Advanced Science, EarlyView.Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green +15 more
wiley +1 more source
SYSTEMIC KANAMYCIN TREATMENT HAS NO EFFECT ON CELL NUMBERS IN THE MOUSE UTRICULAR MACULA
Image Analysis and Stereology, 2011 The aim of the present study is to estimate the total number of the sensory hair cells (chalice innervated and bouton innervated) and supporting cells in the mouse utricular sensory epithelium at two different time points after systemic kanamycin ...
Mette Kirkegaard +3 more
doaj +1 more source
Alarmone Ap4A is elevated by aminoglycoside antibiotics and enhances their bactericidal activity
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance This paper demonstrates that aminoglycoside antibiotics induce the production of the Ap4A in bacteria. Increased intracellular Ap4A, in turn, promotes bacterial cell killing by this class of antibiotics, which correlated well with elevated ...
X. Ji +8 more
semanticscholar +1 more source
Does reduced usage of antibiotics in livestock production mitigate the spread of antibiotic resistance in soil, earthworm guts, and the phyllosphere? [PDF]
, 2020 The overuse of antibiotics in animal husbandry is widespread and believed to significantly contribute to the selection of antibiotic resistance genes (ARGs) in animals.
Daniell, Tim +5 more
core +3 more sources
Restoring Iron Homeostasis via Smoothened Inhibition: A Novel Strategy Against Hearing Loss
Advanced Science, EarlyView.. ABSTRACT Sensorineural hearing loss (SNHL) induced by noise or aminoglycoside antibiotics is a significant public health concern without any FDA‐approved pharmaceutical therapies. Dysregulation of iron homeostasis and its subsequently induced ferroptosis has increasingly been identified as a key mechanism underlying cochlear hair cell (HC) damage ...
Huanyu Mao +9 more
wiley +1 more source
Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus
Advanced Science, EarlyView.This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan +11 more
wiley +1 more source
Aminoglycoside interactions and impacts on the eukaryotic ribosome
Proceedings of the National Academy of Sciences of the United States of America, 2017 Significance Aminoglycosides are well known as antibiotics that target the bacterial ribosome. However, they also impact the eukaryotic translation mechanism to promote read-through of premature termination codons (PTCs) in mRNA.
I. Prokhorova +9 more
semanticscholar +1 more source
FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel [PDF]
, 2001 Hair cells in mouse cochlear cultures are selectively labeled by brief exposure to FM1-43, a styryl dye used to study endocytosis and exocytosis. Real-time confocal microscopy indicates that dye entry is rapid and via the apical surface.
Gale, J.E. +4 more
core +1 more source
Time-programmable drug dosing allows the manipulation, suppression and reversal of antibiotic drug resistance in vitro [PDF]
, 2017 Multi-drug strategies have been attempted to prolong the efficacy of existing antibiotics, but with limited success. Here we show that the evolution of multi-drug-resistant Escherichia coli can be manipulated in vitro by administering pairs of ...
Cronin, Leroy +5 more
core +1 more source
The small quinolone derived compound HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo. [PDF]
, 2020 HT61 is a small quinolone-derived compound previously demonstrated to exhibit bactericidal activity against gram-positive bacteria including methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). When
Amison, RT +6 more
core +1 more source