Results 81 to 90 of about 27,806 (225)

Detection and Estimation of Active Proteinases in Biological Samples: An Optimized Protocol

Frontiers in Bioscience-Landmark
Background: Matrix metalloproteinases (MMPs) are enzymes that degrade extracellular matrix (ECM) proteins and activate cytokines and chemokines, playing a critical role in tissue remodeling.
Hebah Almarshood, Yu-Ting Zheng, Robert E. Brainard, Suresh C. Tyagi, Daniela Terson de Paleville, Michael N. Sekula, Utpal Sen   +6 more
doaj   +1 more source

Quercetin prevents progression of disease in elastase/LPS-exposed mice by negatively regulating MMP expression [PDF]

, 2010
Background Chronic obstructive pulmonary disease (COPD) is characterized by chronic bronchitis, emphysema and irreversible airflow limitation. These changes are thought to be due to oxidative stress and an imbalance of proteases and antiproteases ...
A Babusyte, A Kode, A Papi, A Sebastian, A Sydbom, AP Rogerio, AW Boots, C Kandaswami, C Tabak, CJ Vlahos, D Nowak, DC Newcomb, EH Walker, G Agullo, GA Finlay, GW Peet, HJ Park, HS Deshmukh, HS Deshmukh, I Rahman, IC Walda, IK Demedts, J Robak, JA Conquer, JC Chen, JD Hasday, JH Vernooy, JW Hwang, K Ito, K Minamoto, K Ohnishi, KF Chung, KK Meja, L Biasutto, L Larsson, L Segura-Valdez, M Comalada, M Harwood, M Ichinose, M Kasielski, M Suzuki, MK Hwang, MM Mu, N Louhelainen, O Michel, P Maestrelli, P Santus, PC Hollman, PF Mercer, PJ Jagielo, R Mahadeva, S Nanua, S Rajendrasozhan, S Shishodia, SK Biswas, SP Davies, SV Culpitt, T Shinohara, T Zheng, U Sajjan, W MacNee, Y Kaneko, Y Nakamaru, Y-T Huang   +63 more
core   +6 more sources

Guaiacol peroxidase zymography for the undergraduate laboratory [PDF]

Biochemistry and Molecular Biology Education, 2014
AbstractThis laboratory exercise presents a novel way to introduce undergraduate students to the specific detection of enzymatic activity by electrophoresis. First, students prepare a crude peroxidase extract and then analyze the homogenate via electrophoresis.
Jeff, Wilkesman, Diana, Castro, Lellys M, Contreras, Liliana, Kurz   +3 more
openaire   +2 more sources

Tumor‐Targeted FABP5/STING Cascade Promote Radiofrequency Ablation Induced Ferroptosis and Intratumoral Immune Rewiring in Hepatocellular Carcinoma

Advanced Science, Volume 12, Issue 45, December 4, 2025.
Targeted FABP5 co‐delivery nanoparticles amplify RFA‐induced ferroptosis and intratumoral immune rewiring via STING/TBK1 pathway activation in HCC. The pH‐responsive lipid nanoshell was integrated with cyclic RGD peptide (cRGD) and red blood cell membrane (RBCM), and encapsulated with superparamagnetic iron oxide (SPIO) and sgFABP5 to construct FS@RBCM/
Bufu Tang, Xiaojie Zhang, Yiting Sun, Jinhua Luo, Qiaoyou Weng, Cong Zhang, Zilin Wang, Shiji Fang, Yangrui Xiao, Liyun Zheng, Jianfei Tu, Rongfang Qiu, Yang Yang, Zhongwei Zhao, Weiqian Chen, Minjiang Chen, Jiansong Ji   +16 more
wiley   +1 more source

Emerging Biosensor Technologies for Stroke Biomarker Detection: A Comprehensive Overview

Analytical Science Advances, Volume 6, Issue 2, December 2025.
ABSTRACT Stroke remains a leading cause of morbidity and mortality worldwide, necessitating the development of rapid and reliable diagnostic tools for early detection and management. This manuscript presents an overview of innovative biosensors designed for the detection of key stroke biomarkers, including N‐terminal pro B‐type natriuretic peptide (NT ...
Ghazal Koohkansaadi, Arash Mohagheghi, Ahmad Mobed, Saeid Charsouei   +3 more
wiley   +1 more source

The Role of the Vaginal Microbiome in Preterm Premature Rupture of Membranes: A Comprehensive Review of Mechanisms and Clinical Implications

Health Science Reports, Volume 8, Issue 12, December 2025.
ABSTRACT Background and Aims Preterm premature rupture of membranes (PPROM), a complication in approximately 4.5% of pregnancies, is a leading cause of preterm birth and significant perinatal morbidity. A substantial body of evidence implicates vaginal dysbiosis a departure from a healthy, Lactobacillus‐dominant microbiome in the pathogenesis of PPROM.
Maryam Alikamali, Sakineh Mohammad‐Alizadeh‐Charandabi, Somayeh Ahmadi, Mohammad Yousef Memar, Mahnaz Shahnazi   +4 more
wiley   +1 more source

An improved collagen zymography approach for evaluating the collagenases MMP-1, MMP-8, and MMP-13

BioTechniques, 2017
Collagen zymography is an SDS-PAGE–based method for detecting both the proenzyme and active forms of collagenases. Although collagen zymography is used for assessment of the matrix metalloproteinases MMP-1 and MMP-13, it can be difficult to detect these ...
Seniz Inanc, Didem Keles, Gulgun Oktay
doaj   +1 more source

Matrix Metalloproteinases and Glaucoma Treatment. [PDF]

, 2020
Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that degrade extracellular matrix (ECM) components such as collagen and have important roles in multiple biological processes, including development and tissue remodeling, both in ...
Dibas, Mohammed, Robinson, Michael R, Stamer, W Daniel, Weinreb, Robert N   +3 more
core  

HDAC 1 and 6 modulate cell invasion and migration in clear cell renal cell carcinoma [PDF]

, 2016
Indexación: Web of ScienceBackground: Class I histone deacetylases (HDACs) have been reported to be overexpressed in clear cell renal cell carcinoma (ccRCC), whereas the expression of class II HDACs is unknown.
Attwood, Kris, Chintala, Sreenivasulu, Ciamporcero, Eric, Conroy, Dylan, Das, Gokul, Ellis, Leigh, Huang, Ray, Ku, ShengYu, Miles, Kiersten Marie, Orillion, Ashley, Pili, Roberto, Ramakrishnan, Swathi, Shen, Li, Sotomayor, Paula, Swetzig, Wendy   +14 more
core   +2 more sources

Dissecting Shared Genetic Architecture of Thoracic Aortic Aneurysm and Aortic Related Traits and Identifying SplA/Ryanodine Receptor Domain and SOCS Box Containing 1 Involved in Smooth Muscle Phenotype Switching and Cell Senescence Through Alternative Splicing

The FASEB Journal, Volume 39, Issue 22, 30 November 2025.
The flowchart of the study. BAPN, β‐aminopropionitrile; FUMA, Functional Mapping and Annotation; GO, Gene Ontology; GWAS, genome‐wide association study; KEGG, Kyoto encyclopedia of genes and genomes; LDSC, LD score regression; MR‐JTI, mendelian randomization joint‐tissue imputation; MTAG, multi‐trait analysis of GWAS; siRNA, small interfering RNA ...
Qingyang Song, Tongxin Chu, Quan Liu, Rennan Weng, Huayang Li, Mengya Liang, Zhongkai Wu   +6 more
wiley   +1 more source