Results 21 to 30 of about 4,159 (191)
Deep sequencing of yeast and mouse tRNAs and tRNA fragments using OTTR [PDF]
eLifeAmong the major classes of RNAs in the cell, tRNAs remain the most difficult to characterize via deep sequencing approaches, as tRNA structure and nucleotide modifications can each interfere with cDNA synthesis by commonly used reverse transcriptases ...
Hans Tobias Gustafsson +8 more
doaj +2 more sources
Context-specific Angiogenin-mediated tRNA fragments (tDRs) biogenesis shapes the mitochondrial stress response [PDF]
Redox BiologyTransfer RNA-derived small RNAs (tDRs) are emerging regulators of cellular stress response, yet their biogenesis and activities during mitochondrial dysfunction remain poorly understood.
Shadi Al-Mesitef +7 more
doaj +2 more sources
5' tRNA halves are highly expressed in the primate hippocampus and might sequence-specifically regulate gene expression. [PDF]
RNA, 2020 Fragments of mature tRNAs have long been considered as mere degradation products without physiological function. However, recent reports show that tRNA-derived small RNAs (tsRNAs) play prominent roles in diverse cellular processes across a wide spectrum of species.
Jehn J +9 more
europepmc +6 more sources
Processing by RNase 1 forms tRNA halves and distinct Y RNA fragments in the extracellular environment. [PDF]
Nucleic Acids Res, 2020 Abstract Extracellular RNAs participate in intercellular communication, and are being studied as promising minimally invasive diagnostic markers. Several studies in recent years showed that tRNA halves and distinct Y RNA fragments are abundant in the extracellular space, including in biofluids.
Nechooshtan G +3 more
europepmc +4 more sources
Identification of aberrant tRNA-halves expression patterns in clear cell renal cell carcinoma. [PDF]
Sci Rep, 2016 AbstractSmall non-coding RNAs (sncRNA; <200 nt) regulate various cellular processes and modify gene expression. Under nutritional, biological or physiochemical stress some mature sncRNAs (e.g. tRNAs) are cleaved into halves (30–50 nt) and smaller fragments (18–22 nt); the significance and functional role of these tRNA fragments is unknown, but their
Nientiedt M +5 more
europepmc +4 more sources
Stable tRNA halves can be sorted into extracellular vesicles and delivered to recipient cells in a concentration-dependent manner. [PDF]
RNA Biol, 2020 Extracellular vesicles (EVs) are cell-derived nanoparticles that act as natural carriers of nucleic acids between cells. They offer advantages as delivery vehicles for therapeutic nucleic acids such as small RNAs. Loading of desired nucleic acids into EVs can be achieved by electroporation or transfection once purified.
Gámbaro F +10 more
europepmc +4 more sources
Emerging roles of tRNA-derived fragments in cancer
Molecular Cancer, 2023 tRNA-derived fragments (tRFs) are an emerging category of small non-coding RNAs that are generated from cleavage of mature tRNAs or tRNA precursors. The advance in high-throughput sequencing has contributed to the identification of increasing number of ...
Min Fu +7 more
doaj +1 more source
Neural Regeneration Research, 2020 Over the past decades, tRNA was found to be a rich hub of RNA modifications such as 1-methyladenosine and 5-methycytosine modifications and others, holding more than half of all modifications occurring in RNA molecules.
Sherif Rashad +2 more
doaj +1 more source
Parasites & Vectors, 2021 Background Small non-coding RNAs play critical regulatory roles in post-transcription. However, their characteristics in Trichomonas vaginalis, the causative agent of human sexually transmitted trichomoniasis, still remain to be determined. Methods Small
Zhen-Sheng Wang +8 more
doaj +1 more source
Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples. [PDF]
Sci Rep, 2016 AbstractThe ongoing ascent of sequencing technologies has enabled researchers to gain unprecedented insights into the RNA content of biological samples. MiRNAs, a class of small non-coding RNAs, play a pivotal role in regulating gene expression. The discovery that miRNAs are stably present in circulation has spiked interest in their potential use as ...
Van Goethem A +10 more
europepmc +5 more sources