Results 11 to 20 of about 4,897 (216)

Identification of a potential general acid/base in the reversible phosphoryl transfer reactions catalyzed by tyrosine recombinases: Flp H305. [PDF]

Chem Biol, 2007
Flp provides a unique opportunity to apply the tools of chemical biology to phosphoryl transfer reactions. Flp and other tyrosine recombinases catalyze site-specific DNA rearrangements via a phosphotyrosine intermediate. Unlike most related enzymes, Flp's nucleophilic tyrosine derives from a different protomer than the remainder of its active site ...
Whiteson KL, Chen Y, Chopra N, Raymond AC, Rice PA.   +4 more
europepmc   +3 more sources

Mechanistic modelling of tyrosine recombination reveals key parameters determining the performance of a CAR T cell switching circuit

Engineering Biology, 2020
Inducible genetic switches based on tyrosine recombinase-based DNA excision are a promising platform for the regulation and control of chimeric antigen receptor (CAR) T cell activity in cancer immunotherapy. These switches exploit the increased stability
Jack E. Bowyer, Deboki Chakravarti, Wilson W. Wong, Declan G. Bates   +3 more
doaj   +1 more source

A New Group of Tyrosine Recombinase-Encoding Retrotransposons [PDF]

Molecular Biology and Evolution, 2004
A wide variety of novel tyrosine recombinase (YR)-encoding retrotransposons were identified using data emerging from the various eukaryotic genome sequencing projects. Although many of these elements are clearly members of the previously described DIRS group of YR retrotransposons, a substantial number, including elements from a variety of fungi and ...
Timothy J D, Goodwin, Russell T M, Poulter   +1 more
openaire   +2 more sources

c‐Rel–dependent Chk2 signaling regulates the DNA damage response limiting hepatocarcinogenesis

Hepatology, EarlyView., 2022
In response to genotoxic injury, c‐Rel upregulates ATM‐Chk2‐p53 pathway DNA damage proteins to limiting hepatocarcinogenesis. Abstract Background and Aims Hepatocellular carcinoma (HCC) is a leading cause of cancer‐related death. The NF‐κB transcription factor family subunit c‐Rel is typically protumorigenic; however, it has recently been reported as a
Jack Leslie, Jill E. Hunter, Amy Collins, Amelia Rushton, Lauren G. Russell, Erik Ramon‐Gil, Maja Laszczewska, Misti McCain, Marco Y. W. Zaki, Amber Knox, Yixin Seow, Laura Sabater, Daniel Geh, Neil D. Perkins, Helen L. Reeves, Dina Tiniakos, Derek A. Mann, Fiona Oakley   +17 more
wiley   +1 more source

Tyroviruses are a new group of temperate phages that infect Bacillus species in soil environments worldwide

BMC Genomics, 2022
Background Bacteriophages are widely considered to be highly abundant and genetically diverse, with their role in the evolution and virulence of many pathogens becoming increasingly clear.
Steven Batinovic, Cassandra R. Stanton, Daniel T. F. Rice, Brittany Rowe, Michael Beer, Steve Petrovski   +5 more
doaj   +1 more source

The XerC recombinase ofProteus mirabilis: characterization and interaction with other tyrosine recombinases [PDF]

FEMS Microbiology Letters, 2003
XerC and XerD are two site-specific recombinases, which act on different sites to maintain replicons in a monomeric state. This system, which was first discovered and studied in Escherichia coli, is present in several species including Proteus mirabilis, where the XerD recombinase was previously characterized by our laboratory. In this paper, we report
Manuela, Villion, George, Szatmari
openaire   +2 more sources

The dif/Xer recombination systems in proteobacteria. [PDF]

PLoS ONE, 2009
In E. coli, 10 to 15% of growing bacteria produce dimeric chromosomes during DNA replication. These dimers are resolved by XerC and XerD, two tyrosine recombinases that target the 28-nucleotide motif (dif) associated with the chromosome's replication ...
Christophe Carnoy, Claude-Alain Roten
doaj   +1 more source

Challenging a Paradigm: the Role of DNA Homology in Tyrosine Recombinase Reactions [PDF]

Microbiology and Molecular Biology Reviews, 2009
SUMMARYA classical feature of the tyrosine recombinase family of proteins catalyzing site-specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp recombinases, is the ability to recombine substrates sharing very limited DNA sequence identity.
Lara, Rajeev, Karolina, Malanowska, Jeffrey F, Gardner   +2 more
openaire   +2 more sources

Resolution of Mismatched Overlap Holliday Junction Intermediates by the Tyrosine Recombinase IntDOT [PDF]

Journal of Bacteriology, 2017
ABSTRACT CTnDOT is an integrated conjugative element found in Bacteroides species. CTnDOT contains and transfers antibiotic resistance genes. The element integrates into and excises from the host chromosome via a Holliday junction (HJ) intermediate as part of a site-specific recombination mechanism.
Kenneth, Ringwald, Sumiko, Yoneji, Jeffrey, Gardner   +2 more
openaire   +2 more sources

The carboxy-terminal αN helix of the archaeal XerA tyrosine recombinase is a molecular switch to control site-specific recombination.

PLoS ONE, 2013
Tyrosine recombinases are conserved in the three kingdoms of life. Here we present the first crystal structure of a full-length archaeal tyrosine recombinase, XerA from Pyrococcus abyssi, at 3.0 Å resolution.
Marie-Claude Serre, Toufic El Arnaout, Mark A Brooks, Dominique Durand, Johnny Lisboa, Noureddine Lazar, Bertrand Raynal, Herman van Tilbeurgh, Sophie Quevillon-Cheruel   +8 more
doaj   +1 more source