Results 31 to 40 of about 2,522 (183)

Homodimeric Protein–Polymer Conjugates via the Tetrazine–trans-Cyclooctene Ligation [PDF]

Macromolecules, 2015
Tetrazine end-functionalized telechelic polymers were synthesized by controlled radical polymerization (CRP) and employed to generate T4 Lysozyme homodimers. Mutant T4 Lysozyme (V131C), containing a single surface-exposed cysteine, was modified with a protein-reactive trans-cyclooctene (T4L-TCO).
Lorenzo, Maltish M., Decker, Caitlin G., Kahveci, Muhammet U., Paluck, Samantha J., Maynard, Heather D.   +4 more
openaire   +4 more sources

Evaluation of [⁶⁴Cu]Cu-NOTA-PEG₇-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [¹¹¹In]In-DOTA-PEG₁₁-BisPy-Tz

Molecules, 2021
Pretargeted nuclear imaging for the diagnosis of various cancers is an emerging and fast developing field. The tetrazine ligation is currently considered the most promising reaction in this respect. Monoclonal antibodies are often the preferred choice as
Christian B. M. Poulie, Jesper T. Jørgensen, Vladimir Shalgunov, Georgios Kougioumtzoglou, Troels Elmer Jeppesen, Andreas Kjaer, Matthias M. Herth   +6 more
doaj   +1 more source

Discovery of new mutually orthogonal bioorthogonal cycloaddition pairs through computational screening. [PDF]

, 2015
Density functional theory (DFT) calculations and experiments in tandem led to discoveries of new reactivities and selectivities involving bioorthogonal sydnone cycloadditions.
Houk, KN, Liang, Yong, Murphy, Jennifer M, Narayanam, Maruthi Kumar   +3 more
core   +1 more source

Controlled Orientation of Active Sites in a Nanostructured Multienzyme Complex [PDF]

, 2016
Multistep cascade reactions in nature maximize reaction efficiency by co-assembling related enzymes. Such organization facilitates the processing of intermediates by downstream enzymes.
A Sachdeva, A Weckbecker, BM Hutchins, CC Roberts, CH Kim, CJ Delebecque, D Guan, DE Torres Pazmino, EM Brustad, H Choe, H Nanba, H Pettersson, HS Seo, J Fu, J Fu, J-L Lin, JA Johnson, JE Dueber, JW Chin, M Castellana, M Leibundgut, M Slatner, MG Hoesl, MH Seo, MJ Lajoie, ML Blackman, NJ Agard, O Idan, P Bauler, P Brunker, P Bubner, R Narayanaswamy, RJ Conrado, S An, S Schoffelen, SI Lim, SI Lim, SI Lim, SI Lim, SR Bond, TM Schmeing, TS Moon, Y Kim, Y Zhang   +43 more
core   +7 more sources

Evaluation of a ⁶⁸Ga-Labeled DOTA-Tetrazine as a PET Alternative to ¹¹¹In-SPECT Pretargeted Imaging

Molecules, 2020
The bioorthogonal reaction between a tetrazine and strained trans-cyclooctene (TCO) has garnered success in pretargeted imaging. This reaction was first validated in nuclear imaging using an 111In-labeled 1,4,7,10-tetraazacyclododecane-1,4,7,10 ...
Patricia E. Edem, Jesper T. Jørgensen, Kamilla Nørregaard, Rafaella Rossin, Abdolreza Yazdani, John F. Valliant, Marc Robillard, Matthias M. Herth, Andreas Kjaer   +8 more
doaj   +1 more source

Tailoring Chimeric Ligands for Studying and Biasing ErbB Receptor Family Interactions [PDF]

