Results 51 to 60 of about 2,311,393 (299)
Approach for defining endogenous reference genes in gene expression experiments
Analytical Biochemistry, 2004 The quantification of gene expression by real-time polymerase chain reaction (PCR) has revolutionized the field of gene expression analysis. Due to its sensitivity and flexibility it is becoming the method of choice for many investigators. However, good normalization protocols still have to be implemented to facilitate data exchange and comparison.
Garcia Vallejo, J.J. +6 more
openaire +3 more sources
ERgene: Python library for screening endogenous reference genes [PDF]
Scientific Reports, 2020 AbstractIn gene expression analysis, sample differences and experimental operation differences are common, but sometimes, these differences will cause serious errors to the results or even make the results meaningless. Finding suitable internal reference genes efficiently to eliminate errors is a challenge.
Zeng, Zehua +3 more
openaire +2 more sources
Frontiers in Physiology, 2018 Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Jing Lü +7 more
doaj +1 more source
Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]
Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus +50 more
core +1 more source
, 2008 This report contains a summary of expression patterns for genes that are enriched in the facial motor nucleus (VII) of the medulla. All data is derived from the Allen Brain Atlas (ABA) in situ hybridization mouse project.
Allen Institute for Brain Science +3 more
core +2 more sources
VaccinesBackground/Objectives: The SARS-CoV-2 pandemic challenged patients with inflammatory bowel disease (IBD) under immunosuppressive therapies. We used data from the RisCoin cohort to investigate factors associated with a poor immune response to mRNA ...
Katarina Csollarova +14 more
doaj +1 more source
Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]
Journal of Medical Biochemistry, 2013 Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena +3 more
doaj
Finding regulatory modules through large-scale gene-expression data analysis
, 2004 The use of gene microchips has enabled a rapid accumulation of gene-expression data. One of the major challenges of analyzing this data is the diversity, in both size and signal strength, of the various modules in the gene regulatory networks of ...
Kloster, Morten +2 more
core +1 more source
In-Silico Proportional-Integral Moment Control of Stochastic Gene Expression
, 2020 The problem of controlling the mean and the variance of a species of interest in a simple gene expression is addressed. It is shown that the protein mean level can be globally and robustly tracked to any desired value using a simple PI controller that ...
Briat, Corentin, Khammash, Mustafa
core +1 more source
, 2008 This report contains a gene expression summary of the area postrema (AP), derived from the "Allen Brain Atlas":http://www.brain-map.org/welcome.do;jsessionid=EDE40ADC940845D169DE378ADC9B71BD (ABA) in-situ hybridization (ISH) mouse data set ...
Allen Institute for Brain Science +3 more
core +2 more sources