Detection of RNA modifications [PDF]
RNA Biology, 2010 RNA nucleotide modifications are typically of low abundance and frequently go unnoticed by standard detection methods of molecular biology and cell biology. With a burst of knowledge intruding from such diverse areas as genomics, structural biology, regulation of gene expression and immunology, it becomes increasingly clear that many exciting functions
Stefanie, Kellner +2 more
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RNA modifications detection by comparative Nanopore direct RNA sequencing
Nature Communications, 2021 Nanopore direct RNA Sequencing data contain information about the presence of RNA modifications, but their detection poses substantial challenges. Here the authors introduce Nanocompore, a new methodology for modification detection from Nanopore data.
Adrien Leger +18 more
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Direct detection of RNA modifications and structure using single-molecule nanopore sequencing
Cell Genomics, 2022 Summary: Modifications are present on many classes of RNA, including tRNA, rRNA, and mRNA. These modifications modulate diverse biological processes such as genetic recoding and mRNA export and folding. In addition, modifications can be introduced to RNA
William Stephenson +5 more
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Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in "Saccharomyces cerevisiae" [PDF]
, 2013 The 25S rRNA of yeast contains several base modifications in the functionally important regions. The enzymes responsible for most of these base modifications remained unknown.
Entian, Karl-Dieter +3 more
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Post-Transcriptional Regulation of Viral RNA through Epitranscriptional Modification
Cells, 2021 The field of mRNA modifications has been steadily growing in recent years as technologies have improved and the importance of these residues became clear.
David G. Courtney
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RNA modifications can affect RNase H1-mediated PS-ASO activity
Molecular Therapy: Nucleic Acids, 2022 Phosphorothioate modified antisense oligonucleotides (PS-ASOs) can reduce gene expression through hybridization to target RNAs and subsequent cleavage by RNase H1.
Katelyn A. Doxtader Lacy +3 more
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Deciphering the epitranscriptome: a green perspective [PDF]
, 2016 The advent of high-throughput sequencing technologies coupled with new detection methods of RNA modifications has enabled investigation of a new layer of gene regulation - the epitranscriptome.
Burgess, A., David, R., Searle, I.
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RNA modification mapping with JACUSA2
Genome Biology, 2022 Abstract Several high-throughput antibody-free methods for RNA modification detection from sequencing data have been developed. We present JACUSA2 as a versatile software solution and comprehensive analysis framework for RNA modification detection assays that are based on either the Illumina or Nanopore platform ...
Michael Piechotta +4 more
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New insights into the biological role of mammalian ADARs; the RNA editing proteins [PDF]
, 2015 The ADAR proteins deaminate adenosine to inosine in double-stranded RNA which is one of the most abundant modifications present in mammalian RNA.
Arieti, Fabiana +4 more
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A New Epigenetic Crosstalk: Chemical Modification Information Flow
Advanced Genetics, 2023 Central dogma is the most fundamental hypothesis in the field of molecular biology and explains the genetic information flow from DNA to protein. Beyond residue‐by‐residue transmission of sequential information, chemical modifications of DNA, RNA, and ...
Hongwoo Lee +2 more
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