Results 51 to 60 of about 2,522 (183)
Expanding room for tetrazine ligations in the in vivo chemistry toolbox [PDF]
Current Opinion in Chemical Biology, 2013 There is tremendous interest in developing and refining methods to predictably perform chemical reactions within the framework of living systems. Here we review recent advances in applying tetrazine cycloadditions to live cell and in vivo chemistry. We highlight new syntheses of the tetrazine and dienophile precursors useful for in vivo studies.
Jolita, Sečkutė, Neal K, Devaraj
openaire +2 more sources
Molecules, 2020 Fluorine-18 is the most widely used positron emission tomography (PET) radionuclide currently in clinical application, due to its optimal nuclear properties. The synthesis of 18F-labeled radiotracers often requires harsh reaction conditions, limiting the
Sofia Otaru +5 more
doaj +1 more source
Enzymatic Cross-Linking of Dynamic Thiol-Norbornene Click Hydrogels [PDF]
, 2019 Enzyme-mediated in situ forming hydrogels are attractive for many biomedical applications because gelation afforded by enzymatic reactions can be readily controlled not only by tuning macromer compositions, but also by adjusting enzyme kinetics.
Hudson, Britney N. +3 more
core +2 more sources
Efficient synthesis of single-chain polymer nanoparticles via amide formation [PDF]
, 2015 This is an open access article distributed under the Creative Commons Attribution License.Single-chain technology (SCT) allows the transformation of individual polymer chains to folded/collapsed unimolecular soft nanoparticles. In this work we contribute
Pomposo, José A. +1 more
core +4 more sources
Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for Single-Molecule Imaging on Living Cells [PDF]
, 2012 We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition ...
Alice Y. Ting +38 more
core +1 more source
Tetrazine Ligation: Fast Bioconjugation Based on Inverse-Electron-Demand Diels−Alder Reactivity [PDF]
Journal of the American Chemical Society, 2008 Described is a bioorthogonal reaction that proceeds with unusually fast reaction rates without need for catalysis: the cycloaddition of s-tetrazine and trans-cyclooctene derivatives. The reactions tolerate a broad range of functionality and proceed in high yield in organic solvents, water, cell media, or cell lysate.
Melissa L, Blackman +2 more
openaire +2 more sources
Angewandte Chemie, Volume 138, Issue 17, 20 April 2026.The cupula is a membrane within the vestibular organ that senses rotatory accelerations of the head. Metabolic glycoengineering in combination with bioorthogonal labeling demonstrates that in adult zebrafish – being a model for the human inner ear – this membrane is constantly renewed.
Hans Scherer +4 more
wiley +2 more sources
Advances in chemical protein modification. [PDF]
, 2015 Chemical protein modification has emerged as an invaluable tool for the development of modified proteins. The complementary use of both genetic and chemical methods has provided a large toolbox that allows the preparation of almost unlimited protein ...
Bernardes, Gonçalo JL, Boutureira, Omar
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Tetrazine–trans-cyclooctene ligation for the rapid construction of 18F labeled probes [PDF]
Chemical Communications, 2010 A radiolabeling method for bioconjugation based on the Diels-Alder reaction between 3,6-diaryl-s-tetrazines and an (18)F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.
Li, Zibo +6 more
openaire +3 more sources
Metabolic Labeling and Imaging of Cellular RNA via Bioorthogonal Cyclopropene−Tetrazine Ligation [PDF]
CCS Chemistry, 2020 RNA labeling is vital for the study of an RNA structure, cellular distribution, localization, and metabolism.
Zhiyong He +6 more
openaire +1 more source