Results 171 to 180 of about 354,238 (282)

Engineering the Bacterial Laccase CotA for Functional Expression and Dye Decolorization Through Site-Directed Mutagenesis. [PDF]

Biology (Basel)
Zhou Z, Yao S, Ying S, Yu M, Song Z, Sun Y, Qian L, Zhang Y.   +7 more
europepmc   +1 more source

Molecular Docking and Site-Directed Mutagenesis of GH49 Family Dextranase for the Preparation of High-Degree Polymerization Isomaltooligosaccharide. [PDF]

Biomolecules, 2023
Wang H, Lin Q, Liu M, Ding W, Weng N, Ni H, Lu J, Lyu M, Wang S.   +8 more
europepmc   +1 more source

Colorectal Cancer Cell's Weapon: RNF32 Engages SPP1+ Macrophages to Foster Liver Metastasis, Targeted by Indole‐3‐Acetic Acid

Advanced Science, EarlyView.
Cytoplasmic RNF32 fuels CRC liver metastasis by degrading GSK3β, which stabilizes β‐catenin and activates Wnt/EMT. Moreover, RNF32 rewires the metastatic niche: it depletes CD8+/CD4+ T and NK cells while recruiting SPP1+ macrophages (which boost tumor stemness via CD44), fibroblasts, and immunosuppressive monocytes to aid colonization.
Hongyu Wang, Shipeng Dai, Yuchen Xie, Pengyu Chen, Yue Chai, Chongyu Wang, Xueying Huang, Xiao Dong, Junfeng Shi, Yongxiang Xia, Xiaofeng Qian, Weiwei Tang, Yichan Zhou   +12 more
wiley   +1 more source

Site-Directed Mutagenesis Using PCR-Mediated Introduction of Silent Mutations

BioTechniques, 1998
Rahul M. Kohli
doaj   +1 more source

Efficient Site-Directed Mutagenesis Mediated by Primer Pairs with 3'-Overhangs. [PDF]

Curr Protoc
Mousavi N, Zhou E, Razavi A, Ebrahimi E, Varela-Castillo P, Yang XJ.   +5 more
europepmc   +1 more source

Effects of Site-Directed Mutagenesis of Cysteine on the Structure of Sip Proteins. [PDF]

Front Microbiol, 2022
Wang L, Ding MY, Wang J, Gao JG, Liu RM, Li HT.   +5 more
europepmc   +1 more source

Multimodal AI‐Driven Identification of Dehydrocostus Lactone as a Potent Renal Fibrosis Attenuator Targeting IQGAP1

Advanced Science, EarlyView.
Renal fibrosis, a hallmark of CKD, lacks effective treatments. Herein, we developed a multimodal AI model (TCM‐SPred) to identify anti‐fibrotic agents and found that dehydrocostus lactone (DCL) targets IQGAP1 to inhibit Wnt signaling, blocking the interaction between IQGAP1 and CCT3, demonstrating potent anti‐fibrotic activity in vitro and in vivo ...
Weijiang Lin, Wenzhuo Xu, Kang Liu, Ping Wang, Zhenzhen Zhu, Wenyu Lu, Zhe Zheng, Xiaoqian Peng, Xunkai Yin, Shulan Mei, An Pan, Jian Liu, Lihong Hu   +12 more
wiley   +1 more source

Investigating the Deoxyribonuclease Activity of CRM197 with Site-Directed Mutagenesis

ACS Omega, 2019
Nathalie Bravo-Bautista, Hieu Hoang, Anusha Joshi, Jennifer Travis, Melissa Wooten, Nathan J. Wymer   +5 more
doaj   +1 more source

Improving enzymatic properties of BlTDH from <i>Bacillus licheniformis</i> through site-directed mutagenesis. [PDF]

Food Chem (Oxf)
Liu X, Gu H, Li H, Chen S, Tang Z, Quan W.   +5 more
europepmc   +1 more source

My remembrances of H.G. Khorana: exploring the mechanism of bacteriorhodopsin with site-directed mutagenesis and FTIR difference spectroscopy. [PDF]

Biophys Rev, 2023
Rothschild KJ.
europepmc   +1 more source