Results 21 to 30 of about 3,391 (172)

Quantitative PCR of T7 Bacteriophage from Biopanning [PDF]

Journal of Visualized Experiments, 2018
This protocol describes the use of quantitative PCR (qPCR) to enumerate T7 phages from phage selection experiments (i.e., "biopanning"). qPCR is a fluorescence-based approach to quantify DNA, and here, it is adapted to quantify phage genomes as a proxy for phage particles.
Xiujuan, Peng, Jasmim, Leal, Rashmi, Mohanty, Melissa, Soto, Debadyuti, Ghosh   +4 more
openaire   +2 more sources

VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)

Frontiers in Bioengineering and Biotechnology, 2023
The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda, Tatsuhiko Ozawa, Tatsuhiko Ozawa, Hiroki Zenke, Yoh Ohnuki, Yuri Umeda, Wei Zhou, Honoka Tomoda, Akihiko Takechi, Kimiyoshi Narita, Takaaki Shimizu, Takuya Miyakawa, Yuji Ito, Tatsuya Sawasaki   +13 more
doaj   +1 more source

Biopanning of allergens from wasp sting patients [PDF]

Bioscience Reports, 2018
Objective: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom. Methods: Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 ...
Chai, Lin, Yang, Xianyi, Liu, Mei, Liu, Chunyan, Han, Limei, Guo, Hui, Li, Changsheng, Sun, Yuwen, Li, Xiaoyan, Xiao, Min, Fang, Zhicheng   +10 more
openaire   +2 more sources

An overview on application of phage display technique in immunological studies

Asian Pacific Journal of Tropical Biomedicine, 2017
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application ...
Abbas Rami, Mahdi Behdani, Najmeh Yardehnavi, Mahdi Habibi-Anbouhi, Fatemeh Kazemi-Lomedasht   +4 more
doaj   +1 more source

Mimotopes selected by biopanning with high-titer HIV-neutralizing antibodies in plasma from Chinese slow progressors

Brazilian Journal of Infectious Diseases, 2012
OBJECTIVE: One approach to identifying HIV-1 vaccine candidates is to dissect the natural antiviral immune response in treatment-naïve individuals infected for over ten years, considered slow progressor patients (SPs).
Xiaoli Zhang, Xiaoxu Han, Di Dai, Bao Mingjia, Zining Zhang, Min Zhang, Tristan Bice, Min Zhao, Yaming Cao, Hong Shang   +9 more
doaj   +3 more sources

Mimotopes selected by biopanning with high-titer HIV-neutralizing antibodies in plasma from Chinese slow progressors

Brazilian Journal of Infectious Diseases, 2012
OBJECTIVE: One approach to identifying HIV-1 vaccine candidates is to dissect the natural antiviral immune response in treatment-naïve individuals infected for over ten years, considered slow progressor patients (SPs).
Xiaoli Zhang, Xiaoxu Han, Di Dai, Bao Mingjia, Zining Zhang, Min Zhang, Tristan Bice, Min Zhao, Yaming Cao, Hong Shang   +9 more
doaj   +1 more source

The Application of Next Generation Sequencing in Phage Display: A Short Review [PDF]

ImmunoAnalysis, 2021
Phage display is a method of studying protein-protein, protein-peptide, and protein-DNA that uses bacteriophages to bind proteins with genetic information that encodes them.
Ali Aghebati-Maleki, Behzad Baradaran, Khalil Hajiasgharzadeh, Babak Bakhshinejad, leili Aghebati-Maleki   +4 more
doaj   +1 more source

Phage Display Derived Monoclonal Antibodies: From Bench to Bedside

Frontiers in Immunology, 2020
Monoclonal antibodies (mAbs) have become one of the most important classes of biopharmaceutical products, and they continue to dominate the universe of biopharmaceutical markets in terms of approval and sales.
Mohamed A. Alfaleh, Mohamed A. Alfaleh, Hashem O. Alsaab, Ahmad Bakur Mahmoud, Almohanad A. Alkayyal, Martina L. Jones, Martina L. Jones, Stephen M. Mahler, Stephen M. Mahler, Anwar M. Hashem, Anwar M. Hashem   +10 more
doaj   +1 more source

Characterization of the Binding Behavior of Specific Cobalt and Nickel Ion-Binding Peptides Identified by Phage Surface Display

Separations, 2022
In recent years, the application focus of phage surface display (PSD) technology has been extended to the identification of metal ion-selective peptides. In previous studies, two phage clones—a nickel-binding one with the peptide motif CNAKHHPRCGGG and a
Sabine Matys, Lisa-Marie Morawietz, Franziska Lederer, Katrin Pollmann   +3 more
doaj   +1 more source

Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface

Brazilian Journal of Medical and Biological Research, 2000
We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed.
A.Q. Maranhão, M.M. Brígido
doaj   +1 more source