Results 41 to 50 of about 7,909 (199)

Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue [PDF]

, 2018
Lymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the ...
Cho-Isoda Yukiko, Hori Mitsuo, Iijima Tatsuo, Inada Katsushige, Ishiguro Shingo, Kojima Hiroshi, Oki Akinori, Okoshi Yasushi, Saito Hitoaki, Shimazui Toru, Suzuki Hisashi, Tamaki Yoshio, 大越 靖, 小島 寛, 島居 徹, 沖 明典, 玉木 義雄, 鈴木 久史   +17 more
core   +2 more sources

Comparative Analysis of the Expression Profiles of Pathogenesis-Related Genes in Tomato Systemically Infected with Tobacco Mosaic and Cucumber Mosaic Viruses

International Journal of Plant Biology, 2023
In this study, we used RT-qPCR to examine how PR genes were expressed in model tomato (Solanum lycopersicum L.) plants that had been infected with TMV or CMV. Under greenhouse conditions, the indirect ELISA data showed that both viruses were detected for
Dalia G. Aseel, Sherien E. Sobhy, M. Samy, E. Hamdy, S. Behiry, A. Abdelkhalek   +5 more
semanticscholar   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions. [PDF]

PLoS ONE, 2015
Reverse Transcription quantitative PCR (RT-qPCR) is one of the most important techniques for gene expression profiling due to its high sensibility and reproducibility.
Tatiane Timm Storch, Camila Pegoraro, Taciane Finatto, Vera Quecini, Cesar Valmor Rombaldi, César Luis Girardi   +5 more
doaj   +1 more source

Identification and evaluation of suitable reference genes for gene expression studies in the whitefly Bemisia tabaci (Asia I) by reverse transcription quantitative realtime PCR [PDF]

, 2014
This study presents a reliable method for performing reverse transcription quantitative realtime PCR (RT-qPCR) to measure gene expression in the whitefly Bemisia tabaci (Asia I) (Gennadius) (Hemiptera: Aleyrodidae), utilising suitable reference genes for
Carl Collins, David Bailey, Dinsdale A, John Colvin, Mitulkumar V. Patel, Scharlaken B, Susan Seal   +6 more
core   +1 more source

Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

Scientific Reports, 2021
Choroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for
Kim Hoa Ho, Annarita Patrizi
semanticscholar   +1 more source

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

Insects, 2022
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is a momentous tool for calculating the expression levels of targeted genes across various experimental conditions.
Chen-Hui Shen, Liyun Peng, Yu-Xing Zhang, Hualiang Zeng, Hong Yu, Lin Jin, Guo-Qing Li   +6 more
semanticscholar   +1 more source

Abundance, efficiency, and stability of reference transcript expression in a seasonal rodent: The Siberian hamster

PLoS ONE, 2022
Quantitative PCR (qPCR) is a common molecular tool to analyse the expression of transcripts in non-traditional animal models. Most animals experience tissue-specific seasonal changes in cell structure, growth, and cellular function. As a consequence, the
Calum Stewart, Timothy A. Liddle, Tyler J. Stevenson   +2 more
doaj   +2 more sources

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source