Results 21 to 30 of about 4,897 (216)

Evidence for a Xer/dif system for chromosome resolution in archaea. [PDF]

PLoS Genetics, 2010
Homologous recombination events between circular chromosomes, occurring during or after replication, can generate dimers that need to be converted to monomers prior to their segregation at cell division.
Diego Cortez, Sophie Quevillon-Cheruel, Simonetta Gribaldo, Nicole Desnoues, Guennadi Sezonov, Patrick Forterre, Marie-Claude Serre   +6 more
doaj   +1 more source

Simultaneous Multiplex Genome Engineering via Accelerated Natural Transformation in Bacillus subtilis

Frontiers in Microbiology, 2021
Multiplex engineering at the scale of whole genomes has become increasingly important for synthetic biology and biotechnology applications. Although several methods have been reported for engineering microbe genomes, their use is limited by their complex
Aihua Deng, Zhaopeng Sun, Tiantian Wang, Tiantian Wang, Di Cui, Lai Li, Lai Li, Shuwen Liu, Shuwen Liu, Fei Huang, Tingyi Wen, Tingyi Wen, Tingyi Wen   +12 more
doaj   +1 more source

Making serine integrases work for us [PDF]

, 2017
DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements.
Stark, W. Marshall
core   +1 more source

Identification and Characterization of a Phase-Variable Element That Regulates the Autotransporter UpaE in Uropathogenic Escherichia coli

mBio, 2018
Uropathogenic Escherichia coli (UPEC) is the most common etiologic agent of uncomplicated urinary tract infection (UTI). An important mechanism of gene regulation in UPEC is phase variation that involves inversion of a promoter-containing DNA element via
E. J. Battaglioli, K. G. K. Goh, T. S. Atruktsang, K. Schwartz, M. A. Schembri, R. A. Welch   +5 more
doaj   +1 more source

Nearest-neighbor amino acids of specificity-determining residues influence the activity of engineered Cre-type recombinases

Scientific Reports, 2020
The tyrosine-type site-specific DNA recombinase Cre recombines its target site, loxP, with high activity and specificity without cross-recombining the target sites of highly related recombinases. Understanding how Cre achieves this precision is key to be
Anjali Soni, Martina Augsburg, Frank Buchholz, M. Teresa Pisabarro   +3 more
doaj   +1 more source

Tyrosine Recombinase Retrotransposons and Transposons

Microbiology Spectrum, 2015
ABSTRACT Retrotransposons carrying tyrosine recombinases (YR) are widespread in eukaryotes. The first described tyrosine recombinase mobile element, DIRS1, is a retroelement from the slime mold Dictyostelium discoideum . The YR elements are bordered by terminal repeats related to their replication via free
Russell T M, Poulter, Margi I, Butler
openaire   +2 more sources

Promoter Orientation of Prokaryotic Phase-variable Genes by PCR

Bio-Protocol, 2012
One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR.
Stacey Bateman, Patrick Seed
doaj   +1 more source

FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae. [PDF]

PLoS Genetics, 2008
Unlike most bacteria, Vibrio cholerae harbors two distinct, nonhomologous circular chromosomes (chromosome I and II). Many features of chromosome II are plasmid-like, which raised questions concerning its chromosomal nature.
Marie-Eve Val, Sean P Kennedy, Meriem El Karoui, Laetitia Bonné, Fabien Chevalier, François-Xavier Barre   +5 more
doaj   +1 more source

Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending [PDF]

, 2016
Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Adams, Afonine, Aihara, Arnold, Aussel, Barre, Barre, Biswas, Blakely, Blakely, Blakely, Britton, Carnoy, Chen, Cortez, Das, Debowski, Debowski, Diagne, Dong, Emsley, Ennifar, Fischer, Gopaul, Gopaul, Grainge, Grindley, Guo, Guo, Hendricks, Holliday, Jayaram, Jo, Kabsch, Karaca, Keller, Kelley, Kim, Krissinel, Kuempel, Le Bourgeois, Lee, Leroux, Li, Luscombe, May, McCoy, Midonet, Nagy, Nolivos, Nunes-Düby, Page, Pargellis, Pettersen, Pérals, Rother, Serre, Steiner, Subramanya, Terwilliger, Turan, Val, Van Duyne, Van Duyne, van Zundert, Vonrhein, Yang, Yang, Yates, Yates, Zawadzki, Zheng   +71 more
core   +4 more sources

Tyrosine phosphorylation stimulates activity of human RAD51 recombinase through altered nucleoprotein filament dynamics [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Homologous recombination provides the most accurate means to repair genotoxic DNA lesions. It depends on the assembly of the RAD51 DNA strand exchange protein into a dynamic nucleoprotein filament. The c-Abl tyrosine kinase and its oncogenic counterpart BCR-ABL control RAD51 by phosphorylating tyrosine residues 54 and 315.
Shyamal Subramanyam, Mohammed Ismail, Ipshita Bhattacharya, Maria Spies   +3 more
openaire   +2 more sources