Results 1 to 10 of about 490,934 (250)

Pericentrin-mediated SAS-6 recruitment promotes centriole assembly [PDF]

eLife, 2019
The centrosome is composed of two centrioles surrounded by a microtubule-nucleating pericentriolar material (PCM). Although centrioles are known to regulate PCM assembly, it is less known whether and how the PCM contributes to centriole assembly. Here we
Daisuke Ito, Sihem Zitouni, Swadhin Chandra Jana, Paulo Duarte, Jaroslaw Surkont, Zita Carvalho-Santos, José B Pereira-Leal, Miguel Godinho Ferreira, Mónica Bettencourt-Dias   +8 more
doaj   +6 more sources

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly [PDF]

Nature Communications, 2021
Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that scaffolds organelle formation. Here, the authors develop monobodies against Chlamydomonas reinhardtii SAS-6 and
Georgios N. Hatzopoulos, Tim Kükenshöner, Niccolò Banterle, Tatiana Favez, Isabelle Flückiger, Virginie Hamel, Santiago Andany, Georg E. Fantner, Oliver Hantschel, Pierre Gönczy   +9 more
doaj   +7 more sources

Structure of the SAS-6 cartwheel hub from Leishmania major [PDF]

eLife, 2014
Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the ...
Mark van Breugel, Rainer Wilcken, Stephen H McLaughlin, Trevor J Rutherford, Christopher M Johnson   +4 more
doaj   +6 more sources

Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis [PDF]

EMBO Reports
In most metazoans, centrosome elimination during oogenesis ensures accurate centriole inheritance in the zygote, yet the molecular mechanisms remain poorly understood.
Feifei Qi, Shanshan Yin, Xiangrui Yang, Ning Ju, Bohan Liu, Xing Zhang, Zixuan Zhu, Li Ji, Fuxin Zhang, Li Zhao, Ruoxi Wang, Min Liu, Liangran Zhang, Huijie Zhao, Jun Zhou, Jinmin Gao   +15 more
doaj   +4 more sources

Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells [PDF]

eLife, 2022
SAS-6 (SASS6) is essential for centriole formation in human cells and other organisms but its functions in the mouse are unclear. Here, we report that Sass6-mutant mouse embryos lack centrioles, activate the mitotic surveillance cell death pathway, and ...
Marta Grzonka, Hisham Bazzi
doaj   +4 more sources

Poc1B and Sas-6 Function Together during the Atypical Centriole Formation in Drosophila melanogaster [PDF]

Cells, 2019
Insects and mammals have atypical centrioles in their sperm. However, it is unclear how these atypical centrioles form. Drosophila melanogaster sperm has one typical centriole called the giant centriole (GC) and one atypical centriole called the proximal
Kyoung H. Jo, Ankit Jaiswal, Sushil Khanal, Emily L. Fishman, Alaina N. Curry, Tomer Avidor-Reiss   +5 more
doaj   +5 more sources

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6 [PDF]

Nature Communications, 2017
Centrosome duplication is synchronized with cell cycle events but how this is regulated at a molecular level is unclear. Here, the authors show that the DNA replication licensing factor Cdc6 restrains centrosome duplication via binding and inhibiting Sas-
Xiaowei Xu, Shijiao Huang, Boyan Zhang, Fan Huang, Wangfei Chi, Jingyan Fu, Gang Wang, Si Li, Qing Jiang, Chuanmao Zhang   +9 more
doaj   +5 more sources

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture [PDF]

Nature Communications, 2021
The centriole exhibits an evolutionarily conserved 9-fold radial symmetry that stems from a cartwheel containing vertically stacked ring polymers that harbor 9 homodimers of the protein SAS-6. Here the authors show how dual properties inherent to surface-
Niccolò Banterle, Adrian P. Nievergelt, Svenja de Buhr, Georgios N. Hatzopoulos, Charlène Brillard, Santiago Andany, Tania Hübscher, Frieda A. Sorgenfrei, Ulrich S. Schwarz, Frauke Gräter, Georg E. Fantner, Pierre Gönczy   +11 more
doaj   +2 more sources

Generation of sas-6::ha by CRISPR/Cas9 editing. [PDF]

MicroPubl Biol, 2019
Centrosomes are comprised of a pair of barrel-shaped centrioles that are oriented at right angles to each other, and embedded in electron dense pericentriolar material. The centrosomes mediate spindle assembly and function as basal bodies to promote cilia and flagella formation (reviewed in Pintard and Bowerman, 2019 and Fırat-Karalar and Stearns, 2014)
Bergwell M, Smith A, Lakin H, Slay R, Iyer J.   +4 more
europepmc   +3 more sources

Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly. [PDF]

Dev Cell, 2013
Assembly of SAS-6 dimers to form the centriolar cartwheel requires the ZYG-1/Plk4 kinase. Here, we show that ZYG-1 recruits SAS-6 to the mother centriole independently of its kinase activity; kinase activity is subsequently required for cartwheel assembly. We identify a direct interaction between ZYG-1 and the SAS-6 coiled coil that explains its kinase
Lettman MM, Wong YL, Viscardi V, Niessen S, Chen SH, Shiau AK, Zhou H, Desai A, Oegema K.   +8 more
europepmc   +4 more sources