Results 21 to 30 of about 5,655,190 (291)

Short Hairpin RNAs for Strand-Specific Small Interfering RNA Production

Frontiers in Bioengineering and Biotechnology, 2020
RNA interference (RNAi) is an effective mechanism for inhibiting gene expression at the post-transcriptional level. Expression of a messenger RNA (mRNA) can be inhibited by a ∼22-nucleotide (nt) small interfering (si)RNA with the corresponding reverse ...
Peike Sheng, Peike Sheng, Krystal A. Flood, Krystal A. Flood, Mingyi Xie, Mingyi Xie, Mingyi Xie   +6 more
doaj   +1 more source

A small RNA response at DNA ends in Drosophila [PDF]

, 2012
Small RNAs have been implicated in numerous cellular processes, including effects on chromatin structure and the repression of transposons. We describe the generation of a small RNA response at DNA ends in Drosophila that is analogous to the recently ...
Förstemann, Klaus, Böttcher, Romy, Michalik, Katharina M.   +2 more
core   +1 more source

Therapeutic opportunities of small interfering RNA [PDF]

Fundamental & Clinical Pharmacology, 2009
AbstractFormation of small interfering RNA (siRNA) occurs in two steps involving binding of the RNA nucleases to a large double‐stranded RNA (dsRNA) and its cleavage into fragments called siRNA. In the second step, these siRNAs join a multinuclease complex, which degrades the homologous single‐stranded mRNAs.
Bhoomika R, Goyal, Mayur M, Patel, Mithil K, Soni, Shraddha V, Bhadada   +3 more
openaire   +2 more sources

Binding of small interfering RNA molecules is crucial for RNA interference suppressor activity of rice hoja blanca virus NS3 in plants [PDF]

, 2009
The NS3 protein of rice hoja blanca tenuivirus represents a viral suppressor of RNAi that sequesters small interfering (si)RNAs in vitro. To determine whether this siRNA binding property is the critical determinant for the suppressor activity of NS3, an ...
Kaaij, L., Lohuis, H., Hemmes, J.C., Schnettler, E., Goldbach, R.W., Prins, M.W.   +5 more
core   +1 more source

RNA interference is responsible for reduction of transgene expression after Sleeping Beauty transposase mediated somatic integration. [PDF]

, 2012
Integrating non-viral vectors based on transposable elements are widely used for genetically engineering mammalian cells in functional genomics and therapeutic gene transfer.
Anja Ehrhardt, Ehrhardt, Anja, Christina Rauschhuber, Christina Rauschhuber (167226), Anja Ehrhardt (167232), Ehrhardt Anja, Rauschhuber Christina, Rauschhuber, Christina   +7 more
core   +2 more sources

Endogenous small interfering RNAs associated with maize embryonic callus formation. [PDF]

PLoS ONE, 2017
The induction efficiency of maize embryonic callus is highly dependent on the genotype, and only a few lines possess a high capacity for callus formation.
Fei Ge, Xing Huang, Hongmei Hu, Yanling Zhang, Zhaoling Li, Chaoying Zou, Huanwei Peng, Lujiang Li, Shibin Gao, Guangtang Pan, Yaou Shen   +10 more
doaj   +1 more source

The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cells [PDF]

, 2008
The NS3 protein of the tenuivirus rice hoja blanca virus (RHBV) has previously been shown to represent the viral RNA interference (RNAi) suppressor and is active in both plant and insect cells by binding short interfering RNAs (siRNAs) in vitro.
Hemmes, J.C., Schnettler, E., Goldbach, R.W., Prins, M.W.   +3 more
core   +1 more source

Polymers in Small-Interfering RNA Delivery [PDF]

Oligonucleotides, 2011
This review will cover the current strategies that are being adopted to efficiently deliver small interfering RNA using nonviral vectors, including the use of polymers such as polyethylenimine, poly(lactic- co -glycolic acid), polypeptides, chitosan, cyclodextrin, dendrimers, and polymers ...
Singha, K, Namgung, R, Kim, WJ
openaire   +3 more sources

A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity [PDF]

, 2009
The use of chemically synthesized short interfering RNAs (siRNAs) is currently the method of choice to manipulate gene expression in mammalian cell culture, yet improvements of siRNA design is expectably required for successful application in vivo ...
Laursen, Maria B., Bobkov, Georgii V., Herdewijn, Piet, Chattopadhyaya, Jyoti,, Hess, Simone, Van Aerschot, Arthur, Barman, Jharna,, Hansen, Thomas B., Barman, Jharna, Haas, Jens, Zhou, Chuanzheng,, Højland, Torben, Bramsen, Jesper B., Andree, Cordula, Srivastava, Puneet,, Hojland, Torben, Honcharenko, Dmytro,, Meyer, Thomas F., Kjems, Jørgen, Wenska, Malgorzata,, Zhou, Chuanzheng, Babu, B. Ravindra, Smicius, Romualdas, Srivastava, Puneet, Nielsen, Anne F., Odadžić, Dalibor, Müller, Elke, Wengel, Jesper, Bus, Claus, Engels, Joachim W. (Prof. Dr.), Honcharenko, Dmytro, Chattopadhyaya, Jyoti, Abramov, Mikhail, Langkjaer, Niels, Fava, Eugenio, Laursen, Maria B, Mikhailov, Sergey N., Langkjær, Niels, Engels, Joachim W., Wenska, Malgorzata, Zerial, Marino, Van, Aerschot A, Højland, T, Bramsen, JB, Hansen, TB, Laursen, MB, Bus, C, Babu, BR, Langkjaer, N, Nielsen, AF, Abramov, M   +50 more
core   +1 more source

Roles of Small RNAs in Virus-Plant Interactions

Viruses, 2019
Small RNAs (sRNAs), including microRNAs (miRNAs) and short interfering RNAs (siRNAs), are non-coding but powerful RNA molecules of 20−30 nucleotides in length. sRNAs play crucial regulatory roles in diverse plant biological processes.
Baogang Zhang, Wenji Li, Jialin Zhang, Lu Wang, Jianguo Wu   +4 more
doaj   +1 more source