Results 11 to 20 of about 4,252 (213)

Identification of Chiral-Specific Carbon Nanotube Binding Peptides Using a Modified Biopanning Method

Chemosensors, 2021
Peptides can recognize and selectively bind to a wide variety of materials dependent on both their surface properties and the environment. Biopanning with phage or cell peptide display libraries can identify material-specific binding peptides.
Steve S Kim, Rajesh R Naik
exaly   +4 more sources

Generation of Nano‐Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular Vesicles [PDF]

Journal of Extracellular Biology
Handling membrane proteins (MPs) in their native environment is crucial for ensuring proper folding and stability. Extracellular vesicles (EVs) offer a superior alternative to detergents and artificial lipid environments.
Alexis Dogliani, Laura Fradale, Céline Valeille, Christian Cambillau, Emmanuelle Jacquin‐Joly, Alain Roussel, Josiane Kassis‐Sahyoun   +6 more
doaj   +3 more sources

The Use of Heterologous Antigens for Biopanning Enables the Selection of Broadly Neutralizing Nanobodies Against SARS-CoV-2 [PDF]

Antibodies
Background: Since the emergence of SARS-CoV-2 in the human population, the virus genome has undergone numerous mutations, enabling it to enhance transmissibility and evade acquired immunity.
Vazirbek S. Aripov, Anna V. Zaykovskaya, Ludmila V. Mechetina, Alexander M. Najakshin, Alexander A. Bondar, Sergey G. Arkhipov, Egor A. Mustaev, Margarita G. Ilyina, Sophia S. Borisevich, Alexander A. Ilyichev, Valentina S. Nesmeyanova, Anastasia A. Isaeva, Ekaterina A. Volosnikova, Dmitry N. Shcherbakov, Natalia V. Volkova   +14 more
doaj   +3 more sources

Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets [PDF]

Communications Biology, 2023
In vitro biopanning platforms using synthetic phage display antibody libraries have enabled the identification of antibodies against antigens that were once thought to be beyond the scope of immunization.
Dong hee Chung, Sophie Kong, Nicholas J. Young, Shih-Wei Chuo, Jamie V. Shiah, Emily J. Connelly, Peter J. Rohweder, Alexandra Born, Aashish Manglik, Jennifer R. Grandis, Daniel E. Johnson, Charles S. Craik   +11 more
doaj   +2 more sources

Mimotopes selected by biopanning with high-titer HIV-neutralizing antibodies in plasma from Chinese slow progressors

Brazilian Journal of Infectious Diseases, 2012
OBJECTIVE: One approach to identifying HIV-1 vaccine candidates is to dissect the natural antiviral immune response in treatment-naïve individuals infected for over ten years, considered slow progressor patients (SPs).
Xiaoli Zhang, Xiaoxu Han, Di Dai, Bao Mingjia, Zining Zhang, Min Zhang, Tristan Bice, Min Zhao, Yaming Cao, Hong Shang   +9 more
doaj   +5 more sources

Peptide Receptor-Functionalized AuNP-Embedded Zwitterionic Biopolymeric Nanohydrogel for Electrochemical Sclerostin Sensing. [PDF]

Adv Sci (Weinh)
We report an electrochemical biosensor that employs biopanning‐derived affinity peptides as selective receptors on a multifunctional chitosan–zwitterionic–gold nanoparticle (CS–ZI–AuNPs) nanohydrogel interface. The CS–ZI–AuNPs nanohydrogel exhibits antifouling properties, abundant sites for stable peptide immobilization, and efficient electrochemical ...
Yang HJ, Shin JH, Jo M, Lee C, Yi H, Ali ABH, Park JP.   +6 more
europepmc   +2 more sources

Biopanning data bank 2018: hugging next generation phage display [PDF]

Database, 2018
AbstractThe 2018 update of the biopanning data bank (BDB) stores phage display data sequenced by Sanger sequencing and next generation sequencing technologies. In this work, we upgraded the database with more biopanning data sets and several new features, including (i) incorporation of next generation biopanning data and the unselected population where
Bifang He, Lixu Jiang, Yaocong Duan, Guoshi Chai, Yewei Fang, Juanjuan Kang, Min Yu, Ning Li, Zhongjie Tang, Pengcheng Yao, Pengcheng Wu, Ratmir Derda, Jian Huang 0004   +12 more
openaire   +3 more sources

A Beautiful Bind: Phage Display and the Search for Cell-Selective Peptides [PDF]

Viruses
Phage display has advanced the discovery of peptides that selectively bind to a wide variety of cell surface molecules, offering new modalities to modulate disease-related protein–protein interactions (PPIs).
Babak Bakhshinejad, Saeedeh Ghiasvand
doaj   +2 more sources

Biopanning of Antibody-Phage Clones Using Immunoplates Coated with Gel Slices of Electrophoresis: Immunogel-Biopanning

Biological and Pharmaceutical Bulletin, 2007
Biopanning of a phage library using a Western blotting membrane is difficult because of high background binding. We propose a reliable biopanning method, namely, immunogel-biopanning, which is performed using immunoplates coated with a molecular species fractionated from a crude sample by native polyacrylamide gel electrophoresis (PAGE).
Shunsuke Uchida, Shuhei Hashiguchi, Yuji Itô   +2 more
exaly   +4 more sources

3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders

BioTechniques
Phage display combined with biopanning is a powerful method for the discovery of therapeutic antibodies. Classic biopanning is lengthy and labor-intensive, requiring at least five days of work.
Samson Lichtenstein, Morgane Valles, Michael Mullin, Aleardo Morelli   +3 more
doaj   +2 more sources