Results 81 to 90 of about 13,061,353 (246)

Evaluation of reference genes for insect olfaction studies [PDF]

Parasites & Vectors, 2015
La PCR cuantitativa de transcripción inversa (qRT-PCR) es un método robusto y accesible para analizar la expresión génica e inferir la regulación génica. Al ser una cadena de procedimientos, esta técnica está sujeta a errores sistemáticos debido a limitaciones biológicas y técnicas establecidas principalmente por el material de partida y los ...
Bonaventure Aman Omondi, José Manuel Latorre-Estivalis, Ivana Helena Rocha Oliveira, Rickard Ignell, Marcelo Gustavo Lorenzo   +4 more
openaire   +4 more sources

Identification of the Valid Reference Genes for Quantitative RT-PCR in Annual Ryegrass (Lolium multiflorum) under Salt Stress

Molecules, 2015
Annual ryegrass (Lolium multiflorum) is a cool-season annual grass cultivated worldwide for its high yield and quality. With the areas of saline soil increasing, investigation of the molecular mechanisms of annual ryegrass tolerance under salt stress has
Xia Wang, Xiao Ma, Linkai Huang, Xinquan Zhang   +3 more
doaj   +1 more source

Validation of suitable internal control genes for expression studies in aging. [PDF]

, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA, Burkle, A, Caiafa, Paola, Calabrese, Roberta, Chevanne, M, Ciccarone, Fabio, Guastafierro, Tiziana, MORENO VILLANUEVA, M, Reale, Anna, Zampieri, Michele   +9 more
core   +1 more source

Reference genes for livestock gene expression profiling – Literature review

Acta Agraria Debreceniensis, 2017
Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the ...
Ádám Simon, Zsuzsa Zakarné Aszalós, András Jávor, Levente Czeglédi   +3 more
openaire   +2 more sources

Selection of reference genes for RT-qPCR analysis in Coccinella septempunctata to assess un-intended effects of RNAi transgenic plants

Frontiers in Plant Science, 2016
The development of genetically-engineered plants that employ RNA interference (RNAi) to suppress invertebrate pests opens up new avenues for insect control.
Chunxiao Yang, Chunxiao Yang, Chunxiao Yang, Evan L. Preisser, Hongjun Zhang, Hongjun Zhang, Yong Liu, Liangying Dai, Huipeng Pan, Huipeng Pan, Xuguo Zhou   +10 more
doaj   +1 more source

Validating reference genes using minimally transformed qpcr data: findings in human cortex and outcomes in schizophrenia [PDF]

, 2016
BACKGROUND: It is common practice, when using quantitative real time polymerase chain reaction (qPCR), to normalise levels of mRNA to reference gene mRNA which, by definition, should not vary between tissue, with any disease aetiology or after drug ...
Brian Dean, Elizabeth Scarr, Madhara Udawela   +2 more
core   +4 more sources

Reference gene selection for cross-species and cross-ploidy level comparisons in Chrysanthemum spp.

Scientific Reports, 2015
The establishment of a (set of) stably expressed reference gene(s) is required to normalize transcription data. Polyploidy is very common in the plant kingdom, but it is not necessarily the case that a reference gene which works well at the diploid level
Haibin Wang, Sumei Chen, Jiafu Jiang, Fei Zhang, Fadi Chen   +4 more
semanticscholar   +1 more source

AutDB: a gene reference resource for autism research [PDF]

Nucleic Acids Research, 2008
Recent advances in studies of Autism Spectrum Disorders (ASD) has uncovered many new candidate genes and continues to do so at an accelerated pace. To address the genetic complexity of ASD, we have developed AutDB (http://www.mindspec.org/autdb.html), a publicly available web-portal for on-going collection, manual annotation and visualization of genes ...
Basu, Saumyendra N., Kollu, Ravi, Banerjee-Basu, Sharmila   +2 more
openaire   +2 more sources

Selection and validation of reference genes for RT-qPCR-based analyses of Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae)

Frontiers in Physiology, 2022
RT-qPCR remains a vital approach for molecular biology studies aimed at quantifying gene expression in a range of physiological or pathological settings.
Zixin Liu, Zixin Liu, Junjiang Xiao, Junjiang Xiao, Yue Xia, Qifeng Wu, Qifeng Wu, Can Zhao, Dunsong Li   +8 more
doaj   +1 more source

1000 Norms Project: Protocol of a cross-sectional study cataloging human variation [PDF]

, 2015
Background Clinical decision-making regarding diagnosis and management largely depends on comparison with healthy or ‘normal’ values. Physiotherapists and researchers therefore need access to robust patient-centred outcome measures and appropriate ...
Baldwin, Jennifer N., Burns, Joshua, Chard, Angus, Ferreira, Paulo, Fong Yan, Alycia, Hiller, Claire E., Hübscher, Markus, Mackey, Martin G., McKay, Marnee J., Moloney, Niamh A., Mousavi, Seyed J., Nicholson, Leslie L., Nightingale, Elizabeth J., on behalf of the 1000 Norms Project Consortium, Pourkazemi, Fereshteh, Quinlan, Kate G., Raymond, Jacqueline, Refshauge, Kathryn M., Rose, Kristy, Simic, Milena, Sman, Amy D., Vanicek, Natalie, Wegener, Caleb   +22 more
core   +2 more sources