Results 21 to 30 of about 2,522 (183)
Small-Molecule Activation of mRNA Translation by Click-to-Release Reaction in Cells. [PDF]
Angew Chem Int Ed EnglA new method to control mRNA activity using bioorthogonal click‐to‐release reactions is presented. The 5′ cap is modified with a trans‐cyclooctene (TCO) that quickly reacts with hydroxyaryl‐tetrazines and efficiently releases the native cap 0. TCO‐capped mRNAs are initially translationally inactive but can be activated with non‐toxic, cell‐permeable ...
Vosman T +5 more
europepmc +3 more sources
Frontiers in Nuclear Medicine, 2022 Alzheimer's disease (AD) is the most common neurodegenerative disease, with an increasing prevalence. Currently, there is no ideal diagnostic molecular imaging agent for diagnosing AD.
Sara Lopes van den Broek +10 more
doaj +1 more source
Rapid oligonucleotide-templated fluorogenic tetrazine ligations [PDF]
Nucleic Acids Research, 2013 Template driven chemical ligation of fluorogenic probes represents a powerful method for DNA and RNA detection and imaging. Unfortunately, previous techniques have been hampered by requiring chemistry with sluggish kinetics and background side reactions.
Šečkutė, Jolita +2 more
openaire +2 more sources
Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing
ACS Chemical Biology, 2022 CRISPR-Cas technology has revolutionized genome editing. Its broad and fast-growing application in biomedical research and therapeutics has led to increased demand for guide RNAs. The synthesis of chemically modified single-guide RNAs (sgRNAs) containing >100 nucleotides remains a bottleneck.
Zexiang Chen +5 more
openaire +3 more sources
Clearing and Masking Agents in Pretargeting Strategies
Pharmaceuticals, 2023 ‘Pretargeting’ led to increased target-to-background ratios of nanomedicines in short timeframes. However, clearing or masking agents are needed to reach the full potential of pretargeted approaches.
Markus Staudt, Matthias M. Herth
doaj +1 more source
Identification of Natural Isonitriles Through Ligation to an Azomethine Imine Probe. [PDF]
ChemistryThe azomethine imine (AMI)–isonitrile (NC) ligation allowed for the chemoselective detection of natural isonitriles in bacteria and fungi. The reactivity‐based screening establishes a new stereogenic center, thereby allowing for facile distinction between chiral and achiral isonitriles. The work also unraveled a unique reactivity of isonitriles bearing
Biedermann MP +6 more
europepmc +2 more sources
IEDDA: An Attractive Bioorthogonal Reaction for Biomedical Applications
Molecules, 2021 The pretargeting strategy has recently emerged in order to overcome the limitations of direct targeting, mainly in the field of radioimmunotherapy (RIT).
Maryana Handula +2 more
doaj +1 more source
Optimization of Direct Aromatic 18F-Labeling of Tetrazines
Molecules, 2022 Radiolabeling of tetrazines has gained increasing attention due to their important role in pretargeted imaging or therapy. The most commonly used radionuclide in PET imaging is fluorine-18.
Ida Vang Andersen +3 more
doaj +1 more source
Synthesis, Fluorine-18 Radiolabeling, and In Vivo PET Imaging of a Hydrophilic Fluorosulfotetrazine
Pharmaceuticals, 2023 The development of 18F-fluorotetrazines, suitable for the radiolabeling of biologics such as proteins and antibodies by IEDDA ligation, represents a major challenge, especially for pre-targeting applications.
Jason Beaufrez +3 more
doaj +1 more source
Development of 18F-Labeled Bispyridyl Tetrazines for In Vivo Pretargeted PET Imaging
Pharmaceuticals, 2022 Pretargeted PET imaging is an emerging and fast-developing method to monitor immuno-oncology strategies. Currently, tetrazine ligation is considered the most promising bioorthogonal reaction for pretargeting in vivo.
Rocío García-Vázquez +7 more
doaj +1 more source