Results 31 to 40 of about 4,252 (213)

BDB: biopanning data bank [PDF]

Nucleic Acids Research, 2015
The BDB database (http://immunet.cn/bdb) is an update of the MimoDB database, which was previously described in the 2012 Nucleic Acids Research Database issue. The rebranded name BDB is short for Biopanning Data Bank, which aims to be a portal for biopanning results of the combinatorial peptide library. Last updated in July 2015, BDB contains 2904 sets
Bifang He, Guoshi Chai, Yaocong Duan, Zhiqiang Yan, Liuyang Qiu, Huixiong Zhang, Zechun Liu, Qiang He, Ke Han, Beibei Ru, Feng-Biao Guo, Hui Ding 0005, Hao Lin 0001, Xianlong Wang 0002, Nini Rao, Peng Zhou 0003, Jian Huang 0004   +16 more
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Biopanning the mimotopes of aflatoxin B1 and their immunogenicity [PDF]

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2020
RESUMO Utilizando um anticorpo monoclonal contra a aflatoxina B1 (AFB1) como ligante, foi identificado um mimotopo específico de aflatoxina B1 após se realizarem quatro ciclos de seleção biológica de 7-peptídeos aleatórios em biblioteca de fago exibida. O mimotopo é denominado P10, e sua sequência de aminoácidos é YRRHEKD.
J. Zhou, F. Chen, J. Li, C. Li, Y. Qi, H. Liu, G. Zhang, A. Wang   +7 more
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Antibodies to Variable Domain 4 Linear Epitopes of the Chlamydia trachomatis Major Outer Membrane Protein Are Not Associated with Chlamydia Resolution or Reinfection in Women

mSphere, 2020
Chlamydia trachomatis is an obligate intracellular bacterium. C. trachomatis infection is the most prevalent bacterial sexually transmitted infection and can lead to pelvic inflammatory disease and infertility in women. There is no licensed vaccine for C.
Amanda L. Collar, Alexandria C. Linville, Susan B. Core, Cosette M. Wheeler, William M. Geisler, David S. Peabody, Bryce Chackerian, Kathryn M. Frietze   +7 more
doaj   +1 more source

A Competitive Panning Method Reveals an Anti-SARS-CoV-2 Nanobody Specific for an RBD-ACE2 Binding Site

Vaccines, 2023
Most neutralizing antibodies neutralize the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by directly blocking the interactions between the spike glycoprotein receptor-binding domain (RBD) and its receptor, human angiotensin-converting ...
Siqi He, Jiali Wang, Hanyi Chen, Zhaohui Qian, Keping Hu, Bingjie Shi, Jianxun Wang   +6 more
doaj   +1 more source

Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection

Biosensors, 2021
C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production,
Sandra Oloketuyi, Robert Bernedo, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziółka-Jönsson, Katarzyna Szot-Karpińska, Harald Kolmar, Ario de Marco   +9 more
doaj   +1 more source

Quantitative PCR of T7 Bacteriophage from Biopanning [PDF]

Journal of Visualized Experiments, 2018
This protocol describes the use of quantitative PCR (qPCR) to enumerate T7 phages from phage selection experiments (i.e., "biopanning"). qPCR is a fluorescence-based approach to quantify DNA, and here, it is adapted to quantify phage genomes as a proxy for phage particles.
Xiujuan, Peng, Jasmim, Leal, Rashmi, Mohanty, Melissa, Soto, Debadyuti, Ghosh   +4 more
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Biopanning of allergens from wasp sting patients [PDF]

Bioscience Reports, 2018
Objective: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom. Methods: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 ...
Chai, Lin, Yang, Xianyi, Liu, Mei, Liu, Chunyan, Han, Limei, Guo, Hui, Li, Changsheng, Sun, Yuwen, Li, Xiaoyan, Xiao, Min, Fang, Zhicheng   +10 more
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An overview on application of phage display technique in immunological studies

Asian Pacific Journal of Tropical Biomedicine, 2017
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application ...
Abbas Rami, Mahdi Behdani, Najmeh Yardehnavi, Mahdi Habibi-Anbouhi, Fatemeh Kazemi-Lomedasht   +4 more
doaj   +1 more source

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)

Frontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda, Tatsuhiko Ozawa, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki   +13 more
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The Application of Next Generation Sequencing in Phage Display: A Short Review [PDF]

ImmunoAnalysis, 2021
Phage display is a method of studying protein-protein, protein-peptide, and protein-DNA that uses bacteriophages to bind proteins with genetic information that encodes them.
Ali Aghebati-Maleki, Behzad Baradaran, Khalil Hajiasgharzadeh, Babak Bakhshinejad, leili Aghebati-Maleki   +4 more
doaj   +1 more source