Results 11 to 20 of about 284,909 (310)

A Rapid Method for Analysis of cDNA Synthesis Using Ion-Pair Reversed-Phase High Performance Liquid Chromatography [PDF]

open access: yesJournal of Cell and Molecular Research, 2020
We have developed a rapid, quantitative method for analysing the outcome of the first strand synthesis step in cDNA library preparation, yield and molecular weight range of the final cDNA products are determined after size fractionation.
Maryam M. Matin, David P. Hornby
doaj   +1 more source

Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection

open access: yesBioTechniques, 2004
Phage display technologies are powerful tools for selecting binding ligands against purified molecular targets, live cells, and organ vasculature. However, the selection of natural ligands using phage display has been limited because of significant ...
Peggy Ho Faix   +5 more
doaj   +1 more source

Preparation of cDNA Library for dRNA-seq

open access: yesBio-Protocol, 2012
microRNAs (miRNAs) are ubiquitous regulators of gene expression in eukaryotic organisms, which guide Argonaute proteins (AGO) to cleave target mRNA or inhibit its translation based on sequence complementarity. In plants, miRNA directed cleavage occurs on
Feng Li, Barbara Baker
doaj   +1 more source

Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identification of Interacting Proteins with Brn1 in Curvularia lunata

open access: yesThe Plant Pathology Journal, 2015
Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated.
Jin-Xin Gao   +3 more
doaj   +1 more source

Cloning and sequence analysis of two calmodulin isoform cDNA from mulberry

open access: yes浙江大学学报. 农业与生命科学版, 2008
In order to elucidate the roles of calmodulin in mulberry stress-resistance, cDNA library of Fengchisang seedlings was constructed using SMART (switching mechanism at 5′ end of RNA transcript) cDNA library construction technology.
WANG Wei   +4 more
doaj   +1 more source

Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [PDF]

open access: yesKafkas Universitesi Veteriner Fakültesi Dergisi, 2018
Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in
Nehir OZDEMIR OZGENTURK   +9 more
doaj   +1 more source

EST projects in aquaculture: sea bass, red tuna and perch

open access: yesItalian Journal of Animal Science, 2010
The modern technologies used in aquaculture may improve fish production and quality and, at the same time, reduce environmental impact with benefits on the public perception of the industry.
G. Bernardini   +7 more
doaj   +1 more source

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

open access: yesJournal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN   +3 more
doaj   +1 more source

Immune-related transcriptome of Coptotermes formosanus Shiraki workers: the defense mechanism. [PDF]

open access: yesPLoS ONE, 2013
Formosan subterranean termites, Coptotermes formosanus Shiraki, live socially in microbial-rich habitats. To understand the molecular mechanism by which termites combat pathogenic microbes, a full-length normalized cDNA library and four Suppression ...
Abid Hussain   +5 more
doaj   +1 more source

Identification of some unknown transcripts from SSH cDNA library of buffalo follicular oocytes

open access: yesAnimal, 2013
A buffalo oocyte-specific subtracted cDNA library was constructed to identify exclusively or preferentially oocyte-expressed genes. The library represented an enriched population of transcripts obtained from oocytes of diverse ovarian follicular origin ...
S.K. Rajput   +7 more
doaj   +1 more source

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