Results 21 to 30 of about 4,252 (213)
Specific Blocking to Improve Biopanning in Biological Samples such as Serum and Hybridoma Supernatants [PDF]
BioTechniques, 2001 Screening phage-displayed peptide libraries (biopanning) is an important technique for acquiring peptide ligands and for mapping peptide epitopes recognized by antibodies (Ab). In biological samples, other materials, not only contaminants but also natural constituents, often interfere with biopanning.
Bradley T. Messmer, David S. Thaler
openaire +4 more sources
Screening of Peptide Sequences with Affinity to Bisphenol A by Biopanning [PDF]
Korean Journal of Microbiology, 2013 Bisphenol A (BPA) is a highly hazardous component to human since it is regarded as one of endocrine disruptors. For the analysis and/or removal of BPA, the searching for the specific ligand with a selective affinity to target BPA is required. In order to find the peptide moiety that specifically binds to BPA, the ultrasound-assisted biopanning was ...
Ik-Keun Yoo, Woo-Seok Choe
exaly +2 more sources
On the origin of non-specific binders isolated in the selection of phage display peptide libraries [PDF]
Frontiers in MicrobiologyOver the recent decades, phage display has been used successfully to identify a variety of peptides with diagnostic and therapeutic applications. Despite the significant role of this technology in the pharmaceutical industry, the affinity selection of ...
Babak Bakhshinejad +3 more
doaj +2 more sources
Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands [PDF]
Methods in Molecular Biology, 2009 One limitation in the development of biosensors for the early detection of disease is the availability of high specificity and affinity ligands for biomarkers that are indicative of a pathogenic process. Within the past 10 years, biopanning of phage displayed peptide libraries on intact cells has proven to be a successful route to the identification of
Michael J Mcguire +2 more
exaly +3 more sources
Examining the Interactome of Huperzine A by Magnetic Biopanning
PLoS ONE, 2012 Huperzine A is a bioactive compound derived from traditional Chinese medicine plant Qian Ceng Ta (Huperzia serrata), and was found to have multiple neuroprotective effects. In addition to being a potent acetylcholinesterase inhibitor, it was thought to act through other mechanisms such as antioxidation, antiapoptosis, etc.
Wei Guo +8 more
openaire +5 more sources
Alpseq: an open-source workflow to turbocharge nanobody discovery with high-throughput sequencing [PDF]
mAbsNanobodies have emerged as promising tools for many biotechnological applications due to their small size, high stability and remarkable binding specificity.
Kathleen Zeglinski +9 more
doaj +2 more sources
Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species. [PDF]
PLoS ONE, 2013 The objective of this study was to produce phage display-derived binders with the ability to distinguish Listeria monocytogenes from other Listeria spp., which may have potential utility to enhance detection of Listeria monocytogenes.
Josephine Morton +5 more
doaj +1 more source
Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5
Molecules, 2021 WD40 is a ubiquitous domain presented in at least 361 human proteins and acts as scaffold to form protein complexes. Among them, WDR5 protein is an important mediator in several protein complexes to exert its functions in histone modification and ...
Jiawen Cao +10 more
doaj +1 more source
Fishes, 2022 Koi herpesvirus (KHV) is a highly contagious virus that causes high mortality in koi and common carp, leading to a reduction in production worldwide.
Haneul Seo +5 more
doaj +1 more source
Viruses, 2022 The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool.
Ane Beth Sloth +6 more
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