Results 21 to 30 of about 7,909 (199)

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

Bio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen, Joanna Lopacinska-Jørgensen, Claus Høgdall, Estrid Høgdall   +3 more
doaj   +1 more source

Feeding Stimulates Sphingosine-1-Phosphate Mobilization in Mouse Hypothalamus. [PDF]

, 2019
Previous studies have shown that the sphingolipid-derived mediator sphingosine-1-phosphate (S1P) reduces food intake by activating G protein-coupled S1P receptor-1 (S1PR1) in the hypothalamus.
Misto, Alessandra, Piomelli, Daniele, Realini, Natalia, Vozella, Valentina   +3 more
core   +1 more source

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]

, 2008
Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas, A Radonic, A Szabo, A Zapata, AL Filby, B Liss, C Ramakers, CL Andersen, EO Koppang, F Haller, G Rauch, HC Ingerslev, J Vandesompele, Joern P Scharsack, JP Scharsack, K Pechhold, KM Wegner, MF Oleksiak, MW Pfaffl, N Nicot, PA Olsvik, S Becker, SA Bustin, SA Bustin, Sascha Hibbeler, SF Gonzalez, SM Jorgensen, Sven Becker, TBH Reusch, V Ransbotyn   +29 more
core   +3 more sources

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells [PDF]

, 2012
Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection.
Baquero Perez, Belinda, Brookes, Sharon M., Brown, Ian H., Chang, Kin-Chow, Dunham, Stephen P., Kuchipudi, Suresh V., Nelli, Rahul K., Sebastian, Sujith, Slomka, Marek J., Tellabati, Meenu, White, Gavin A.   +10 more
core   +7 more sources

Selection and validation of appropriate reference genes for RT–qPCR analysis of Nitraria sibirica under various abiotic stresses

BMC Plant Biology, 2022
Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu, Xiuyan Yang, Jianfeng Zhu, Xiuping Wang, Jiaxin Liu, Jiping Wang, Haiwen Wu, Huilong Zhang, Huaxin Zhang   +8 more
doaj   +1 more source

Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset [PDF]

, 2014
Background Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results.
Ana Viñas, Belén G Pardo, Diego Robledo, Jorge Hernández-Urcera, Laura Sánchez, Paulino Martínez, Rosa M Cal   +6 more
core   +4 more sources

Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]

PLoS ONE, 2014
Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu, Nian Xin, Yi Zhai, Liming Jiang, Jieming Zhai, Quanqi Zhang, Jie Qi   +6 more
doaj   +1 more source

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Megalurothrips usitatus (thysanoptera: thripidae)

Frontiers in Physiology, 2023
Introduction: Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects.
Qingfang Hou, Linlin Yuan, Haifeng Jin, Han Yan, Fen Li, Shao-ying Wu   +5 more
semanticscholar   +1 more source

Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)

BMC Research Notes, 2019
Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva, Marcos Cesar Gonçalves, Michael dos Santos Brito, Paula Macedo Nóbile, Larissa Mara de Andrade, Cibele Nataliane Medeiros, Silvana Creste, Luciana Rossini Pinto   +7 more
doaj   +1 more source

Screening and Verification of Reference Genes for Analysis of Gene Expression in Garlic (Allium sativum L.) under Cold and Drought Stress

Plants, 2023
The principal objective of this study was to screen and verify reference genes appropriate for gene expression evaluation during plant growth and development under distinct growth conditions.
Qizhang Wang, Chunqian Guo, Shipeng Yang, Q. Zhong, Jie Tian   +4 more
semanticscholar   +1 more source