Results 31 to 40 of about 7,909 (199)

Validation of reference genes for gene expression analysis in olive () mesocarp tissue by quantitative real-time RT-PCR [PDF]

, 2014
BACKGROUND: Gene expression analysis using quantitative reverse transcription PCR (qRT-PCR) is a robust method wherein the expression levels of target genes are normalised using internal control genes, known as reference genes, to derive changes in gene ...
Johnson, Joshua, Ray, Debashree
core   +8 more sources

Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells

Animal, 2016
Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition.
X. Li, K. Huang, F. Chen, W. Li, S. Sun, X.-E. Shi, G. Yang   +6 more
doaj   +1 more source

Identification of stably expressed Internal Control Genes (ICGs) for normalization of expression data in liver of C57BL/6 mice injected with beta casomorphins.

PLoS ONE, 2023
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health.
Anurag Kumar, Monika Sodhi, Manishi Mukesh, Amandeep Kaur, Gaurav Bhakri, Vipul Chaudhary, Preeti Swami, Vishal Sharma, Ashok Kumar Mohanty, Ranjit S Kataria   +9 more
doaj   +1 more source

Validation of control genes and a standardised protocol for quantifying gene expression in the livers of C57BL/6 and ApoE−/− mice [PDF]

, 2018
The liver plays a critical role in food and drug metabolism and detoxification and accordingly influences systemic body homeostasis in health and disease.
AN Heinloth, BJ Ripley, C Matziouridou, CL Andersen, CY Zhong, D Wang, DE Malarkey, H Jacobsson, J Vandesompele, L Bavia, L Heyman-Linden, L Li, LA Cox, M Feng, MA Aller, MW Pfaffl, NN Ngalame, PNA Martins, R Martinez-Beamonte, R Schierwagen, RM Heidker, S Gangadaran, T Kessoku, Y Keigo, ZR Xu   +24 more
core   +1 more source

I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in Arabidopsis thaliana

Biomolecules, 2023
Quantitative real-time polymerase chain reaction (qPCR) is a widely used method to analyse the gene expression pattern in the reproductive tissues along with detecting gene levels in mutant backgrounds.
Maria João Ferreira, Jessy Silva, Sara C Pinto, S. Coimbra   +3 more
semanticscholar   +1 more source

Harbin: a quantitation PCR analysis tool [PDF]

, 2016
Objectives: To enable analysis and comparisons of different relative quantitation experiments, a web-browser application called Harbin was created that uses a quantile-based scoring system for the comparison of samples at different time points and ...
Aldrich, Dirk J., Bester, Rachelle, Maree, Hans J., Pepler, Pieter T.   +3 more
core   +1 more source

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Methods and Protocols, 2023
Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ between tissue ...
Andrea H. Dragon, Cassie J. Rowe, Alisha Rhodes, Olivia L. Pak, Thomas A. Davis, E. Ronzier   +5 more
semanticscholar   +1 more source

Selection and validation of reference genes for real-time quantitative PCR in hyperaccumulating ecotype of Sedum alfredii under different heavy metals stresses. [PDF]

PLoS ONE, 2013
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well as under different experimental conditions.
Jian Sang, Xiaojiao Han, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo   +5 more
doaj   +1 more source

Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data [PDF]

, 2007
Background Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires the transformation of data through a
Clements, Dylan N., Day, Philip, Day, Philip J R, Maccoux, Lindsey J., Marriage, Fiona, Salway, Fiona   +5 more
core   +4 more sources

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source