Results 11 to 20 of about 3,391 (172)

3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders

BioTechniques
Phage display combined with biopanning is a powerful method for the discovery of therapeutic antibodies. Classic biopanning is lengthy and labor-intensive, requiring at least five days of work.
Samson Lichtenstein, Morgane Valles, Michael Mullin, Aleardo Morelli   +3 more
doaj   +3 more sources

Peptide Receptor-Functionalized AuNP-Embedded Zwitterionic Biopolymeric Nanohydrogel for Electrochemical Sclerostin Sensing. [PDF]

Adv Sci (Weinh)
We report an electrochemical biosensor that employs biopanning‐derived affinity peptides as selective receptors on a multifunctional chitosan–zwitterionic–gold nanoparticle (CS–ZI–AuNPs) nanohydrogel interface. The CS–ZI–AuNPs nanohydrogel exhibits antifouling properties, abundant sites for stable peptide immobilization, and efficient electrochemical ...
Yang HJ, Shin JH, Jo M, Lee C, Yi H, Ali ABH, Park JP.   +6 more
europepmc   +2 more sources

Rare antibody phage isolation and discrimination (RAPID) biopanning enables identification of high-affinity antibodies against challenging targets

Communications Biology, 2023
In vitro biopanning platforms using synthetic phage display antibody libraries have enabled the identification of antibodies against antigens that were once thought to be beyond the scope of immunization.
Dong hee Chung, Sophie Kong, Nicholas J. Young, Shih-Wei Chuo, Jamie V. Shiah, Emily J. Connelly, Peter J. Rohweder, Alexandra Born, Aashish Manglik, Jennifer R. Grandis, Daniel E. Johnson, Charles S. Craik   +11 more
doaj   +1 more source

Development of an Immunoassay Detection System for Koi Herpesvirus Using Recombinant Single-Chain Variable Fragments

Fishes, 2022
Koi herpesvirus (KHV) is a highly contagious virus that causes high mortality in koi and common carp, leading to a reduction in production worldwide.
Haneul Seo, Andre Ditya Maulana Lubis, Tae-Jin Choi, Tae-Sung Jung, Taek-Kyun Lee, Sukchan Lee   +5 more
doaj   +1 more source

Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing

Viruses, 2022
The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool.
Ane Beth Sloth, Babak Bakhshinejad, Malte Jensen, Camilla Stavnsbjerg, Mikkel Baldtzer Liisberg, Maria Rossing, Andreas Kjaer   +6 more
doaj   +1 more source

BDB: biopanning data bank [PDF]

Nucleic Acids Research, 2015
The BDB database (http://immunet.cn/bdb) is an update of the MimoDB database, which was previously described in the 2012 Nucleic Acids Research Database issue. The rebranded name BDB is short for Biopanning Data Bank, which aims to be a portal for biopanning results of the combinatorial peptide library. Last updated in July 2015, BDB contains 2904 sets
Bifang He, Guoshi Chai, Yaocong Duan, Zhiqiang Yan, Liuyang Qiu, Huixiong Zhang, Zechun Liu, Qiang He, Ke Han, Beibei Ru, Feng-Biao Guo, Hui Ding 0005, Hao Lin 0001, Xianlong Wang 0002, Nini Rao, Peng Zhou 0003, Jian Huang 0004   +16 more
openaire   +2 more sources

A Competitive Panning Method Reveals an Anti-SARS-CoV-2 Nanobody Specific for an RBD-ACE2 Binding Site

Vaccines, 2023
Most neutralizing antibodies neutralize the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by directly blocking the interactions between the spike glycoprotein receptor-binding domain (RBD) and its receptor, human angiotensin-converting ...
Siqi He, Jiali Wang, Hanyi Chen, Zhaohui Qian, Keping Hu, Bingjie Shi, Jianxun Wang   +6 more
doaj   +1 more source

Biopanning the mimotopes of aflatoxin B1 and their immunogenicity [PDF]

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020
RESUMO Utilizando um anticorpo monoclonal contra a aflatoxina B1 (AFB1) como ligante, foi identificado um mimotopo específico de aflatoxina B1 após se realizarem quatro ciclos de seleção biológica de 7-peptídeos aleatórios em biblioteca de fago exibida. O mimotopo é denominado P10, e sua sequência de aminoácidos é YRRHEKD.
J. Zhou, F. Chen, J. Li, C. Li, Y. Qi, H. Liu, G. Zhang, A. Wang   +7 more
openaire   +3 more sources

Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women

mSphere, 2020
Chlamydia trachomatis is an obligate intracellular bacterium. C. trachomatis infection is the most prevalent bacterial sexually transmitted infection and can lead to pelvic inflammatory disease and infertility in women. There is no licensed vaccine for C.
Amanda L. Collar, Alexandria C. Linville, Susan B. Core, Cosette M. Wheeler, William M. Geisler, David S. Peabody, Bryce Chackerian, Kathryn M. Frietze   +7 more
doaj   +1 more source

Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection

Biosensors, 2021
C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production,
Sandra Oloketuyi, Robert Bernedo, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziółka-Jönsson, Katarzyna Szot-Karpińska, Harald Kolmar, Ario de Marco   +9 more
doaj   +1 more source