Results 21 to 30 of about 5,666,730 (325)
Accurate profiling of single-cell alternative transcript start sites by correcting RNA degradation [PDF]
Nature CommunicationsThe generation of transcript variants via alternative utilisation of transcription start sites (TSSs) is a pivotal regulatory mechanism in physiological and pathological states.
Zijie Xu +15 more
doaj +2 more sources
Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing [PDF]
Nature Communications, 2023 Observing the natural process of RNA degradation in real-time is a significant challenge. Here, the authors develop and use single-exonuclease nanocircuits to reveal the single-base degradation behaviour of PNPase, and demonstrate proof-of-principle RNA ...
Zhiheng Yang +6 more
doaj +2 more sources
A dedicated diribonuclease resolves a key bottleneck for the terminal step of RNA degradation
eLife, 2019 Degradation of RNA polymers, an ubiquitous process in all cells, is catalyzed by specific subsets of endo- and exoribonucleases that together recycle RNA fragments into nucleotide monophosphate.
Soo-Kyoung Kim +8 more
doaj +2 more sources
Impact of RNA degradation on fusion detection by RNA-seq
BMC Genomics, 2016 RNA-seq is a well-established method for studying the transcriptome. Popular methods for library preparation in RNA-seq such as Illumina TruSeq® RNA v2 kit use a poly-A pulldown strategy.
Jaime I. Dávila +14 more
semanticscholar +3 more sources
RNA-seq: impact of RNA degradation on transcript quantification [PDF]
BMC Biology, 2014 The use of low quality RNA samples in whole-genome gene expression profiling remains controversial. It is unclear if transcript degradation in low quality RNA samples occurs uniformly, in which case the effects of degradation can be corrected via data ...
Irene Gallego Romero +3 more
semanticscholar +5 more sources
RNA degradation in antiviral immunity and autoimmunity
Trends in Immunology, 2015 Highlights • The nonsense-mediated decay (NMD) pathway defends cells against RNA virus invasion.• NMD targets viral RNAs for degradation, including by the RNA exosome.• Genetic deficiencies in NMD and RNA exosome components cause autoimmunity.• NMD and ...
R. Rigby, J. Rehwinkel
semanticscholar +3 more sources
RNA‐PROTACs: Degraders of RNA‐Binding Proteins [PDF]
Angewandte Chemie International Edition, 2020 AbstractDefects in the functions of RNA binding proteins (RBPs) are at the origin of many diseases; however, targeting RBPs with conventional drugs has proven difficult. PROTACs are a new class of drugs that mediate selective degradation of a target protein through a cell's ubiquitination machinery.
Alice Ghidini +4 more
openaire +6 more sources
RNA Biology, 2023 Histone mRNA degradation is controlled by the unique 3’ stem-loop of histone mRNA and the stem-loop binding protein (SLBP). As part of this process, the 3’ stem-loop is trimmed by the histone-specific 3’ exonuclease (3’hExo) and uridylated by the ...
Morgan Shine +6 more
doaj +1 more source
Plants, 2020 RNA quality control is an indispensable but poorly understood process that enables organisms to distinguish functional RNAs from nonfunctional or inhibitory ones.
Amber M. Hotto +2 more
doaj +1 more source