Results 1 to 10 of about 7,909 (199)

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model [PDF]

BMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff   +6 more
doaj   +4 more sources

Identification of endogenous reference genes for RT-qPCR analysis in breast cancer and matched adjacent tissues [PDF]

Front Oncol
BackgroundReal-time quantitative PCR (RT-qPCR), essential for gene expression and biomarker studies, requires stable endogenous reference genes (RGs) for normalization. This study aimed to identify consistently expressed RGs in breast cancer and adjacent
Meng Y, Wang Y, Xu Z, Qiao Z, Liu C, Chen Y, Xu Y, Zhang K.   +7 more
europepmc   +2 more sources

Identification of stable internal reference genes for expression analysis in the liver and pancreas of diabetic mouse (Mus musculus L.) models under physiological, pathological and treatment conditions. [PDF]

PLoS One
Accurate gene expression analysis via reverse transcription quantitative real-time PCR is vital for studying diabetes mellitus. Accurate and reliable normalization of RT-qPCR data, in accordance with the MIQE guidelines, demands a group of at least two ...
Nong VD, Nguyen DQ, Do TT, Giang Tran TH, Nguyen TH, Le TT, Tran HT, Nguyen HH.   +7 more
europepmc   +2 more sources

Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations. [PDF]

BMC Microbiol
Pseudomonas aeruginosa can efficiently degrades petroleum hydrocarbons, but its degradation rate is affected by hydrocarbon pollutant concentrations, which must be neither too high nor too low. To quantitatively analyze P.
Wang H, Wang X, Chen J, Zhang Y, Liu J, Xia F, Li J, Wu T, Liu L.   +8 more
europepmc   +2 more sources

Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections [PDF]

Virology Journal, 2005
Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow ...
Müller Marcel A, Bae Hi-Gung, Thulke Stefanie, Radonić Aleksandar, Siegert Wolfgang, Nitsche Andreas   +5 more
doaj   +2 more sources

Validation of Housekeeping Genes in the Brains of Rats Submitted to Chronic Intermittent Hypoxia, a Sleep Apnea Model [PDF]

, 2014
Obstructive sleep apnea (OSA) is a syndrome characterized by intermittent nocturnal hypoxia, sleep fragmentation, hypercapnia and respiratory effort, and it has been associated with several complications, such as diabetes, hypertension and obesity ...
Chagas, Jair Ribeiro, Julian, Guilherme Silva, Oliveira, Renato Watanabe de, Perry, Juliana Cini, Tufik, Sergio   +4 more
core   +24 more sources

Characterization of reference genes in rare minnow, Gobiocypris rarus (Actinopterygii: Cypriniformes: Cyprinidae), in early postembryonic development and in response to EDCs treatment [PDF]

Acta Ichthyologica et Piscatoria, 2013
Background. Endocrine disrupting chemicals (EDCs) are natural and anthropogenic compounds discharged into the environment known to disrupt the endocrine system of humans and animals by mimicking functions of steroids in vivo.
F. Qin, L. Wang, S. Liu, Z. Wang
doaj   +3 more sources

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

Scientific Reports, 2021
Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque, Fernando C. A. Fonseca, Leonardo S. Boiteux, Rafaela C. F. Borges, Robert N. G. Miller, Carlos A. Lopes, Elineide B. Souza, Maria Esther N. Fonseca   +7 more
doaj   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

Biologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress

Biologia Plantarum, 2020
Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj   +1 more source