Protocol for Metatranscriptomic analysis of Intestinal Microbiota [PDF]
, 2012 The objective of this publication is to provide the detailed protocol for metartancriptomisc studies of animal intestinal microbiota. The protocol describes isolation of high quality microbial community RNA from the mammalian intestinal content ...
Alvaro Hernandez +4 more
core +2 more sources
cDNA cloning of MCT1, a monocarboxylate transporter from rat skeletal muscle [PDF]
, 1995 PCR was used to amplify the coding region of CHO MCT1 cDNA. This was then used to screen a rat skeletal muscle cDNA library which lead to the isolation of a full length cDNA encoding MCT1 from rat.
Halestrap, Andrew P. +2 more
core +1 more source
Human complement factor H [PDF]
, 1986 We isolated cDNA clones coding for the functionally important tryptic N-terminal38- kDa fragment of human complement control protein factor H using polyclonal and monoclonal antibodies to screen a human liver cDNA library cloned in a bacterial expression
Dierich, Manfred P. +4 more
core +1 more source
Screening of a HUVEC cDNA library with transplant-associated coronary artery disease sera identifies RPL7 as a candidate autoantigen associated with this disease. [PDF]
, 2001 A HUVEC cDNA library was screened with sera from two patients who had developed transplant-associated coronary artery disease (TxCAD) following cardiac transplantation.
Linke, A.T. +5 more
core +4 more sources
Construction of two Neurospora crassa Lambda ZapII-cDNA libraries [PDF]
, 1991 In the presence of the anti-microtubule agent benomyl, N. crassa synthesizes and secretes a major 60 kDa protein in the culture medium. This protein is absent in untreated cells (Rossier et al. 1989. Eur. J. Cell Biol. 50:333-339).
Hoang-Van, K., Rossier, C., Turian, G.
core +2 more sources
Isolation and characterization of a Coffea canephora ERF-like cDNA [PDF]
, 2005 ERFs (Ethylene-Responsive Element Binding Factors) are members of a transcription factors family unique to plants. They contain a well-conserved segment, which interacts specifically with sequences containing AGCCGCC motifs (GCC box) located in the ...
Bustamante-Porras, J. +4 more
core +2 more sources
cDNA that encodes active agrin [PDF]
, 1992 Agrin is thought to mediate the motor neuron-induced aggregation of AChRs and AChE on the surface of muscle fibers at neuromuscular junctions. We have isolated a cDNA from a chick brain library that, based on sequence homology and expression experiments,
Bolander +42 more
core +2 more sources
Construction and characterization of a cDNA library from human brain glioma cell line U251 with overexpressed exogenous p53 gene [PDF]
, 2013 The tumor-suppressor gene p53 and its downstream genes consist of a complicated gene network, and the challenge to understand the network is to identify p53 downstream genes.
Li, Yan-zhe +4 more
core +2 more sources
Development of a method for screening short-lived proteins using green fluorescent protein. [PDF]
, 2004 We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library
Coffino, Philip +2 more
core
A new 5′-non-coding region for human placental insulin-like growth factor II mRNA expression [PDF]
, 1987 A human placenta cDNA library was screened for insulin-like growth factor II (IGF-II). Four clones were selected, which exhibited an IGF-II cDNA coding sequence identical to those previously described for human adult liver IGF-II cDNA. Extensive sequence
Binoux, M. +5 more
core +1 more source