Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization. [PDF]
J Biol Chem, 2020 Centrioles are key eukaryotic organelles that are responsible for the formation of cilia and flagella, and for organizing the microtubule network and the mitotic spindle in animals. Centriole assembly requires oligomerization of the essential protein spindle assembly abnormal 6 (SAS-6), which forms a structural scaffold templating the organization of ...
Busch JMC +5 more
europepmc +5 more sources
The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies [PDF]
eLife, 2015 Sas-6 and Ana2/STIL proteins are required for centriole duplication and the homo-oligomerisation properties of Sas-6 help establish the ninefold symmetry of the central cartwheel that initiates centriole assembly. Ana2/STIL proteins are poorly conserved,
Matthew A Cottee +5 more
doaj +2 more sources
Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel. [PDF]
Structure, 2022 Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9-fold radial symmetry imparted by a likewise symmetrical central scaffold, the cartwheel.
Kantsadi AL +3 more
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De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly [PDF]
eLife, 2015 Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur. Consistently, centriole formation is thought to strictly rely on self-assembly, involving self-oligomerization of the
Won-Jing Wang +5 more
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The PLK4-STIL-SAS-6 module at the core of centriole duplication. [PDF]
Biochem Soc Trans, 2016 Centrioles are microtubule-based core components of centrosomes and cilia. They are duplicated exactly once during S-phase progression. Central to formation of each new (daughter) centriole is the formation of a nine-fold symmetrical cartwheel structure onto which microtubule triplets are deposited.
Arquint C, Nigg EA.
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Self-assembling SAS-6 multimer is a core centriole building block. [PDF]
J Biol Chem, 2010 Centrioles are conserved microtubule-based organelles with 9-fold symmetry that are essential for cilia and mitotic spindle formation. A conserved structure at the onset of centriole assembly is a "cartwheel" with 9-fold radial symmetry and a central tubule in its core. It remains unclear how the cartwheel is formed.
Gopalakrishnan J +6 more
europepmc +5 more sources
The chromatin remodeling protein CHD-1 and the EFL-1/DPL-1 transcription factor cooperatively down regulate CDK-2 to control SAS-6 levels and centriole number. [PDF]
PLoS Genetics, 2022 Centrioles are submicron-scale, barrel-shaped organelles typically found in pairs, and play important roles in ciliogenesis and bipolar spindle assembly.
Jyoti Iyer +10 more
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A dynamically interacting flexible loop assists oligomerisation of the Caenorhabditis elegans centriolar protein SAS-6. [PDF]
Sci Rep, 2019 AbstractCentrioles are conserved organelles fundamental for the organisation of microtubules in animal cells. Oligomerisation of the spindle assembly abnormal protein 6 (SAS-6) is an essential step in the centriole assembly process and may act as trigger for the formation of these organelles.
Busch JMC +5 more
europepmc +5 more sources
SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication. [PDF]
Dev Cell, 2014 Centrioles are 9-fold symmetric structures duplicating once per cell cycle. Duplication involves self-oligomerization of the centriolar protein SAS-6, but how the 9-fold symmetry is invariantly established remains unclear. Here, we found that SAS-6 assembly can be shaped by preexisting (or mother) centrioles.
Fong CS +4 more
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A SAS-6-like protein suggests that the Toxoplasma conoid complex evolved from flagellar components. [PDF]
Eukaryot Cell, 2013 ABSTRACTSAS-6 is required for centriole biogenesis in diverse eukaryotes. Here, we describe a novel family of SAS-6-like (SAS6L) proteins that share an N-terminal domain with SAS-6 but lack coiled-coil tails. SAS6L proteins are found in a subset of eukaryotes that contain SAS-6, including diverse protozoa and green algae.
de Leon JC +9 more
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