Results 41 to 50 of about 3,391 (172)

Development of an Anti-Zearalenone Nanobody Phage Display Library and Preparation of Specific Nanobodies

Current Issues in Molecular Biology
Zearalenone (ZEN), a toxic estrogenic mycotoxin in cereals, threatens human and animal health through reproductive, immune, and cytotoxic effects, necessitating sensitive detection methods.
Ying Zeng, Yiying Hu, Ganying Chen, Qingqing Feng, Ruiting Wang, Zhilin Zhang, Jinxian Chen, Junbin Liao, Danrong Lin, Wei Zhu   +9 more
doaj   +1 more source

Progress on Phage Display Technology: Tailoring Antibodies for Cancer Immunotherapy

Viruses, 2023
The search for innovative anti-cancer drugs remains a challenge. Over the past three decades, antibodies have emerged as an essential asset in successful cancer therapy.
Renato Kaylan Alves França, Igor Cabral Studart, Marcus Rafael Lobo Bezerra, Larissa Queiroz Pontes, Antonio Marcos Aires Barbosa, Marcelo Macedo Brigido, Gilvan Pessoa Furtado, Andréa Queiroz Maranhão   +7 more
doaj   +1 more source

Specific Blocking to Improve Biopanning in Biological Samples such as Serum and Hybridoma Supernatants [PDF]

BioTechniques, 2001
Screening phage-displayed peptide libraries (biopanning) is an important technique for acquiring peptide ligands and for mapping peptide epitopes recognized by antibodies (Ab). In biological samples, other materials, not only contaminants but also natural constituents, often interfere with biopanning.
Bradley T. Messmer, David S. Thaler
openaire   +3 more sources

Programmable Nanobody‐Targeting Chimeras Enable Intracellular Viral Protein Degradation

Advanced Science, EarlyView.
Nab‐TAC enables targeted degradation of HBV surface antigen (HBsAg) in vivo. By fusing nanobody‐based recognition domains with programmable proteasome‐recruiting degradation signals, Nab‐TAC reduces HBsAg levels in a hydrodynamic HBV mouse model. ABSTRACT Chronic hepatitis B virus (HBV) infection remains a major global health challenge, largely because
Max Yu‐Chen Pan, Ting‐Hui Lee, Tzu‐Ning Peng, Eva Yi‐Hsuan Wu, Yu‐Ting Chiu, Chih‐Chien Chiu, Hong Vinh Nguyen, Yuan‐Shao Pao, Ming‐Hong Chao, Richard Kuan‐Lin Lee, Han‐Chung Wu, Lily Hui‐Ching Wang   +11 more
wiley   +1 more source

Hybrid Macrocyclic Peptides — Synthetic Strategies to Diversify and Cyclize Peptide Libraries in Phage Display Selections

Chemistry – A European Journal, EarlyView.
Hybrid macrocyclic peptides unite genetically encoded peptide libraries with the structural complexity of synthetic small molecules. This Review critically analyzes phage‐compatible cyclization and diversification chemistries, highlights emerging opportunities beyond traditional cysteine‐based approaches, and outlines how future advances may enable the
Titia Rixt Oppewal, Clemens Mayer
wiley   +1 more source

Propagation Capacity of Phage Display Peptide Libraries Is Affected by the Length and Conformation of Displayed Peptide

Molecules, 2023
The larger size and diversity of phage display peptide libraries enhance the probability of finding clinically valuable ligands. A simple way of increasing the throughput of selection is to mix multiple peptide libraries with different characteristics of
Danna Kamstrup Sell, Anders Wilgaard Sinkjaer, Babak Bakhshinejad, Andreas Kjaer   +3 more
doaj   +1 more source

Nanobodies as next‐generation targeting platforms: From discovery technologies to translational biomedicine

VIEW, EarlyView.
Nanobodies, derived from the variable domains of camelid heavy‐chain‐only antibodies, have emerged as transformative biomedical tools due to their nanoscale size, exceptional stability, and unique capacity to recognize cryptic epitopes. This review provides a comprehensive overview of the field, outlining the structural and biochemical features of ...
Zhenrui Ye, Xianyang Li, Meixiao Zhan
wiley   +1 more source

Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy

Molecules, 2023
Nanobodies (Nbs) are single domain antibody fragments derived from heavy-chain antibodies found in members of the Camelidae family. They have become a relevant class of biomolecules for many different applications because of several important advantages ...
María Angélica Contreras, Yunier Serrano-Rivero, Alaín González-Pose, Julieta Salazar-Uribe, Marcela Rubio-Carrasquilla, Matheus Soares-Alves, Natalie C. Parra, Frank Camacho-Casanova, Oliberto Sánchez-Ramos, Ernesto Moreno   +9 more
doaj   +1 more source

Optimization of antibody binding by adjusting thermodynamic stability during the maturation of antibody affinities

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Somatic mutations and antibody clone selection occur in B‐cell hyper‐evolution over extremely brief periods of time. Herein, we developed a technique for antibody screening by immunizing alpacas with antigens and observing antibody sequence transitions over time, a method we named Tracking the Evolution of Antibodies over time.
Yoko Akazawa‐Ogawa, Tomonari Matsuda, Norihiko Kiyose, Nobuo Miyazaki, Tetsuo Fukuta, Motoyasu Adachi, Yuji Ito, Yoshihisa Hagihara   +7 more
wiley   +1 more source

High‐Throughput Isolation of Nanomouse‐Derived VHH Domains: A Practical Guide from Immunization to Nanobody Expression

Current Protocols, Volume 6, Issue 7, July 2026.
Abstract Nanobodies are small but specific heavy chain–only antibody fragments. Their small size, relative stability, and ability to access difficult to reach deep‐tissue antigens makes them valuable research, diagnostic, and therapeutic tools. Nanobodies are derived from the variable heavy (VH) domain of heavy chain–only antibodies that are unique to ...
Tessa J. Casselman, Asa W. Huffaker, Kristie C. Mitchell, Jamie E. Schnarrs, Sylvia Ni, Mary E. Skinner, Matthias C. Truttmann   +6 more
wiley   +1 more source