Results 21 to 30 of about 2,006 (151)

Hydroxylation of N-acetylneuraminic Acid Influences the in vivo Tropism of N-linked Sialic Acid-Binding Adeno-Associated Viruses AAV1, AAV5, and AAV6

Frontiers in Medicine, 2021
Adeno-associated virus (AAV) vectors are promising candidates for gene therapy. However, a number of recent preclinical large animal studies failed to translate into the clinic. This illustrates the formidable challenge of choosing the animal models that
Estrella Lopez-Gordo, Alejandro Orlowski, Arthur Wang, Alan Weinberg, Susmita Sahoo, Thomas Weber, Thomas Weber   +6 more
doaj   +1 more source

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods [PDF]

Veterinary World, 2022
Background and Aim: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/ Cas9) system ...
Quynh Anh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Lanh Thi Kim Do, Takeshige Otoi   +8 more
doaj   +1 more source

Phenotypic and Genetic Characterization for Incompatible Cross-Match Cases in the Feline AB Blood Group System

Frontiers in Veterinary Science, 2021
The feline AB blood group system (blood types A, B, and AB) encoding the cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) gene is the most significant in transfusion medicine and hemolysis of the newborn for cats.
Yumiko Uno, Masami Yaguchi, Tasuku Kobayashi, Eri Onozawa, Kazuhiko Ochiai, Kazuhiko Ochiai, Karin Yoshida, Chihiro Nakamura, Chihiro Udagawa, Toshinori Omi, Toshinori Omi   +10 more
doaj   +1 more source

An In Silico Functional Analysis of Non-Synonymous Single-Nucleotide Polymorphisms of Bovine CMAH Gene and Potential Implication in Pathogenesis

Pathogens, 2023
The sugar molecule N-glycolylneuraminic acid (Neu5Gc) is one of the most common sialic acids discovered in mammals. Cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH) catalyses the conversion of N-acetylneuraminic acid (Neu5Ac) to Neu5Gc ...
Oluwamayowa Joshua Ogun, Opeyemi S. Soremekun, Georg Thaller, Doreen Becker   +3 more
doaj   +1 more source

Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy. [PDF]

PLoS ONE, 2018
Cardiac failure is a major cause of mortality in patients with Duchenne muscular dystrophy (DMD). Antisense-mediated exon skipping has the ability to correct out-of-frame mutations in DMD to produce truncated but functional dystrophin.
Alison M Blain, Elizabeth Greally, Graham McClorey, Raquel Manzano, Corinne A Betts, Caroline Godfrey, Liz O'Donovan, Thibault Coursindel, Mike J Gait, Matthew J Wood, Guy A MacGowan, Volker W Straub   +11 more
doaj   +1 more source

Donor-Derived Cell-Free DNA (dd-cfDNA) as an Early Noninvasive Biomarker of Graft Injury in Pig-to-Monkey Islet Xenotransplantation. [PDF]

Xenotransplantation
ABSTRACT Background In pig‐to‐nonhuman primate islet transplantation, reliable, sensitive biomarkers are needed to detect graft damage at an early stage before irreversible islet loss occurs. In our study, we investigated donor‐derived cell‐free DNA (dd‐cfDNA) as an early, noninvasive biomarker of graft injury by analyzing its correlation with porcine ...
Yan JJ, Kim JM, Cho SI, Kim H, Kwak K, Kim H, Oh EJ, Yang J, Park CG, Jeong JC, Kim BS.   +10 more
europepmc   +2 more sources

Early Experimental Report of the First 8-Gene-Edited Pig-to-Rhesus Macaque Cardiac Xenotransplantation in China. [PDF]

Xenotransplantation
ABSTRACT Objectives This study aimed to investigate the functional recovery of an 8‐gene‐edited pig heart after orthotopic xenotransplantation, the characteristics of the recipient's immune responses, and the efficacy of postoperative complication management to provide comprehensive experimental evidence for the clinical translation of xenogeneic ...
Wang X, Wu X, Dai Z, Yan L, Yan J, He D, Li S, Ren Z, Zhang G, Cui G, Li X, Li X, Guan Y, Pan G, Yun IJ, Zhou W, Pan D, Li D.   +17 more
europepmc   +2 more sources

Porcine kidney xenotransplantation: From primate models to clinical reality. [PDF]

Animal Model Exp Med
In the face of a critical shortage of human donor kidneys for end‐stage renal disease patients, porcine kidney xenotransplantation has emerged as a viable solution. This field has navigated major hurdles, including immune rejection, physiological incompatibilities, potential biomechanical differences and the risk of cross‐species infection. To overcome
Guo Z, Zhang L, Deng S, Qin C.
europepmc   +2 more sources

An Overview of the Importance and Value of Porcine Species in Sialic Acid Research

Biology, 2022
Humans frequently interact with pigs, whose meat is also one of the primary sources of animal protein. They are one of the main species at the center of sialic acid (Sia) research.
Oluwamayowa Joshua Ogun, Georg Thaller, Doreen Becker   +2 more
doaj   +1 more source

Improved ESI-MS Sensitivity via an Imidazolium Tag (DAPMI-ITag) for Precise Sialic Acid Detection in Human Serum and CMAH-Null Mouse Tissues. [PDF]

Anal Chem
Sialic acids (Sias), consisting primarily of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), play crucial roles in many biological processes. The detection and quantification of Sias are essential for understanding their roles in
Zhang YY, Hu ZX, Zhang SY, Liu L, Galan MC, Voglmeir J, Ghirardello M.   +6 more
europepmc   +2 more sources