Results 41 to 50 of about 5,879 (222)

Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning

mAbs, 2023
Despite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated variants during biopanning.
Tomoyuki Ito, Thuy Duong Nguyen, Yutaka Saito, Yoichi Kurumida, Hikaru Nakazawa, Sakiya Kawada, Hafumi Nishi, Koji Tsuda, Tomoshi Kameda, Mitsuo Umetsu   +9 more
doaj   +1 more source

Production and characterization of specific monoclonal antibodies binding the Plasmodium falciparum diagnostic biomarker, histidine-rich protein 2 [PDF]

, 2014
BACKGROUND: Early and accurate diagnosis of Plasmodium falciparum infection is important for providing appropriate treatment to patients with malaria.
Chiuan Leow, James McCarthy, Martina Jones, Qin Cheng, Stephen Mahler   +4 more
core   +2 more sources

Identification of Functional mimotopes of human Vasorin Ectodomain by Biopanning [PDF]

International Journal of Biological Sciences, 2018
Human vasorin (VASN) as a type I transmembrane protein, is a potential biomarker of hepatocellular carcinoma, which could expedite HepG2 cell proliferation and migration significantly in vitro. The ectodomain of VASN was proteolytically released to generate soluble VASN (sVASN), which was validated to be the active form.
Li, Da, Zhang, Tan, Yang, Xiqin, Geng, Jie, Li, Shaohua, Ding, Hongmei, Li, Hui, Huang, Aixue, Wang, Chaonan, Sun, Leqiao, Bai, Chenjun, Zhang, Heqiu, Li, Jie, Dong, Jie, Shao, Ningsheng   +14 more
openaire   +2 more sources

Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites [PDF]

, 2012
The vast majority of anti-infective therapeutics on the market or in development are small molecules; however, there is now a nascent pipeline of biological agents in development.
Bishop-Hurley, Sharon L., Cooper, Matthew A., Huang, Johnny X.   +2 more
core   +1 more source

3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders

BioTechniques
Phage display combined with biopanning is a powerful method for the discovery of therapeutic antibodies. Classic biopanning is lengthy and labor-intensive, requiring at least five days of work.
Samson Lichtenstein, Morgane Valles, Michael Mullin, Aleardo Morelli   +3 more
doaj   +1 more source

Introduction of plasmid encoding for rare tRNAs reduces amplification bias in phage display biopanning

BioTechniques, 2015
Amplification bias is a major hurdle in phage display protocols because it imparts additional, unintended selection pressure beyond binding to the desired target.
Benjamin J. Umlauf, Michael J. McGuire, Kathlynn C. Brown   +2 more
doaj   +1 more source

Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries

Antibodies, 2022
A rapid and effective method to identify disease-specific antibodies from clinical patients is important for understanding autoimmune diseases and for the development of effective disease therapies.
Aric Huang, Wei Jin, Ahmed S. Fahad, Brooklyn K. Mussman, Grazia Paola Nicchia, Bharat Madan, Matheus Oliveira de Souza, J. Daniel Griffin, Jeffrey L. Bennett, Antonio Frigeri, Cory J. Berkland, Brandon J. DeKosky   +11 more
doaj   +1 more source

Inorganic nanowires [PDF]

, 2014
Provided in one embodiment is a method of forming an inorganic nanowire, comprising: providing an elongated organic scaffold; providing a plurality of inorganic nanoparticles attached to the organic scaffold along a length of the organic scaffold; and ...
Belcher, Angela M., Mao, Chuanbin, Solis, Daniel J.   +2 more
core   +1 more source

GPCR-Based Bioactive Peptide Screening Using Phage-Displayed Peptides and an Insect Cell System for Insecticide Discovery

Biomolecules, 2021
The discovery of new insecticides improves integrated pest management (IPM), but is usually a long high-risk process with a low probability of success.
Man-Yeon Choi, Robert K. Vander Meer
doaj   +1 more source

Screening and characterization of novel specific peptides targeting MDA-MB-231 claudin-low breast carcinoma by computer-aided phage display methodologies [PDF]

, 2016
Background: Claudin-low breast carcinoma represents 19% of all breast cancer cases and is characterized by an aggressive progression with metastatic nature and high rates of relapse.
Azeredo, Joana, Ferreira, Débora, Kluskens, Leon, Maria Suarez-Diez, Martins, Ivone M., Nóbrega, Franklin Luzia, Rodrigues, L. R.   +6 more
core   +1 more source