences, 2013 Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very important when calibrating the results of real-time quantitative PCR.
Yu Zhang +6 more
openaire +3 more sources
Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
Insects, 2022 Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) is a widely used tool for measuring gene expression; however, its accuracy relies on normalizing the data to one or more stable reference genes.
Ying-Na Pan +6 more
doaj +1 more source
Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]
, 2013 Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra +6 more
core +2 more sources
Applied Sciences, 2022 Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression. The key to quantitative accuracy depends on the stability of the reference genes used for data normalization under different ...
Yajing Wu +7 more
doaj +1 more source