Results 141 to 150 of about 18,841 (183)
Some of the next articles are maybe not open access.
Chromatofocusing of purified placental sphingomyelinase
Biochimie, 1983To establish the profile of the multiple forms of placental lysosomal sphingomyelinase, we have developed in a preliminary step, a new sequence of purification. This protocol has a good yield such that the final preparation is representative of the whole enzyme. Using chromatofocusing, purified sphingomyelinase is resolved into 4 major forms whose pI's
R, Rousson, M T, Vanier, P, Louisot
openaire +2 more sources
Magnesium-dependent sphingomyelinase
Biochemical and Biophysical Research Communications, 1976Abstract An enzyme which requires divalent metals and hydrolyses sphingomyelin to ceramide and phosphorylcholine is present in rat and human brain and practically absent from other organs. The greatest activity is associated with the microsomal fraction.
openaire +2 more sources
Sphingomyelinase of Human Tissues
1978Sphingomyelinase (sphingomyelin phosphodiesterase, E.C. 3.1.4. 12) catalyzes hydrolysis of sphingomyelin to ceramide and phosphorylcholine. A genetic deficiency of this enzyme characterizes at least two distinct clinical forms of Niemann-Pick disease (Crocker’s Type A and Type B (1)).
S, Yamaguchi, K, Suzuki
openaire +2 more sources
Sphingomyelinase of chicken erythrocyte membranes
Archives of Biochemistry and Biophysics, 1975Abstract Most of the chicken erythrocyte's sphingomyelin is hydrolyzed when the chicken red blood cells are incubated in hypotonie solution at 37 °C. Addition of detergents, such as Triton X-100 or Na-cholate, is essential for hydrolysis of external [3H ]sphingomyelin by the erythrocyte membranes.
D, Hirshfeld, A, Loyter
openaire +2 more sources
Role of sphingomyelinases in neurological disorders
Expert Opinion on Therapeutic Targets, 2015Sphingomyelinases, which catalyze the hydrolysis of sphingomyelin to ceramide and phosphorylcholine, are abundant in the brain. These enzymes are a major, rapid source of ceramide production not only during physiological responses to receptor stimulation, but also in neurological disorders.We covered an introduction to sphingomyelinases and its ...
Wei-Yi, Ong +4 more
openaire +2 more sources
Sphingomyelin analogues as inhibitors of sphingomyelinase
Bioorganic & Medicinal Chemistry Letters, 2003To search for neutral sphingomyelinase inhibitors we designed and synthesized hydrolytically stable analogues of sphingomyelin. The novel compounds 8 and 9 which were replaced the phosphodiester moiety of sphingomyelin with the carbamate moiety showed inhibitory activity with an IC(50) value of micro M on neutral sphingomyelinase in rat brain ...
Minoru, Taguchi +10 more
openaire +2 more sources
A spectrophotometric method for determination of sphingomyelinase
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1978A colored derivative of sphingomyelin was synthesized and used as substrate for several sphingomyelinases. The compound is N-omega-trinitrophenyl-aminolaurylsphingosylphosphorylcholine. The rate of hydrolysis of this substrate was compared to that of bovine brain sphingomyelin, labelled with tritium in the choline moiety.
S, Gatt, T, Dinur, Y, Barenholz
openaire +2 more sources
A method for the determination of sphingomyelinase activity
Clinica Chimica Acta, 1975A method is presented for the determination of the activity of sphingomyelinase. It is based on the determination of sphingomyelin before and after enzymatic conversion with the aid of a quantitative paper chromatographic analysis of phospholipids. This method is used for the determination of sphingomyelinase activity in various tissues such as those ...
R E, De Rooij +4 more
openaire +2 more sources
The function of sphingomyelinases in mycobacterial infections
Biological Chemistry, 2018Abstract Tuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the deadliest and most important infectious diseases worldwide. The sphingomyelinase/ceramide system, which has been shown several times to be a crucial factor in the internalization, processing and killing of diverse pathogens, also modulates the pro ...
Wu, Yuqing +2 more
openaire +2 more sources
Acid sphingomyelinase in macrophage apoptosis
Current Opinion in Lipidology, 2004This article presents an overview of the recent progress in understanding metabolic and functional interrelationships of biologically active sphingolipids related to the sphingomyelin signal transduction pathway in relation to the regulation of apoptosis in macrophages.Ceramide generation is an essential, early step in apoptosis in numerous systems ...
Urs P, Steinbrecher +2 more
openaire +2 more sources