Results 71 to 80 of about 13,061,353 (246)

Selection and Validation of Reference Genes for RT-qPCR Analysis of the Ladybird Beetle Henosepilachna vigintioctomaculata

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a momentous technique for quantifying expression levels of the targeted genes across various biological processes.
Jing Lü, Shimin Chen, Mujuan Guo, Cuiyi Ye, Baoli Qiu, Jianhui Wu, Chunxiao Yang, Huipeng Pan   +7 more
doaj   +1 more source

The Sorghum bicolor reference genome: improved assembly, gene annotations, a transcriptome atlas, and signatures of genome organization. [PDF]

, 2017
Sorghum bicolor is a drought tolerant C4 grass used for the production of grain, forage, sugar, and lignocellulosic biomass and a genetic model for C4 grasses due to its relatively small genome (approximately 800 Mbp), diploid genetics, diverse ...
Amirebrahimi, Mojgan, Grimwood, Jane, Jenkins, Jerry, Kennedy, Megan, Mattison, Ashley, McCormick, Ryan F, McKinley, Brian, Morishige, Daryl T, Mullet, John E, Schmutz, Jeremy, Shu, Shengqiang, Sims, David, Sreedasyam, Avinash, Truong, Sandra K, Weers, Brock D   +14 more
core   +2 more sources

Factors Associated with Impaired Humoral Immune Response to mRNA Vaccines in Patients with Inflammatory Bowel Disease: A Matched-Cohort Analysis from the RisCoin Study

Vaccines
Background/Objectives: The SARS-CoV-2 pandemic challenged patients with inflammatory bowel disease (IBD) under immunosuppressive therapies. We used data from the RisCoin cohort to investigate factors associated with a poor immune response to mRNA ...
Katarina Csollarova, Leandra Koletzko, Thu Giang Le Thi, Paul R. Wratil, Ana Zhelyazkova, Simone Breiteneicher, Marcel Stern, Gaia Lupoli, Tobias Schwerd, Alexander Choukér, Veit Hornung, Oliver T. Keppler, Kristina Adorjan, Helga Paula Török, Sibylle Koletzko   +14 more
doaj   +1 more source

Gene expression studies: How to obtain accurate and reliable data by quantitative real-time RT PCR [PDF]

Journal of Medical Biochemistry, 2013
Real-time RT PCR has been recognized as an accurate, reliable and sensitive method for quantifying gene transcription. However, several steps preceding PCR represent critical points and source of inaccuracies.
Nestorov Jelena, Matić Gordana, Elaković Ivana, Tanić Nikola   +3 more
doaj  

Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation [PDF]

, 2005
The Scientific Committee of Molecular Biology Techniques (C-MbT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission, Joint Research Centre, Institute of Reference Materials and Measurements to ...
Cooper, S., Corbisier, Philippe, Gancberg, David, Gemmati, Donato, Halbmayer, Walter-Michael, Kitchen, Steve, Klein, Christoph L., Mannhalter, Christine, Marki-Zay, János, Melegh, Béla, Neumaier, Michael, Oldenburg, Johannes, Opplinger Leibundgut, Elisabeth, Reitsma, Pieter H., Rieger, Sandra, Schimmel, Heinz G., Spannagl, Michael, Tordai, Attilia, Tosetto, Alberto, Visvikis, Sophie, Zadro, Renata   +20 more
core   +4 more sources

Reference Gene Selection for RT-qPCR Analysis of Flower Development in Chrysanthemum morifolium and Chrysanthemum lavandulifolium

Frontiers in Plant Science, 2016
Quantitative real-time PCR (qPCR) is a popular and powerful tool used to understand the molecular mechanisms of flower development. However, the accuracy of this approach depends on the stability of reference genes.
Shuai Qi, Liwen Yang, Xiaohui Wen, Yan Hong, Xuebin Song, Mengmeng Zhang, S. Dai   +6 more
semanticscholar   +1 more source

Validation of reference genes in human chordoma

Surgical Neurology International, 2017
Chordoma are rare slow-growing tumors of the axial skeleton, which are thought to arise from remnants of the notochord. Little is known about the underlying mechanisms that drive this tumor. However, the assessment of gene expression levels by quantitative real-time polymerase chain reaction (qRT-PCR) is hampered due to a lack of validated reference ...
Santegoeds, R G C, Yakkioui, Y, Jahanshahi, A, Hoogland, G, Temel, Y, van Overbeeke, J J   +5 more
openaire   +3 more sources

An alternative to Kitcher's theory of conceptual progress and his account of the change of the gene concept [PDF]

, 2004
The present paper discusses Kitcher’s framework for studying conceptual change and progress. Kitcher’s core notion of reference potential is hard to apply to concrete cases.
Brigandt, Ingo
core   +1 more source

Genome-Wide Identification, Functional Analysis and Expression Profiling of the Aux/IAA Gene Family in Tomato [PDF]

, 2012
Auxin is a central hormone that exerts pleiotropic effects on plant growth including the development of roots, shoots, flowers and fruit. The perception and signaling of the plant hormone auxin rely on the cooperative action of several components,among
Abel, Abel, Abel, Bargmann, Carole Bassa, Chaabouni, Chaabouni, Corinne Audran-Delalande, Deng, Dharmasiri, Dharmasiri, Dreher, Eshed, Farid Regad, Finn, Hagen, Hamilton, Isabelle Mila, Jain, Jones, Kagale, Kalluri, Kepinski, Kloosterman, Leclercq, Li, Liscum, Mohamed Zouine, Mondher Bouzayen, Montgomery, Nebenführ, Oeller, Ohme-Takagi, Ottenschläger, Overvoorde, Paponov, Pirrello, Reed, Remington, Saitou, Sato, Szemenyei, Tamura, Tan, Theologis, Tiwari, Tiwari, Uehara, Ulmasov, Ulmasov, Wang, Wang, Wang, Wang, Yamamoto, Zuckerkandl   +55 more
core   +4 more sources

Reference Genes for Quantitative Gene Expression Studies in Multiple Avian Species

PLoS ONE, 2014
Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data.
Olias, Philipp, Adam, Iris, Meyer, Anne, Scharff, Constance, Gruber, Achim Dieter   +4 more
openaire   +6 more sources