Results 11 to 20 of about 6,321 (156)

Reference Gene Validation for RT–qPCR in PBMCs from Asthmatic Patients with or without Obesity

Methods and Protocols, 2022
Obesity is known to impair the efficacy of glucocorticoid medications for asthma control. Glucocorticoid-induced gene expression studies may be useful to discriminate those obese asthmatic patients who present a poor response to glucocorticoids.
Marina Bantulà, Ebymar Arismendi, César Picado, Joaquim Mullol, Jordi Roca-Ferrer, Valeria Tubita   +5 more
doaj   +1 more source

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]

PLoS ONE, 2015
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang, Hui Li, Huipeng Pan, Yabin Ma, Deyong Zhang, Yong Liu, Zhanhong Zhang, Changying Zheng, Dong Chu   +8 more
doaj   +1 more source

Housekeeping gene stability in Pesudomonas aeruginosa PAO1 under the pressure of commonly used antibiotics in molecular microbiology assays

Frontiers in Microbiology, 2023
Pseudomonas aeruginosa is an opportunistic human pathogen notorious for its remarkable capacity of multi-drug resistance, and has become one of the most important model bacteria in clinical bacteriology research.
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu   +8 more
doaj   +1 more source

Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Animals, 2020
Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood.
Linjie Wang, Xingyue Chen, Tianzeng Song, Xujia Zhang, Siyuan Zhan, Jiaxue Cao, Tao Zhong, Jiazhong Guo, Li Li, Hongping Zhang, Yan Wang   +10 more
doaj   +1 more source

Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]

, 2016
This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John, Bowman, Alan S, Campbell, Ewan M, McIntosh, Catriona H, Woods, Debra J., Zinser, Erich   +5 more
core   +1 more source

Validation of reference genes for the normalization of the RT-qPCR in peripheral blood mononuclear cells of patients with Takayasu arteritis [PDF]

Jichu yixue yu linchuang, 2021
Objective To validate proper reference genes for quantitative real-time polymerase chain reaction (RT-qPCR) used for comparing mRNA expression levels in Takayasu arteritis' (TAK) and healthy controls' (HC) peripheral blood mononuclear cells (PBMC ...
TIAN Yi-xiao, LI Jing
doaj  

Feeding Stimulates Sphingosine-1-Phosphate Mobilization in Mouse Hypothalamus. [PDF]

, 2019
Previous studies have shown that the sphingolipid-derived mediator sphingosine-1-phosphate (S1P) reduces food intake by activating G protein-coupled S1P receptor-1 (S1PR1) in the hypothalamus.
Misto, Alessandra, Piomelli, Daniele, Realini, Natalia, Vozella, Valentina   +3 more
core   +1 more source

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

Bio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen, Joanna Lopacinska-Jørgensen, Claus Høgdall, Estrid Høgdall   +3 more
doaj   +1 more source

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]

, 2008
Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas, A Radonic, A Szabo, A Zapata, AL Filby, B Liss, C Ramakers, CL Andersen, EO Koppang, F Haller, G Rauch, HC Ingerslev, J Vandesompele, Joern P Scharsack, JP Scharsack, K Pechhold, KM Wegner, MF Oleksiak, MW Pfaffl, N Nicot, PA Olsvik, S Becker, SA Bustin, SA Bustin, Sascha Hibbeler, SF Gonzalez, SM Jorgensen, Sven Becker, TBH Reusch, V Ransbotyn   +29 more
core   +3 more sources

microRNAs for qPCR Normalization Under Morphofunctional Conditions in Bovine Sperm (Bos taurus). [PDF]

Mol Reprod Dev
Identification of the most stable microRNAs in Bos taurus semen for qPCR analysis under sperm motility and morphology conditions. ABSTRACT Cattle represents one of the most common and widely distributed categories of large ruminants, with well‐established production practices.
de Souza LP, Nunes LS, Salvi LC, Gonçalves LDS, Reis LFVD, Acosta IB, Corcini CD, Junior ASV, Barreto FG, Vieira MB, Silveira DC, Vieira JM, Ilha GF, Domingues WB, Campos VF.   +14 more
europepmc   +2 more sources