Results 31 to 40 of about 52,178 (281)

Engineering Peptide Linkers for scFv Immunosensors [PDF]

Analytical Chemistry, 2008
Using A10B single-chain fragment variable (scFv) as a model system, we demonstrated that the flexibility of scFv linker engineering can be combined with the inherent quick and adaptable characters of surface coupling chemistry (e.g., electrostatic, hydrogen bonding, or covalent attachment) to attach scFv to preformed functionalized self-assembled ...
Zhihong, Shen, Heping, Yan, Ying, Zhang, Raymond L, Mernaugh, Xiangqun, Zeng   +4 more
openaire   +2 more sources

Combinations of Single Chain Variable Fragments From HIV Broadly Neutralizing Antibodies Demonstrate High Potency and Breadth

Frontiers in Immunology, 2021
Broadly neutralizing antibodies (bNAbs) are currently being assessed in clinical trials for their ability to prevent HIV infection. Single chain variable fragments (scFv) of bNAbs have advantages over full antibodies as their smaller size permits ...
Rebecca T. van Dorsten, Rebecca T. van Dorsten, Kshitij Wagh, Penny L. Moore, Penny L. Moore, Penny L. Moore, Lynn Morris, Lynn Morris, Lynn Morris   +8 more
doaj   +1 more source

Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer. [PDF]

, 2017
BackgroundT cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined.
A Molinolo, A Stasi Di, A Thor, B Fisher, BA Irving, BL Levine, C Romeo, CC Kloss, CY Wu, D Moritz, DL Porter, DW Lee, F Guadagni, HM Finney, I Blanco, I Stancovski, J Scholler, JD Wolchok, JL Murray, JN Kochenderfer, JW Greiner, KD Griffith, L Naldini, M Hegde, M Kalos, ME Dudley, MH Finer, MH Kershaw, ML Davila, MR Lander, N Gonzales, NP Restifo, P Hwu, RA Morgan, RE Walker, RF Meredith, RP McGuinness, RT Mitsuyasu, S Guedan, SD Patel, SG Deeks, SL Maude, SV Kashmiri   +42 more
core   +1 more source

A Cassette Vector System for the Rapid Cloning and Production of Bispecific Tetravalent Antibodies

Antibodies, 2012
Bivalent single chain (sc)Fv-Fc antibodies have been used for years as recombinant alternatives of natural immunoglobulins. We have extended this approach to the scFv-Fc-scFv antibody format to obtain tetravalent antigen binding and the possibility to ...
Stefanie Claudia Pohl, Steffi Schwarz, André Frenzel, Thomas Schirrmann   +3 more
doaj   +1 more source

Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update

Molecular Biotechnology, 2020
Single chain variable fragments (scFvs) are generated by joining together the variable heavy and light chain of a monoclonal antibody (mAb) via a peptide linker.
P. Satheeshkumar
semanticscholar   +1 more source

Synthetic Customized scFv Libraries

, 2012
Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or few frameworks.
Gautier, Robin, Pierre, Martineau
openaire   +5 more sources

A Human-Derived Monoclonal Antibody Targeting Extracellular Connexin Domain Selectively Modulates Hemichannel Function [PDF]

, 2019
Connexin hemichannels, which are plasma membrane hexameric channels (connexons) composed of connexin protein protomers, have been implicated in a host of physiological processes and pathological conditions. A number of single point pathological mutations
Buratto, Damiano, Carrer, Andrea, Chiani, Francesco, Kuang, Yuanyuan, Lerner, Richard A, Mammano, Fabio, Nardin, Chiara, Paludetti, Gaetano, Salvatore, Anna Maria, Xu, Liang, Yang, Guang, Ziraldo, Gaia, Zonta, Francesco   +12 more
core   +1 more source

Canine Parvovirus is diagnosed and neutralized by chicken IgY-scFv generated against the virus capsid protein

Veterinary Research, 2020
Canine parvovirus (CPV) can cause acute and highly contagious bloody enteritis in dog. To obtain antibodies against CPV, hens were immunized with virus-like particles (VLP) of CPV-VP2.
Shikun Ge, Longdi Xu, Ben Li, Fagang Zhong, Xiang Liu, Xiaoying Zhang   +5 more
semanticscholar   +1 more source

Hemagglutinin sequence conservation guided stem immunogen design from influenza A H3 subtype [PDF]

, 2015
Seasonal epidemics caused by influenza A (H1 and H3 subtypes) and B viruses are a major global health threat. The traditional, trivalent influenza vaccines have limited efficacy because of rapid antigenic evolution of the circulating viruses.
Citron, Michael, Ferrara, Francesca, Flynn, Jessica A, Liang, Xiaoping, Mallajosyula, Vamsee V A, Temperton, Nigel J., Varadarajan, Raghavan   +6 more
core   +7 more sources

Anti-tumoral potential of a human granulysin-based, CEA-targeted cytolytic immunotoxin

OncoImmunology, 2019
Granulysin is a protein present in the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, with cytolytic activity against microbes and tumors.
Raquel Ibáñez-Pérez, Patricia Guerrero-Ochoa, Sameer Al-Wasaby, Rocío Navarro, Antonio Tapia-Galisteo, Diego De Miguel, Oscar Gonzalo, Blanca Conde, Luis Martínez-Lostao, Ramón Hurtado-Guerrero, Laura Sanz, Alberto Anel   +11 more
doaj   +1 more source