Results 31 to 40 of about 6,321 (156)

Selection of reference genes for quantitative RT-PCR studies in striped dolphin () skin biopsies [PDF]

, 2006
Background: Odontocete cetaceans occupy the top position of the marine food-web and are particularly sensitive to the bioaccumulation of lipophilic contaminants.
SPINSANTI, G., PANTI, C., LAZZERI, E., MARSILI, L., CASINI, S., FRATI, F., FOSSI, M. C.   +6 more
core   +1 more source

Signalling with retinoids in the human lung: validation of new tools for the expression study of retinoid receptors. [PDF]

, 2009
International audienceBACKGROUND: Retinoid Receptors are involved in development and cell homeostasis. Alterations of their expressions have been observed in lung cancer.
Poulain, Stéphane, Lacomme, Stéphanie, Battaglia-Hsu, Shyue-Fang, Du Manoir, Stanislas, Brochin, Lydia, Vignaud, Jean-Michel, Martinet, Nadine   +6 more
core   +3 more sources

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source

Identification of stably expressed Internal Control Genes (ICGs) for normalization of expression data in liver of C57BL/6 mice injected with beta casomorphins.

PLoS ONE, 2023
In recent years, beta-casomorphin peptides (BCM7/BCM9) derived from the digestion of cow milk have drawn a lot of attention world over because of their proposed impact on human health.
Anurag Kumar, Monika Sodhi, Manishi Mukesh, Amandeep Kaur, Gaurav Bhakri, Vipul Chaudhary, Preeti Swami, Vishal Sharma, Ashok Kumar Mohanty, Ranjit S Kataria   +9 more
doaj   +1 more source

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions. [PDF]

PLoS ONE, 2015
Reverse Transcription quantitative PCR (RT-qPCR) is one of the most important techniques for gene expression profiling due to its high sensibility and reproducibility.
Tatiane Timm Storch, Camila Pegoraro, Taciane Finatto, Vera Quecini, Cesar Valmor Rombaldi, César Luis Girardi   +5 more
doaj   +1 more source

Reference gene validation for qPCR in rat carotid body during postnatal development [PDF]

, 2011
Background The carotid bodies are the main arterial oxygen chemoreceptors in mammals. Afferent neural output from the carotid bodies to brainstem respiratory and cardiovascular nuclei provides tonic input and mediates important protective responses to ...
Insook Kim, Dongjin Yang, Xinyu Tang, John L Carroll   +3 more
core   +2 more sources

Abundance, efficiency, and stability of reference transcript expression in a seasonal rodent: The Siberian hamster

PLoS ONE, 2022
Quantitative PCR (qPCR) is a common molecular tool to analyse the expression of transcripts in non-traditional animal models. Most animals experience tissue-specific seasonal changes in cell structure, growth, and cellular function. As a consequence, the
Calum Stewart, Timothy A. Liddle, Tyler J. Stevenson   +2 more
doaj   +2 more sources

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source