Results 1 to 10 of about 2,522 (183)

Concise Synthesis of Functionalized Cyclobutene Analogues for Bioorthogonal Tetrazine Ligation [PDF]

Molecules, 2021
Novel bioorthogonal tools enable the development of new biomedical applications. Here we report the concise synthesis of a series of aryl-functionalized cyclobutene analogues using commercially available starting materials.
Jiayu Sun, Jie Li, Hongbao Sun, Chunling Li, Haoxing Wu   +4 more
doaj   +5 more sources

Recent Advances in the Development of Tetrazine Ligation Tools for Pretargeted Nuclear Imaging [PDF]

Pharmaceuticals, 2022
Tetrazine ligation has gained interest as a bio-orthogonal chemistry tool within the last decade. In nuclear medicine, tetrazine ligation is currently being explored for pretargeted approaches, which have the potential to revolutionize state-of-the-art ...
Rocío García-Vázquez, Umberto Maria Battisti, Matthias M. Herth   +2 more
doaj   +6 more sources

Investigation of Proteome-Tetrazine Reactivity for a Highly Selective Tetrazine Ligation in Live Cells [PDF]

ACS Central Science
Tetrazine has been widely used in bio-orthogonal click chemistry for diverse biological applications due to its short reaction time and excellent bio-orthogonality. For efficient click reaction, the stability of tetrazine in physiological conditions is one of the key issues in biological applications.
Junyoung Park, Juhee Hahm, Junhyeong Yim, Hyelim Lee, Hwan Min Hwang, Soyeon Lee, Ju-Young Park, Arun Velladurai, Jagadeesh Kumar Gangasani, Hana Cho, Hankum Park, Minju Lee, Jeehee Lee, Hyunuk Eom, Woon Ju Song, Sanghee Lee, Eunha Kim, Jongmin Park   +17 more
doaj   +3 more sources

Tunable Synthesis of Hydrogel Microfibers via Interfacial Tetrazine Ligation. [PDF]

Biomacromolecules, 2022
Crosslinked, degradable, and cell-adhesive hydrogel microfibers were synthesized via interfacial polymerization employing tetrazine ligation, an exceptionally fast bioorthogonal reaction between strained trans-cyclooctene (TCO) and s-tetrazine (Tz). A hydrophobic trisTCO crosslinker and homo-difunctional poly(ethylene glycol) (PEG)-based macromers with
George OJ, Song J, Benson JM, Fang Y, Zhang H, Burris DL, Fox JM, Jia X.   +7 more
europepmc   +3 more sources

Intracellular bioorthogonal labeling of glucagon receptor via tetrazine ligation. [PDF]

Bioorg Med Chem, 2021
The third intracellular loop (ICL3) in the cytosolic face of glucagon receptor (GCGR) experiences significant conformational transition during receptor activation. It thus offers an attractive site for the introduction of organic fluorophores in our efforts to construct fluorescence-based GPCR biosensors.
Tian Y, Fang M, Lin Q.
europepmc   +3 more sources

Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems. [PDF]

Chem Sci, 2022
Here, norbornene-tetrazine ligation chemistry is harnessed to control RNA-guided CRISPR systems in vitro and in human cells.
Qi Q, Zhang Y, Xiong W, Liu X, Cui S, Ye X, Zhang K, Tian T, Xiang Zhou.   +8 more
europepmc   +3 more sources

Tetrazine ligation for chemical proteomics. [PDF]

Proteome Sci, 2016
Determining small molecule-target protein interaction is essential for the chemical proteomics. One of the most important keys to explore biological system in chemical proteomics field is finding first-class molecular tools. Chemical probes can provide great spatiotemporal control to elucidate biological functions of proteins as well as for ...
Kang K, Park J, Kim E.
europepmc   +5 more sources

Cellular interactions with hydrogel microfibers synthesized via interfacial tetrazine ligation. [PDF]

Biomaterials, 2018
Fibrous proteins found in the natural extracellular matrix (ECM) function as host substrates for migration and growth of endogenous cells during wound healing and tissue repair processes. Although various fibrous scaffolds have been developed to recapitulate the microstructures of the native ECM, facile synthesis of hydrogel microfibers that are ...
Liu S, Moore AC, Zerdoum AB, Zhang H, Scinto SL, Zhang H, Gong L, Burris DL, Rajasekaran AK, Fox JM, Jia X.   +10 more
europepmc   +4 more sources

Senolysis Enabled by Senescent Cell-Sensitive Bioorthogonal Tetrazine Ligation. [PDF]

Angew Chem Int Ed Engl
AbstractAlthough the clearance of senescent cells has been proven to slow down the aging process and promote anti‐cancer chemotherapy, the development of senolytics remains challenging. Herein, we report a senolytic strategy enabled by senescent cell‐sensitive bioorthogonal tetrazine ligation.
Chang M, Dong Y, Xu H, Cruickshank-Taylor AB, Kozora JS, Behpour B, Wang W.   +6 more
europepmc   +3 more sources

Site-specific bioorthogonal protein labelling by tetrazine ligation using endogenous β-amino acid dienophiles. [PDF]

Nat Chem, 2023
AbstractThe tetrazine ligation is an inverse electron-demand Diels–Alder reaction widely used for bioorthogonal modifications due to its versatility, site specificity and fast reaction kinetics. A major limitation has been the incorporation of dienophiles in biomolecules and organisms, which relies on externally added reagents.
Richter D, Lakis E, Piel J.
europepmc   +4 more sources