Results 11 to 20 of about 34,862 (253)
eLife, 2023 Antiviral defense in ecdysozoan invertebrates requires Dicer with a helicase domain capable of ATP hydrolysis. But despite well-conserved ATPase motifs, human Dicer is incapable of ATP hydrolysis, consistent with a muted role in antiviral defense.
Adedeji M Aderounmu +3 more
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Characterization of the biochemical properties of recombinant ribonuclease III [PDF]
Nucleic Acids Research, 1990 An Escherichia coli double strand specific endoribonuclease, RNase III, was cloned, expressed in large amounts, and purified to homogeneity. Enzyme activity was monitored by assaying fractions for the ability to correctly process exogenous RNA containing specific RNase III cleavage sites.
P E, March, M A, Gonzalez
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Preliminary studies on ribonucleases from Poa pratensis seeds
Acta Societatis Botanicorum Poloniae, 2015 Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1.
Irena Lorenc-Kubis +1 more
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Nature Communications, 2021 Type III CRISPR-Cas systems recognize and cleave target RNAs and produce signalling molecules. Here the authors discover that both processes are governed by a flexible seed region, ultimately resulting in SCOPE, a SARSCoV-2 diagnostic assay with atto ...
Jurre A. Steens +12 more
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Cells, 2019 Idiopathic pulmonary fibrosis (IPF) has been linked to chronic lung inflammation. Drosha ribonuclease III (DROSHA), a class 2 ribonuclease III enzyme, plays a key role in microRNA (miRNA) biogenesis.
Soo Jung Cho +6 more
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International Journal of Endocrinology, 2020 As a type III ribonuclease (RNase III) specifically cleaving double-stranded RNA substrates into short fragments, Dicer is indispensable in a range of physi/pathologic processes, e.g., nutrient deprivation, hypoxia, or DNA damage.
Xiaohui Tong +3 more
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Mouse ribonuclease III. cDNA structure, expression analysis, and chromosomal location
BMC Genomics, 2002 Background Members of the ribonuclease III superfamily of double-stranded(ds)-RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells. A human RNase III orthologue has been implicated in
Nicholson Rhonda H +2 more
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Ribonuclease III Processing of Coaxially Stacked RNA Helices [PDF]
Journal of Biological Chemistry, 1999 The RNase III family of endoribonucleases participates in maturation and decay of cellular and viral transcripts by processing of double-stranded RNA. RNase III degradation is inherent to most antisense RNA-regulated gene systems in Escherichia coli. In the hok/sok system from plasmid R1, Sok antisense RNA targets the hok mRNA for RNase III-mediated ...
Franch, T., Thisted, T., Gerdes, K.
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Dicer Is Required for Normal Cerebellar Development and to Restrain Medulloblastoma Formation. [PDF]
PLoS ONE, 2015 Dicer, a ribonuclease III enzyme, is required for the maturation of microRNAs. To assess its role in cerebellar and medulloblastoma development, we genetically deleted Dicer in Nestin-positive neural progenitors and in mice lacking one copy for the Sonic
Frederique Zindy +7 more
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Sinorhizobium meliloti RNase III: Catalytic Features and Impact on Symbiosis
Frontiers in Genetics, 2018 Members of the ribonuclease (RNase) III family of enzymes are metal-dependent double-strand specific endoribonucleases. They are ubiquitously found and eukaryotic RNase III-like enzymes include Dicer and Drosha, involved in RNA processing and RNA ...
Margarida Saramago +4 more
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