18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells [PDF]
, 2012 Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection.
Baquero Perez, Belinda +10 more
core +7 more sources
BMC Plant Biology, 2022 Background Nitraria sibirica Pall. is a halophytic shrub with strong environmental adaptability that can survive in extremely saline-alkali and drought-impacted environments. Gene expression analysis aids in the exploration of the molecular mechanisms of
Aishuang Hu +8 more
doaj +1 more source
Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]
PLoS ONE, 2014 Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu +6 more
doaj +1 more source
Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey () tissues [PDF]
, 2008 Background: The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of ...
K Ahn +8 more
core +1 more source
Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset [PDF]
, 2014 Background Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results.
Ana Viñas +6 more
core +4 more sources
BMC Research Notes, 2019 Objective The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome ...
Marcel Fernando da Silva +7 more
doaj +1 more source
Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc [PDF]
, 2009 Background: Quantitative real-time polymerase chain reaction (RT-qPCR) is valuable for studying the molecular events underlying physiological and behavioral phenomena.
Maria Sirakov +6 more
core +1 more source
Animal, 2016 Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition.
X. Li +6 more
doaj +1 more source
Harbin: a quantitation PCR analysis tool [PDF]
, 2016 Objectives: To enable analysis and comparisons of different relative quantitation experiments, a web-browser application called Harbin was created that uses a quantile-based scoring system for the comparison of samples at different time points and ...
Aldrich, Dirk J. +3 more
core +1 more source
Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses. [PDF]
PLoS ONE, 2013 Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions.
Jian Sang +5 more
doaj +1 more source