Results 71 to 80 of about 4,897 (216)

Control of serine integrase recombination directionality by fusion with the directionality factor [PDF]

, 2017
Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL ...
Akopian, Arlene L. McPherson, Arnold, Bonnet, Bonnet, Brophy, Brown, Colloms, Fan, Farruggio, Femi J. Olorunniji, Fogg, Ghosh, Khaleel, Kilbride, Kuhstoss, Ladurner, Lewis, Mandali, Maranhao, Margaret C.M. Smith, Moore, Olorunniji, Olorunniji, Olorunniji, Olorunniji, Olorunniji, Pokhilko, Prorocic, Proudfoot, Roquet, Rowley, Rutherford, Rutherford, Sean D. Colloms, Smith, Susan J. Rosser, Van Duyne, W. Marshall Stark, Weinberg, Xu, Yang, Zhang, Zhang   +43 more
core   +3 more sources

Requirements for Site-Specific Recombination in the Tyrosine-Family Recombinase Active Site [PDF]

Biophysical Journal, 2014
Tyrosine recombinase (YR) shares the same active site and catalyzes the DNA rearrangement with identical phosphoryl transfer chemistry to topoisomerases IB (TopIBs). In order to find out the function of each residue within conserved catalytic center and what factor dictates the strikingly different kinetic characteristics, in cis for Cre and λ Int and ...
openaire   +1 more source

Chaperone‐Mediated Autophagic Degradation of USP9X in Macrophages Exacerbates Postmyocardial Infarction Inflammation and Cardiac Dysfunction

Advanced Science, EarlyView.
This study demonstrates that inflammatory stimuli induce the acetylation‐triggered, chaperone‐mediated autophagic degradation of ubiquitin‐specific peptidase 9 X‐linked (USP9X) in macrophages. USP9X acts as a macrophage “inflammation switch” after myocardial infarction (MI). USP9X loss destabilizes tumor necrosis factor receptor‐associated factor (TRAF)
Biqing Wang, Xiangheng Cai, Mengqi Li, Xue Liu, Junhui Xue, Ye Liu, Ding Ai, Xinyang Hu   +7 more
wiley   +1 more source

In vitro site‐specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum [PDF]

Genes to Cells, 2017
In organisms with circular chromosomes, such as bacteria and archaea, an odd number of homologous recombination events can generate a chromosome dimer. Such chromosome dimers cannot be segregated unless they are converted to monomers before cell division.
Minji Jo, Yasuto Murayama, Yasuhiro Tsutsui, Hiroshi Iwasaki   +3 more
openaire   +2 more sources

Cars2‐Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2

Advanced Science, EarlyView.
We demonstrate that Cars2, a cysteine catabolic enzyme in mouse iBAT, is critical for cold tolerance and brown adipocyte differentiation. Through its CPERS activity, Cars2 produces CysSSH/H2S to induce EBF2 persulfidation, promoting its interaction with PPARγ and BRG1 to enhance thermogenic gene expression.
Xin Peng, Hongyong Song, Yin Long, Kaimin Wu, Yinkun Fu, Wenkai Zhou, Wenyan Li, Zhe Shi, Xu‐Yun Zhao   +8 more
wiley   +1 more source

Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes

BMC Genomics, 2011
Background During the replication process of bacteria with circular chromosomes, an odd number of homologous recombination events results in concatenated dimer chromosomes that cannot be partitioned into daughter cells.
Arakawa Kazuharu, Kono Nobuaki, Tomita Masaru   +2 more
doaj   +1 more source

Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae [PDF]

, 2016
Background: Phage-encoded serine integrases, such as ϕC31 integrase, are widely used for genome engineering but have not been optimized for use in Saccharomyces cerevisiae although this organism is a widely used organism in biotechnology. Results: The
Brown, William, Xu, Zhengyao
core   +2 more sources

The 9th International RASopathies Symposium

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT The RASopathies are a group of congenital disorders with overlapping clinical manifestations that are caused by pathogenic germline or early somatic variants that result in the hyperactivation of the RAS/mitogen‐activated protein kinase (MAPK) signaling pathway.
Pau Castel, Lisa Schoyer, Beth Stronach, Raya Bogdanova, Anton M. Bennett, Jaishri Blakeley, Miriam Bornhorst, Tammy Bowers, Saskia M. Brachmann, Emma Burkitt‐Wright, Kathryn Chatfield, Alessandro De Luca, Khalil El‐Chammas, Abdul Elkadri, John E. Fortunato, Bruce D. Gelb, Anne Goriely, Karen Gripp, Kassidy Grover, Lindsay Homan, Kenneth A. Kern, Maija Kiuru, Charles (Chuck) Lawson, Yong‐Seok Lee, Frank McCormick, Gina Ney, Cristina Nuevo‐Tapioles, Sara Pardej, Elizabeth I. Pierpont, Julia Plank, Nancy Ratner, Katherine A. Rauen, J. Elliott Robinson, Les Rogers, Sarah E. Sheppard, Keir Shiels, David Stevenson, Dagmar Tiemens, Matthew Traylor, K. Nicole Weaver, Marielle Yohe, Tamar Green   +41 more
wiley   +1 more source

Four Chromosomal Type IV Secretion Systems in Helicobacter pylori: Composition, Structure and Function

Frontiers in Microbiology, 2020
The pathogenic bacterium Helicobacter pylori is genetically highly diverse and a major risk factor for the development of peptic ulcer disease and gastric adenocarcinoma in humans. During evolution, H.
Wolfgang Fischer, Nicole Tegtmeyer, Kerstin Stingl, Steffen Backert   +3 more
doaj   +1 more source

topIb, a phylogenetic hallmark gene of Thaumarchaeota encodes a functional eukaryote-like topoisomerase IB [PDF]

, 2016
International audienceType IB DNA topoisomerases can eliminate torsional stresses produced during replication and transcription. These enzymes are found in all eukaryotes and a short version is present in some bacteria and viruses. Among prokaryotes, the
Collin, Sylvie, Criscuolo, Alexis, Dahmane, Narimane, Delmas, Stéphane, Desnoues, Nicole, Eberhard, Stephan, Forterre, Patrick, Gadelle, Danièle, Pommier, Yves, Sezonov, Guennadi, Zhang, Hongliang   +10 more
core   +4 more sources