Results 61 to 70 of about 4,252 (213)

Development of an Anti-Zearalenone Nanobody Phage Display Library and Preparation of Specific Nanobodies

Current Issues in Molecular Biology
Zearalenone (ZEN), a toxic estrogenic mycotoxin in cereals, threatens human and animal health through reproductive, immune, and cytotoxic effects, necessitating sensitive detection methods.
Ying Zeng, Yiying Hu, Ganying Chen, Qingqing Feng, Ruiting Wang, Zhilin Zhang, Jinxian Chen, Junbin Liao, Danrong Lin, Wei Zhu   +9 more
doaj   +1 more source

Hierarchical Targeting of TREM2+ Myeloid Cells via Acid‐Triggered OMVs Reprogram Immunosuppression and Suppress Osteolysis in Bone‐Metastatic TNBC

Advanced Science, Volume 13, Issue 32, 9 June 2026.
This study developed a hierarchical targeting nanoplatform, siTREM2@ETP‐PEOz‐OMVs, against triple‐negative breast cancer (TNBC) bone metastasis. It precisely delivers therapeutic siTREM2 to monocytes/macrophages within the metastatic niche. This intervention dually regulates cell fate: reprogramming immunosuppressive macrophages and inhibiting ...
Fanglu Chen, Yucheng Xue, Xiao Ma, Liang Chen, Zhenxuan Shao, Hangxiang Sun, Haochen Mou, Fangqian Wang, Zilong Li, Yechao Shen, Zhaoming Ye, Xiaohua Yu, Shengdong Wang   +12 more
wiley   +1 more source

Enrichment of the phages in each round of biopanning.

, 2013
Enrichment of the phages in each round of biopanning.
Lili Wang (104605), Zhizhong Zhou (484432), Wei Zhang (405), Weina Li (484433), Yingqi Zhang (285021), Jing Yang (13969), Haichuan Su (484434), Yang Song (88132), Kuo Zhang (337825)   +8 more
core   +1 more source

Propagation Capacity of Phage Display Peptide Libraries Is Affected by the Length and Conformation of Displayed Peptide

Molecules, 2023
The larger size and diversity of phage display peptide libraries enhance the probability of finding clinically valuable ligands. A simple way of increasing the throughput of selection is to mix multiple peptide libraries with different characteristics of
Danna Kamstrup Sell, Anders Wilgaard Sinkjaer, Babak Bakhshinejad, Andreas Kjaer   +3 more
doaj   +1 more source

Monitoring the progress of biopanning by polyclonal phage ELISA

, 2015
. (A) The polyclonal phage mixture from each round of biopanning was tested for recognition of Gad m 1, Cyp c 1 and Onc m 1. (B) Monoclonal phage ELISA: Ten single phage clones randomly selected from each third round of biopanning were tested for binding
Klaus R. Liedl (109013), Daniela Ackerbauer (828235), Fábio C. L. Almeida (270100), Adolfo H. Moraes (828236), Nina Lengger (687992), Heimo Breiteneder (352019), Maria Kostadinova (828234), Christof Ebner (368198), Julian E. Fuchs (108999), Ana Paula Valente (132725), Merima Bublin (687993), Christine Hafner (828237), Christian Radauer (687991)   +12 more
core   +1 more source

Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, Volume 13, Issue 36, 29 June 2026.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

Expansion and Refinement of Deep Sequence-Coupled Biopanning Technology for Epitope-Specific Antibody Responses in Human Serum

Viruses, 2020
Identifying the specific epitopes targeted by antibodies elicited in response to infectious diseases is important for developing vaccines and diagnostics.
Nikole L. Warner, Alexandria C. Linville, Susan B. Core, Brechla Moreno, Juan M. Pascale, David S. Peabody, Bryce Chackerian, Kathryn M. Frietze   +7 more
doaj   +1 more source

Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy

Molecules, 2023
Nanobodies (Nbs) are single domain antibody fragments derived from heavy-chain antibodies found in members of the Camelidae family. They have become a relevant class of biomolecules for many different applications because of several important advantages ...
María Angélica Contreras, Yunier Serrano-Rivero, Alaín González-Pose, Julieta Salazar-Uribe, Marcela Rubio-Carrasquilla, Matheus Soares-Alves, Natalie C. Parra, Frank Camacho-Casanova, Oliberto Sánchez-Ramos, Ernesto Moreno   +9 more
doaj   +1 more source

NS1 and E enriched epitopes identified by deep sequence-coupled biopanning with patient serum after two rounds of biopanning.

, 2017
NS1 conservation (A) is shown for the highly enriched overlapping peptides from patient 1536 after two rounds of biopanning. Bold letters indicate the flanking amino acids that are solved in the crystal structure (see Fig 5F for structure).
Kathryn M. Frietze (3713686), Bryce Chackerian (66861), Brechla Moreno (2586703), David S. Peabody (298250), Juan M. Pascale (179353)   +4 more
core   +1 more source

Nanobodies: A Promising Toolkit for Diagnostic Applications

SmartMat, Volume 7, Issue 3, June 2026.
This review focuses on camelid‐derived nanobodies (VHHs) and explains how their small size and high stability support robust diagnostic design. Applications across ELISA, lateral flow assays, and PET/SPECT imaging are summarized, along with clinical progress such as caplacizumab.
Wei Wu, Abid Ali, Li Sun, Jiahao Hu, Lina Hameed, Saba Shafi, Ali Raza, Khuzin Dinislam, Wenjing Xing, Long Rong, Jie Lin, Jian Ma   +11 more
wiley   +1 more source