Results 51 to 60 of about 198,753 (295)

Metabolomics guides rational development of a simplified cell culture medium for drug screening against Trypanosoma brucei [PDF]

, 2013
n vitro culture methods underpin many experimental approaches to biology and drug discovery. The modification of established cell culture methods to make them more biologically relevant or to optimize growth is traditionally a laborious task.
Alsford, Alsford, Baltz, Barrett, Berger, Berger, Bitonti, Bowling, Bradley, Breitling, Brun, Brunda Nijagal, Creek, Creek, Creek, Darren J. Creek, de Koning, Dong-Hyun Kim, Drexler, Duszenko, Duszenko, Federico Rojas, Geary, Haanstra, Hasne, Hirumi, Hirumi, Hirumi, Jacobs, Keith R. Matthews, Kuettel, Kwon, Lanteri, Lanteri, Mashego, Michael P. Barrett, Mäser, Räz, Saunders, Scalbert, Scheltema, Scheltema, Seed, Sienkiewicz, Smith, Sumner, Sykes, t'Kindt, Tautenhahn, Vincent, Wang, Ward, Wilson, Winder, Wishart, Wu, Zaboikin, Zang, Zhang   +58 more
core   +2 more sources

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency

Cell Death Discovery, 2021
Tryptophan metabolism is an essential regulator of tumor immune evasion. However, the effect of tryptophan metabolism on cancer cells remains largely unknown.
Mingqing Cui, Dan Liu, Wujun Xiong, Yugang Wang, Jun Mi   +4 more
doaj   +1 more source

Effects of dietary long-chain polyunsaturated fatty acids on plasma amino acids and indices of protein metabolism in infants: Results from a randomized clinical trial [PDF]

, 1998
Background/Aim: Previous studies in vitro and in animals in vivo found that alpha-linolenic acid (C18:3 omega 3) may enhance oxidative damage of essential amino acids.
Burus, I., Decsi, Tamás, Koletzko, Berthold   +2 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Exploring the interplay between running exercises, microbial diversity, and tryptophan metabolism along the microbiota-gut-brain axis

Frontiers in Microbiology
The emergent recognition of the gut-brain axis connection has shed light on the role of the microbiota in modulating the gut-brain axis’s functions. Several microbial metabolites, such as serotonin, kynurenine, tryptamine, indole, and their derivatives ...
Alejandra Vazquez-Medina, Nicole Rodriguez-Trujillo, Kiara Ayuso-Rodriguez, Fabiana Marini-Martinez, Roberto Angeli-Morales, Gerardo Caussade-Silvestrini, Filipa Godoy-Vitorino, Nataliya Chorna   +7 more
doaj   +1 more source

Mapping an atlas of tissue-specific drosophila melanogaster metabolomes by high resolution mass spectrometry [PDF]

, 2013
Metabolomics can provide exciting insights into organismal function, but most work on simple models has focussed on the whole organism metabolome, so missing the contributions of individual tissues.
A Kamleh, A Malmendal, A Malmendal, AA Jackson, C Jeulin, David G. Watson, DG Watson, DJ Creek, Dominika Korzekwa, DT Sullivan, G Di Paolo, G Fraenkel, GJ Blomquist, GX Quan, H Colinet, J Andrews, J Overgaard, Jacob Guy Bundy, JD Feala, JE Treherne, JL Hubby, JS Park, Julian A. T. Dow, KS Pedersen, L Coquin, L Pan, L Zheng, M Carvalho, M Parisi, M Rera, MA Kamleh, MA Kamleh, MF Wolfner, MM Green, Mohammed Al Bratty, MS Elias, N Vereshchagina, NE Braverman, P Niewiadomska, PA Robitaille, PS Shekhawat, R Tearle, S Buch, S Reisse, V Kostal, Venkateswara R. Chintapalli, VR Chintapalli, VR Chintapalli   +47 more
core   +3 more sources

Signaling pathways linking behavior to neurogenesis in healthy brain and disease [PDF]

, 2020
Self-repair of the adult brain is limited – most diseases lack effective therapy. In order to better understand how a regenerative response can be achieved, studying mechanisms shaping the neurogenic niche, from environmental factors to intrinsic ...
Klempin, Friederike
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

A Defect in Tryptophan Metabolism [PDF]

Pediatric Research, 1976
Oral tryptophan loading tests were performed in a patient with photosensitive pellagra-like skin rash and cerebellar ataxia but without hyperaminoaciduria. Plasma tryptophan concentrations after loading were similar in the patient and control subjects.
P W, Wong, P, Forman, B, Tabahoff, P, Justice   +3 more
openaire   +2 more sources