Results 91 to 100 of about 3,502 (177)

The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome [PDF]

RNA, 2010
The Escherichia coli endoribonuclease RNase E is an essential enzyme having key roles in mRNA turnover and the processing of several structured RNA precursors, and it provides the scaffold to assemble the multienzyme RNA degradosome. The activity of RNase E is inhibited by the protein RraA, which can interact with the ribonuclease's degradosome ...
Gorna, M., Pietras, Z., Tsai, Y., Callaghan, Anastasia, Hernandez, H., Robinson, Carol V., Luisi, B.   +6 more
openaire   +3 more sources

Phase Separation Regulates Metabolism, Mitochondria, and Diseases

MedComm, Volume 6, Issue 7, July 2025.
Mitochondrion‐related liquid–liquid phase separation. (1) Phase separation mediates the self‐assembly of mitochondrial nucleoids (mt‐nucleoids). (2) Phase segregation mediates the formation of mitochondrial RNA granules (MRGs). (3) Phase separation mediates the formation of mitochondrial degradosomes.
Chuan Gao, Peng Ding, Changqing Zhang, Junjie Gao   +3 more
wiley   +1 more source

The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 [PDF]

, 2014
BACKGROUND: RNA metabolism, including RNA synthesis and RNA degradation, is one of the most conserved biological systems and has been intensively studied; however, the degradation network of ribonucleases (RNases) and RNA substrates is not fully ...
Akeo Shinkai, Hiromasa Ohyama, Kenji Fukui, Noriko Nakagawa, Ryoji Masui, Seiki Kuramitsu, Tomofumi Sakai, Yoshihiro Agari   +7 more
core   +1 more source

A mutation in polynucleotide phosphorylase from Escherichia coli impairing RNA binding and degradosome stability [PDF]

Nucleic Acids Research, 2004
Polynucleotide phosphorylase (PNPase), a 3' to 5' exonuclease encoded by pnp, plays a key role in Escherichia coli RNA decay. The enzyme, made of three identical 711 amino acid subunits, may also be assembled in the RNA degradosome, a heteromultimeric complex involved in RNA degradation.
M.E. Regonesi, F. Briani, A. Ghetta, S. Zangrossi, D. Ghisotti, P. Tortora, G. Dehò   +6 more
openaire   +5 more sources

The Power Duo: How the Interplay Between Nucleoid‐Associated Proteins and Small Noncoding RNAs Orchestrates the Cellular Regulatory Symphony

Molecular Microbiology, Volume 123, Issue 6, Page 531-546, June 2025.
Nucleoid‐associated proteins play a crucial role in the compaction of bacterial DNA and the regulation of gene expression. The emergence of small noncoding RNAs as regulatory elements in these processes represents a major development in our understanding of bacterial biology.
Sara Moutacharrif, Feth El Zahar Haichar, Sam Meyer, Cecile Ribot, Sylvie Reverchon, William Nasser, Florence Hommais   +6 more
wiley   +1 more source

Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa [PDF]

, 2017
Polyphosphate (polyP) granule biogenesis is an ancient and ubiquitous starvation response in bacteria. Although the ability to make polyP is important for survival during quiescence and resistance to diverse environmental stresses, granule genesis is ...
Dieterle, Michael G., Jensen, Grant J., Newman, Dianne K., Racki, Lisa R., Sullivan, Meaghan C., Tocheva, Elitza I.   +5 more
core   +1 more source

Polyphosphate: The “Dark Matter” of Bacterial Chromatin Structure

Molecular Microbiology, Volume 123, Issue 3, Page 279-293, March 2025.
Polyphosphate condensates and their contributions to nucleoid structure. Polyphosphate condensates form in the ribosome‐depleted nucleoid region of bacterial cells, particularly under stress (in this case Pseudomonas aeruginosa undergoing nitrogen starvation; adapted with permission from (Racki et al. 2017)). This Perspective explores the emerging view
Lisa R. Racki, Lydia Freddolino
wiley   +1 more source

Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome

, 2020
The method of co-immunoprecipitation (co-IP or pulldown) enables the identification of proteins interacting in macromolecular assemblies, through the purification of a key protein by affinity chromatography using specific antibodies immobilized on a matrix.
Steven W, Hardwick, Ben F, Luisi, Marilis V, Marques   +2 more
openaire   +3 more sources

Effects of lipid membranes on RNA catalytic activity and stability

Biology of the Cell, Volume 117, Issue 2, February 2025.
This study explores how lipid membranes influence the activity and stability of natural ribozymes. We found that lipid membranes can both decrease and enhance ribozyme activity. We also observed that lipid membranes promote RNA degradation, depending on lipid composition and membrane fluidity.
Tomasz Czerniak, James P. Saenz
wiley   +1 more source

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

eLife, 2019
CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj   +1 more source