Adaptive laboratory evolution and transcriptomic profiling reveal carbon-nitrogen metabolic reprogramming enabling aerobic co-fermentation of glucose and xylose in Saccharomyces cerevisiae. [PDF]
PLoS OneLópez-deÁvila LM +2 more
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Genome-scale metabolic modeling of Ruminiclostridium cellulolyticum: a microbial cell factory for valorization of lignocellulosic biomass. [PDF]
mSystemsBurgos I +4 more
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Single Mutation in <i>iolT1</i> in <i>ptsG</i>-Deficient <i>Corynebacterium glutamicum</i> Enables Growth Boost in Xylose-Containing Media. [PDF]
MicroorganismsHofer K +4 more
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Xylose Isomerase Catalysed Novel Hexose Epimerization
Biocatalysis and Biotransformation, 1999Novel hexose substrates and new epimerization reactions catalysed by the industrially important D-xylose ketol-isomerase (E.C. 5. 3. 1. 5.) are described. Isomerization equilibrium betweer D-allose and D-psicose or D-sorbose and D-gulose is obtained starting from either of the substrates.
Pastinen, O. +2 more
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Xylose isomerases from Thermotoga
2001Publisher Summary Typically present in microorganisms that grow on xylose, xylose isomerise (XI) converts xylose to xylulose, which is then phosphorylated and enters the pentose-phosphate pathway. Because it also accepts glucose as substrate, XI is used extensively to isomerize glucose to fructose in the manufacture of high fructose corn syrup (HFCS).
C, Vieille +3 more
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Structure and mechanism of D-xylose isomerase
Faraday Discussions, 1992The action of xylose isomerase depends on the presence of two divalent cations. Crystal structure analyses of the free enzyme, and of the enzyme bound to a variety of substrates and inhibitors, have provided models for a number of distinct intermediates along the reaction pathway.
D M, Blow +3 more
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Mechanism of Action of D-Xylose Isomerase
Current Protein & Peptide Science, 2000The present knowledge on the stereochemical mechanism of action of glucose (or xylose) isomerase, one of the highest tonnage industrial enzymes, is summarized. First we deal shortly with experimental methods applied to study the structure and function of this enzyme: enzyme kinetics, protein engineering, X-ray crystallography, nuclear magnetic and ...
B, Asbóth, G, Náray-Szabó
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Hyperexpression of Escherichia coli Xylose Isomerase
Biotechnology Progress, 1986AbstractThe xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl+ E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac‐xylA fusion was induced with isopropylthio β‐D‐galactopyranoside.
C A, Batt +4 more
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Overproduction of d-xylose isomerase in Escherichia coli by cloning the d-xylose isomerase gene
Enzyme and Microbial Technology, 1985Abstract The Escherichia coli d -xylose isomerase ( d -xylose ketol-isomerase, EC 5.3.1.5) gene , xyl A, has been cloned on various E. coli plasmids. However, it has been found that high levels of overproduction of the d -xylose isomerase, the protein product of the xyl A gene, cannot be accomplished by cloning the intact gene on high copy ...
Panagiotis E. Stevis, Nancy W.Y. Ho
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Immunoaffinity purification of glucose/xylose isomerase fromStreptomyces
Applied Biochemistry and Biotechnology, 1991A procedure was developed to purify glucose/xylose isomerase from cell extract of Streptomyces sp. NCIM 2730 using immunoaffinity chromatography. High-titer polyclonal antibodies were raised in rabbit using electrophoretically homogeneous glucose/xylose isomerase as an antigen.
M, Ghatge +3 more
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