Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi
Scientific Reports, 2023 Naegleria gruberi is a free-living amoeboflagellate commonly found in freshwater and in soils around the world. It is a non-pathogenic relative of Naegleria fowleri, which is the etiologic agent of Primary Amoebic Meningoencephalitis (PAM).
Tania Martín-Pérez +2 more
doaj +1 more source
Validation of control genes and a standardised protocol for quantifying gene expression in the livers of C57BL/6 and ApoE−/− mice [PDF]
, 2018 The liver plays a critical role in food and drug metabolism and detoxification and accordingly influences systemic body homeostasis in health and disease.
AN Heinloth +24 more
core +1 more source
Validation of reference genes for gene expression analysis in olive () mesocarp tissue by quantitative real-time RT-PCR [PDF]
, 2014 BACKGROUND: Gene expression analysis using quantitative reverse transcription PCR (qRT-PCR) is a robust method wherein the expression levels of target genes are normalised using internal control genes, known as reference genes, to derive changes in gene ...
Johnson, Joshua, Ray, Debashree
core +8 more sources
Guangdong nongye kexue【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU +6 more
doaj +1 more source
Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]
Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus +50 more
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Identification and evaluation of suitable reference genes for gene expression studies in the whitefly Bemisia tabaci (Asia I) by reverse transcription quantitative realtime PCR [PDF]
, 2014 This study presents a reliable method for performing reverse transcription quantitative realtime PCR (RT-qPCR) to measure gene expression in the whitefly Bemisia tabaci (Asia I) (Gennadius) (Hemiptera: Aleyrodidae), utilising suitable reference genes for
Carl Collins +6 more
core +1 more source
Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)
, 2019 RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim +3 more
core +1 more source
Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue [PDF]
, 2018 Lymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the ...
Cho-Isoda Yukiko +17 more
core +2 more sources
Cytomolecular and In Vitro Toxicity Studies on Bisphenol A Effect on Ovine Granulosa Cells
Veterinary Medicine International, Volume 2026, Issue 1, 2026.Bisphenol A (BPA), an organic environment chemical, has extensive presence in consumer goods and food/feed items. The present study aimed to explore how BPA influenced the viability, proliferation, apoptosis, and steroidogenic activity of ovine ovarian granulosa cells (GCs).
Poonam Kumari Singh +5 more
wiley +1 more source
Construction of an adult barnacle () cDNA library and selection of reference genes for quantitative RT-PCR studies [PDF]
, 2009 De Gregoris TB, Borra M, Biffali E, et al. Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies. BMC Molecular Biology.
Tristano Bacchetti De Gregoris +7 more
core +2 more sources