Results 41 to 50 of about 13,061,353 (246)

Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in Nicotiana benthamiana upon Single Infections by 11 Positive-Sense Single-Stranded RNA Viruses from Four Genera

Plants, 2023
Quantitative real-time PCR (RT-qPCR) is a widely used method for studying alterations in gene expression upon infections caused by diverse pathogens such as viruses.
Ge Zhang, Zhuo Zhang, Qionglian Wan, Huijie Zhou, Mengting Jiao, Hongying Zheng, Yuwen Lu, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan, Jiejun Peng, Jian Wu   +12 more
doaj   +1 more source

Identification of reliable reference genes for quantitative real‐time PCR analysis of the Rhus chinensis Mill. leaf response to temperature changes

FEBS Open Bio, 2021
Rhus chinensis Mill. (RCM) is the host plant of Galla chinensis, which is valued in traditional medicine. Environmental temperature directly determines the probability of gallnut formation and RCM growth.
Yanchao Chen, Biao Luo, Chuwei Liu, Zhengfeng Zhang, Chi Zhou, Ting Zhou, Guoping Peng, Xujun Wang, Waichin Li, Chuan Wu, Liqun Rao, Qiming Wang   +11 more
doaj   +1 more source

Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant, Monomorium pharaonis (Hymenoptera: Formicidae)

Frontiers in Physiology, 2022
Ants are highly diverse social insects living in colonies consisted of up to millions of individuals with reproductive division of labors. Due to the interests in disclosing the genetic and epigenetic regulation mechanisms underlying the distinct ...
Guo Ding, Guo Ding, Guo Ding, Qionghua Gao, Qionghua Gao, Jun Chen, Jie Zhao, Guojie Zhang, Guojie Zhang, Guojie Zhang, Weiwei Liu   +10 more
doaj   +1 more source

Oculomotor Nucleus [PDF]

, 2008
This report contains a gene expression summary of the oculomotor nucleus, derived from the Allen Brain Atlas (ABA) in situ hybridization mouse data set.
Allen Institute for Brain Science, Angela L. Guillozet-Bongaarts, Josh J. Burnell, Lydia L. Ng   +3 more
core   +2 more sources

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

PLoS ONE, 2018
Quantitative real-time polymerase chain reaction (qRT-PCR) is a prevalent method for gene expression analysis, depending on the stability of the reference genes for data normalization. Lagerstroemia indica and L.
Tangchun Zheng, Zhilin Chen, Yiqian Ju, Han Zhang, Ming Cai, H. Pan, Qixiang Zhang   +6 more
semanticscholar   +1 more source

Gene‐specific dye bias in microarray reference designs [PDF]

FEBS Letters, 2004
The most widely used microarray experiment design includes the use of a reference standard. Comparisons of gene expression between samples are facilitated because each sample is directly measured against the reference standard, using two fluorescent dyes.
Dombkowski, Alan A., Thibodeau, Bryan J., Starcevic, Susan L., Novak, Raymond F.   +3 more
openaire   +2 more sources

Identification, expression analysis, and target prediction of flax genotroph microRNAs under normal and nutrient stress conditions

Frontiers in Plant Science, 2016
Cultivated flax (Linum usitatissimum L.) is an important plant valuable for industry. Some flax lines can undergo heritable phenotypic and genotypic changes (LIS-1 insertion being the most common) in response to nutrient stress and are called plastic ...
Nataliya V. Melnikova, Alexey A. Dmitriev, Maxim S. Belenikin, Maxim S. Belenikin, Nadezhda V. Koroban, Anna S. Speranskaya, Anna S. Speranskaya, Anastasia A. Krinitsina, George S. Krasnov, Valentina A. Lakunina, Anastasiya V. Snezhkina, Asiya F. Sadritdinova, Natalya V. Kishlyan, Tatiana A. Rozhmina, Kseniya M. Klimina, Аlexandra V. Amosova, Alexander V. Zelenin, Olga V. Muravenko, Nadezhda L. Bolsheva, Anna V. Kudryavtseva   +19 more
doaj   +1 more source

Reference Gene Selection for Quantitative Real-Time PCR of Mycelia from Lentinula edodes under High-Temperature Stress

BioMed Research International, 2018
Housekeeping genes are important for measuring the transcription expression of functional genes; 10 traditional reference genes, TUB, TUA, GADPH, EF1, 18S, GTP, ACT, UBI, UBC, and H2A, were tested for their adequacy in Lentinula edodes (L. edodes). Using
Xu Zhao, Huanling Yang, Mingjie Chen, Xiaoxia Song, Changxia Yu, Yan Zhao, Yuejin Wu   +6 more
semanticscholar   +1 more source

With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression Studies

PLOS ONE, 2015
The choice of reference genes that are stably expressed amongst treatment groups is a crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have specified that a minimum of two validated reference genes should be used for normalisation.
Joanne R Chapman, Jonas Waldenström
openaire   +4 more sources

Dentate Gyrus [PDF]

, 2008
This report contains a gene expression summary of the dentate gyrus (DG), derived from the Allen Brain Atlas (ABA) _in situ_ hybridization mouse data set.
Allen Institute for Brain Science, Angela L. Guillozet-Bongaarts, Lydia L. Ng, Rachel A. Dalley   +3 more
core   +2 more sources