Selective function-blocking monoclonal human antibody highlights the important role of membrane type-1 matrix metalloproteinase (MT1-MMP) in metastasis. [PDF]
, 2016 The invasion-promoting MT1-MMP is a cell surface-associated collagenase with a plethora of critical cellular functions. There is a consensus that MT1-MMP is a key protease in aberrant pericellular proteolysis in migrating cancer cells and, accordingly, a
Cieplak, Piotr +5 more
core +1 more source
Zymography: developing of the enzyme soil activity visualization method
Бюллетень Почвенного института им. В.В. Докучаева, 2023 The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms).
M. V. Timofeeva +4 more
doaj +1 more source
Co-Emergence of Specialized Endothelial Cells from Embryonic Stem Cells. [PDF]
, 2018 A well-formed and robust vasculature is critical to the health of most organ systems in the body. However, the endothelial cells (ECs) forming the vasculature can exhibit a number of distinct functional subphenotypes like arterial or venous ECs, as well ...
Douglas, Simone A +6 more
core +1 more source
Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature [PDF]
, 2008 Background: In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous proteins in Pseudoalteromonas haloplanktis TAC125.
CIRULLI C. +3 more
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Detection of Matrix Metalloproteinases by Zymography [PDF]
, 2017 Matrix metalloproteinases (MMPs) represent more than 20 zinc-containing endopeptidases that cleave internal peptide bonds, leading to protein degradation. They play a critical role in many physiological cell functions, including tissue remodeling, embryogenesis, and angiogenesis.
Rajeev B, Tajhya +2 more
openaire +2 more sources
In Drosophila Hemolymph, Serine Proteases Are the Major Gelatinases and Caseinases
InsectsAfter separation on gel zymography, Drosophila melanogaster hemolymph displays gelatinase and caseinase bands of varying sizes, ranging from over 140 to 25 kDa.
Jean-Luc Gatti +4 more
doaj +1 more source
Zymography for Picogram Detection of Lipase and Esterase Activities
Molecules, 2021 Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity ...
Andre Mong Jie Ng +2 more
doaj +1 more source
Phenotypic characterization of human prostatic stromal cells in primary cultures derived from human tissue samples [PDF]
, 2013 Emerging evidence has shown that the tumor microenvironment plays a crucial role in prostate cancer (PCa) development and progression. However, the mechanism(s) through which stromal cells regulate epithelial cells and the differences among prostatic ...
Clemente, Luigi Di +7 more
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Identification of a novel 82 kDa proMMP-9 species associated with the surface of leukaemic cells [PDF]
, 2007 MMP-9 (matrix metalloproteinase 9) plays a critical role in tumour progression. Although the biochemical properties of the secreted form of proMMP-9 are well characterized, little is known about the function and activity of cell surface-associated proMMP-
Egea, Virginia +6 more
core +1 more source
Advanced Science, EarlyView.Antibody drug conjugates deliver their cytotoxic anti‐tubulin or topoisomerase I inhibitor payloads to tumors through cancer cell receptor targeting. The released drug payloads induce cellular changes that interact with radiotherapy resulting in radiosensitization that improves cancer cell kill and stimulates anti‐tumor immune responses.
Jacqueline Lesperance +17 more
wiley +1 more source