Results 191 to 200 of about 7,439 (222)

Artificial ribonucleases. 5. Synthesis and ribonuclease activity of tripeptides composed of amino acids involved in catalytic centers of natural ribonucleases

Russian Chemical Bulletin, 2004
The characteristic features of the spatial arrangement of the main functional groups involved in catalytic centers of ribonucleases and nucleases were revealed by computer analysis of the catalytic centers of these enzymes. Based on the results of computer simulation, tripeptides containing Lys, Arg, His or Hia, Thr, and Asn in different combinations ...
I. L. Kuznetsova, N. S. Zhdan, M. A. Zenkova, V. V. Vlassov, V. N. Sil\\"nikov   +4 more
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Identification of efficient and sequence specific bimolecular artificial ribonucleases by a combinatorial approach

Chem. Commun., 2008
Chemically modified nucleotide monomers were incorporated into adjacent terminal positions of two separate oligonucleotides complementary to an RNA target; all possible combinations of the catalytic units were tested, resulting in an artificial nuclease that showed high activity and catalytic turnover.
Kalek, Marcin, Benediktson, Peter, Vester, Birte, Wengel, Jesper   +3 more
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A mechanism of the toxicity of artificial ribonucleases for human cancer cells

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, 2010
The ability of artificial ribonucleases, low molecular weight compounds exhibiting RNA cleavage in vitro, to cause human cancer cell death in a concentration-dependent manner has been studied. The cytotoxic effect of artificial ribonucleases on cells appeared at rather low concentrations of these compounds (10−5 M).
E. B. Logashenko, I. L. Kuznetsova, E. I. Ryabchikova, V. V. Vlassov, M. A. Zenkova   +4 more
openaire   +1 more source

Artificial ribonucleases: From combinatorial libraries to efficient catalysts of RNA cleavage

Bioorganic Chemistry, 2006
Combinatorial libraries of small organic compounds capable of cleaving RNA were synthesized. The compounds contain benzene ring substituted with two residues of bis quaternary salt of diazabicyclo[2.2.2]octane (DABCO) bearing hydrophobic fragments of different length and structure, attached to DABCO at the bridge position.
N, Kovalev, E, Burakova, V, Silnikov, M, Zenkova, V, Vlassov   +4 more
openaire   +2 more sources

Artificial ribonucleases.

Advances in inorganic biochemistry, 1994
Many inorganic and organic compounds promote the reactions catalyzed by RNase A. Both the transesterification step, where a 2',3'-cyclic phosphate is formed with concomitant cleavage of RNA, and the hydrolysis step, where the 2',3'-cyclic phosphate is converted to a phosphate monoester, may be mimicked with compounds that are readily synthesized in the
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Influenza virus inactivated by artificial ribonucleases as a prospective killed virus vaccine

Vaccine, 2012
The inactivation of viral particles with agents causing minimal damage to the structure of surface epitopes is a well-established approach for the production of killed virus vaccines. Here, we describe new agents for the inactivation of influenza virus, artificial ribonucleases (aRNases), which are chemical compounds capable of cleaving RNA molecules ...
Antonina A, Fedorova, Elena P, Goncharova, Mikhail P, Kovpak, Valentin V, Vlassov, Marina A, Zenkova   +4 more
openaire   +2 more sources

Molecular design of artificial hydrolytic nucleases and ribonucleases.

Nucleic acids symposium series, 1993
Artificial nucleases and ribonucleases, which selectively hydrolyze the specific phosphodiester linkages in DNAs and RNAs, are prepared by the attachment of organic and inorganic catalysts to DNA oligomers as sequence-recognizing moieties. A hybrid of ethylenediamine and a 19-mer DNA selectively hydrolyzes tRNA(Phe) at the 3'-side of C63.
M, Komiyama, T, Inokawa, T, Shiiba, N, Takeda, K, Yoshinari, M, Yashiro   +5 more
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Artificially induced microheterogeneity in ribonuclease

Biochemical and Biophysical Research Communications, 1960
R, SHAPIRA, S, PARKER
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Site-Specific Artificial Ribonucleases: Conjugates of Oligonucleotides with Catalytic Groups

2004
Design of site-specific artificial ribonucleases is one of the most challenging tasks in RNA targeting (Morrow 1994; Haner et aL 1998; Oivanen et aL 1998; Sil’nikov and Vlassov 2001). Apart from being useful molecular biology tools (Huber 1993; Giege et aL 2000), such compounds may provide new opportunities for design of therapeutics targeting specific
M. A. Zenkova, N. G. Beloglazova
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Sequence-Selective Artificial Ribonucleases

2001
M, Komiyama, J, Sumaoka, A, Kuzuya, Y, Yamamoto   +3 more
openaire   +2 more sources