Results 1 to 10 of about 27,787 (207)

Functional Elucidation of Nemopilema nomurai and Cyanea nozakii Nematocyst Venoms’ Lytic Activity Using Mass Spectrometry and Zymography

Toxins, 2017
Background: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro.
Yang Yue, Huahua Yu, Rongfeng Li
exaly   +3 more sources

An Inexpensive Staining Alternative for Gelatin Zymography Gels [PDF]

Methods and Protocols, 2019
Zymography is a widely used electrophoretic method to determine proteolytic activities in samples from various sources. The method is based on copolymerizing a suitable protein substrate within a sodium dodecyl sulfate-polyacrylamide gel.
Christian Wechselberger, Christian Doppler, David Bernhard   +2 more
doaj   +3 more sources

Determination of the fibrinogenolytic activity of Montivipera raddei (Raddeʼs mountain viper) venom [PDF]

Archives of Biological Sciences, 2022
Snake venom fibrinogenolytic enzymes have diagnostic and therapeutic value and are important for snakebite pathology. In the present study, the fibrinogenolytic activity of Montivipera raddei venom was investigated.
Atasoy Fikriye Seda, İğci Naşit
doaj   +1 more source

Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

Brazilian Archives of Biology and Technology, 2021
Ananas Comosus (also known as pineapple) is a part of Bromeliaceae family and it is consumed as food as well as folk medicine for the treatment of various diseases.
Anum Gul, Maryam Siddiqui, Habiba Arain, Sadaf Khan, Hanzala Khan, Urooj Ishrat   +5 more
doaj   +1 more source

Development of a Simple Protocol for Zymogram-Based Isolation and Characterization of Gingipains from Porphyromonas gingivalis: The Causative Agent of Periodontitis

Applied Sciences, 2023
Gingipains (RgpA, RgpB, and Kgp) are major virulence factors of the periodontitis-causing bacterium Porphyromonas gingivalis. Isolation of gingipains from the crude protein sample of P.
Eng Sze Wei, Ramasamy Kavitha, Mohammad Auwal Sa’ad, Pattabhiraman Lalitha, Neeraj Kumar Fuloria, Manickam Ravichandran, Shivkanya Fuloria   +6 more
doaj   +1 more source

The Influence of Different Bleaching Protocols on Dentinal Enzymatic Activity: An In Vitro Study

Molecules, 2022
This study aimed to investigate matrix metalloproteinase (MMP) activity in human dentin using in-situ and gelatin zymography, after at-home and in-office bleaching, related to their clinical exposure times.
Eric Mayer-Santos, Tatjana Maravic, Allegra Comba, Patricia Moreira Freitas, Giovanna Bueno Marinho, Claudia Mazzitelli, Edoardo Mancuso, Nicola Scotti, Federica Florenzano, Lorenzo Breschi, Annalisa Mazzoni   +10 more
doaj   +1 more source

Application of Zymographic and Proteomic Analysis for Identification of Intracellular and Extracellular Proteases of Vibrio cholerae Production Strains

Проблемы особо опасных инфекций, 2021
The aim of this work was to study the composition and functions of intracellular and extracellular proteases of the production Vibrio cholerae strains 569B serovar Inaba and M41 serovar Ogawa using zymographic and proteomic analysis.Materials and methods.
T. A. Polunina, N. V. Kotova, D. V. Badanin, A. V. Fedorov, O. V. Gromova, Ya. M. Krasnov, N. A. Osina   +6 more
doaj   +1 more source

Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Marine Drugs, 2020
The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive ...
Eugenia Baena, Sandra R Cunha, Tatjana Maravić, Allegra Comba, Federica Paganelli, Giulio Alessandri-Bonetti, Laura Ceballos, Franklin R Tay, Lorenzo Breschi, Annalisa Mazzoni   +9 more
doaj   +1 more source

Analysis of laccase‐like enzymes secreted by fungi isolated from a cave in northern Spain

MicrobiologyOpen, 2022
Laccases belong to a family of multicopper enzymes able to oxidize a broad spectrum of organic compounds. Despite the well‐known property of laccases to carry out bleaching and degradation of industrial dyes and polyphenolic compounds, their industrial ...
Daniel Fernández‐Remacha, Candela González‐Riancho, Miranda Lastra Osua, Aránzazu González Arce, Itxaso Montánchez, Juan María García‐Lobo, Roger Estrada‐Tejedor, Vladimir R. Kaberdin   +7 more
doaj   +1 more source

Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells. [PDF]

, 2012
Mesothelial cells are critical in the pathogenesis of post-surgical intraabdominal adhesions as well as in the deterioration of the peritoneal membrane associated with long-term peritoneal dialysis.
Djafarzadeh, Roghieh, Goyal, Pankaj, Himmelein, Susanne, Nelson, Peter J., Noessner, Elfriede, Notohamiprodjo, Susan, Ribeiro, Andrea, Sauter, Matthias, Siess, Wolfgang, Sitter, Thomas, Wörnle, Markus   +10 more
core   +13 more sources