Results 11 to 20 of about 52,178 (281)

“Stapling” scFv for multispecific biotherapeutics of superior properties

mAbs, 2023
Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells.
Lauren E. Boucher, Elisabeth Geyer Prinslow, Michael Feldkamp, Fang Yi, Rupesh Nanjunda, Sheng-Jiun Wu, Tun Liu, Eilyn R. Lacy, Steven Jacobs, Natalia Kozlyuk, Brian Del Rosario, Bingyuan Wu, Patricia Aquino, Robert C. Davidson, Samantha Heyne, Nicholas Mazzanti, James Testa, Michael D. Diem, Elsa Gorre, Andrew Mahan, Hirsh Nanda, Harsha P. Gunawardena, Alexis Gervais, Anthony A. Armstrong, Alexey Teplyakov, Chichi Huang, Adam Zwolak, Partha Chowdhury, Wan Cheung Cheung, Jinquan Luo   +29 more
doaj   +2 more sources

Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv. [PDF]

PLoS ONE, 2017
CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E.
Anna Gąciarz, Lloyd W Ruddock
doaj   +2 more sources

A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system

BMC Biotechnology, 2018
Background Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties.
Md. Kausar Alam, Michelle Brabant, Raja Solomon Viswas, Kris Barreto, Humphrey Fonge, C. Ronald Geyer   +5 more
doaj   +2 more sources

Double attack strategy for leukemia using a pre-targeting bispecific antibody (CD20 Ab-mPEG scFv) and actively attracting PEGylated liposomal doxorubicin to enhance anti-tumor activity

Journal of Nanobiotechnology, 2021
Background Tumor-targeted nanoparticles hold great promise as new tools for therapy of liquid cancers. Furthermore, the therapeutic efficacy of nanoparticles can be improved by enhancing the cancer cellular internalization.
Kai-Wen Ho, I.-J.u Chen, Yi-An Cheng, Tzu-Yi Liao, En-Shuo Liu, Huei-Jen Chen, Yun-Chi Lu, Yu-Cheng Su, Steve R. Roffler, Bo-Cheng Huang, Hui-Ju Liu, Ming-Yii Huang, Chiao-Yun Chen, Tian-Lu Cheng   +13 more
doaj   +2 more sources

Target delivery of a PD-1-TREM2 scFv by CAR-T cells enhances anti-tumor efficacy in colorectal cancer

Molecular Cancer, 2023
Background Chimeric antigen receptor (CAR) -T cell therapy is an efficient therapeutic strategy for specific hematologic malignancies. However, positive outcomes of this novel therapy in treating solid tumors are curtailed by the immunosuppressive tumor ...
Jing Chen, Tianchuan Zhu, Guanmin Jiang, Qi Zeng, Zhijian Li, Xi Huang   +5 more
semanticscholar   +1 more source

Mechanisms of Action and Limitations of Monoclonal Antibodies and Single Chain Fragment Variable (scFv) in the Treatment of Cancer

Biomedicines, 2023
Monoclonal antibodies are among the most effective tools for detecting tumor-associated antigens. The U.S. Food and Drug Administration (FDA) has approved more than 36 therapeutic antibodies for developing novel alternative therapies that have ...
Cynthia Rodríguez-Nava, C. Ortuño-Pineda, B. Illades-Aguiar, E. Flores-Alfaro, M. Leyva-Vázquez, I. Parra-Rojas, Oscar del Moral-Hernández, A. Vences-Velázquez, Karen Cortés-Sarabia, L. Alarcón-Romero   +9 more
semanticscholar   +1 more source

Effect of non-repetitive linker on in vitro and in vivo properties of an anti-VEGF scFv

Scientific Reports, 2022
Single chain antibody fragments (scFvs) are favored in diagnostic and therapeutic fields thanks to their small size and the availability of various engineering approaches.
Merve Arslan, Murat Karadag, Ebru Onal, Emine Gelinci, Gulcin Cakan-Akdogan, Sibel Kalyoncu   +5 more
semanticscholar   +1 more source

Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody

Scientific Reports, 2021
Single-chain variable fragments (scFvs) are small-sized artificial constructs composed of the immunoglobulin heavy and light chain variable regions connected by a peptide linker.
R. B. de Aguiar, Tábata de Almeida da Silva, B. Costa, M. Machado, R. Y. Yamada, Camila Braggion, K. R. Perez, M. Mori, Vitor Oliveira, Jane Zveiter de Moraes   +9 more
semanticscholar   +1 more source

Novel Plantibodies Show Promise to Protect Citrus from Greening Disease

Journal of the American Society for Horticultural Science, 2021
Candidatus Liberibacter asiaticus (CLas), the bacteria responsible for citrus greening disease [huanglongbing (HLB)], has become a worldwide threat to citrus (Citrus sp.) production.
Joseph Krystel, Huawei Liu, John Hartung, Ed Stover   +3 more
doaj   +1 more source

Preparation and Directed Evolution of Anti-Ciprofloxacin ScFv for Immunoassay in Animal-Derived Food

Foods, 2021
An immunized mouse phage display scFv library with a capacity of 3.34 × 109 CFU/mL was constructed and used for screening of recombinant anti-ciprofloxacin single-chain antibody for the detection of ciprofloxacin (CIP) in animal-derived food.
Fangyu Wang, Ning Li, Yunshang Zhang, Xuefeng Sun, Man Hu, Yali Zhao, Jianming Fan   +6 more
semanticscholar   +1 more source