, 2013
Described is the development and application of a versatile semisynthetic strategy, based on a combination of sortase-mediated coupling and tetrazine ligation chemistry, which can be exploited for the efficient incorporation of tunable functionality into
Amin, Baselga, Bundy, Butt, Chen, Ciardiello, Citri, Devaraj, Engelman, Hudak, Inagaki, Iyer, Jay, Jay, Jeppesen, Karver, Kumar, Lee, Lemmon, Linggi, Lu, Mammen, Marblestone, Martino, Nagel, Nandagopal, Olayioye, Popp, Proft, Reddy, Riese, Rodrigues, Sainlos, Scheck, Schellinger, Tanaka, Wiley, Wingens, Witte, Wu   +39 more
core   +1 more source

Injectable bioorthogonal hydrogel (BIOGEL) accelerates tissue regeneration in degenerated intervertebral discs

Bioactive Materials, 2023
Intervertebral disc (IVD) degeneration is a leading cause of back pain and precursor to more severe conditions, including disc herniation and spinal stenosis.
Jeffrey Luo, Anjani Darai, Thanapat Pongkulapa, Brian Conley, Letao Yang, Inbo Han, Ki-Bum Lee   +6 more
doaj   +1 more source

Site-selective protein-modification chemistry for basic biology and drug development. [PDF]

, 2015
Nature has produced intricate machinery to covalently diversify the structure of proteins after their synthesis in the ribosome. In an attempt to mimic nature, chemists have developed a large set of reactions that enable post-expression modification of ...
A Perols, A Sachdeva, AJ Keefe, B Fierz, B-Q Shen, BM Zeglis, C Chatterjee, C Goodman, CD Spicer, CT Walsh, D Schumacher, DP Nguyen, E Arbely, EM Pelegri-O'Day, EM Sletten, F Casadio, F Li, F Tian, Filipa P. da Cruz, G Pasut, GJL Bernardes, Gonçalo J. L. Bernardes, H Cho, H Neumann, HF Gaertner, HP Hemantha, J Li, J Li, J Madrzak, J Xie, J-M Kee, JI MacDonald, JL Seitchik, JM Chalker, JM Chalker, JM Chalker, JM Gilmore, JR Junutula, JY Axup, K Lang, K Lang, K Wang, KJ Hamblett, KP Chooi, L Davis, LN Tumey, M Rashidian, M Steiner, M Yang, MD Simon, N Stephanopoulos, N Toda, Nikolaus Krall, NS Beckley, Omar Boutureira, P Agarwal, P Dawson, P Wang, PM Drake, PMSD Cal, R Huang, R Rossin, R Serwa, RM Versteegen, RP Lyon, RVJ Chari, RVJ Chari, S Arur, S Tyagi, S Virdee, S Virdee, S Zheng, SI van Kasteren, SNS Alconcel, T Burckstummer, T Schneider, TS Elliott, TW Muir, W Liu   +78 more
core   +1 more source

Alkene–Tetrazine Ligation for Imaging Cellular DNA

Angewandte Chemie, 2014
Abstract5‐Vinyl‐2′‐deoxyuridine (VdU) is the first reported metabolic probe for cellular DNA synthesis that can be visualized by using an inverse electron demand Diels–Alder reaction with a fluorescent tetrazine. VdU is incorporated by endogenous enzymes into the genomes of replicating cells, where it exhibits reduced genotoxicity compared to 5‐ethynyl‐
Rieder Ulrike, Luedtke Nathan W.
openaire   +3 more sources

Pretargeted Imaging beyond the Blood–Brain Barrier—Utopia or Feasible?

Pharmaceuticals, 2022
Pretargeting is a promising nuclear imaging technique that allows for the usage of antibodies (Abs) with enhanced imaging contrast and reduced patient radiation burden. It is based on bioorthogonal chemistry with the tetrazine ligation—a reaction between
Sara Lopes van den Broek, Vladimir Shalgunov, Rocío García Vázquez, Natalie Beschorner, Natasha S. R. Bidesi, Maiken Nedergaard, Gitte M. Knudsen, Dag Sehlin, Stina Syvänen, Matthias M. Herth   +9 more
doaj   +1 more source