Results 141 to 150 of about 3,192 (173)
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Multifunctional deadenylase complexes diversify mRNA control
Nature Reviews Molecular Cell Biology, 2008Dynamic changes of the lengths of mRNA poly(A) tails are catalysed by diverse deadenylase enzymes. Modulating the length of the poly(A) tail of an mRNA is a widespread means of controlling protein production and mRNA stability. Recent insights illuminate the specialized activities, biological functions and regulation of deadenylases.
Aaron C Goldstrohm +2 more
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Regulation of the multisubunit CCR4-NOT deadenylase in the initiation of mRNA degradation
Current Opinion in Structural Biology, 2022The conserved CCR4-NOT complex initiates the decay of mRNAs by catalyzing the shortening of their poly(A) tails in a process known as deadenylation. Recent studies have provided mechanistic insights into the action and regulation of this molecular machine. The two catalytic enzymatic subunits of the complex hydrolyze polyadenosine RNA. Notably, the non-
Tobias Raisch, Eugene Valkov
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Biophysical and biochemical characterization of recombinant human Pop2 deadenylase
Protein Expression and Purification, 2008Pop2, a component of the Ccr4-Not complex, functions as a deadenylase both in vitro and in vivo. In this research, we found that the recombinant human Pop2 (hPop2) mainly existed in a compact monomeric state with a a + b tertiary structure type. The percentages of the secondary structures evaluated from the CD spectrum were about 37% a-helix, 14% b ...
Wei-Feng Liu, Yong-Bin Yan
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Dissecting the Role of the Ccr4–Not Deadenylase Complex in Pluripotency and Differentiation
2023In this chapter, we describe methods and assays to examine the role of the Ccr4-Not mRNA deadenylase complex in regulating mouse embryonic stem cell (ESC) pluripotency and differentiation. We present the following procedures: sgRNA design and cloning, the culture of mouse ESCs, transfection and colony picking, genotyping, generation of Cnot3 ...
Xiukun, Wang +3 more
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NOC out the fat: A short review of the circadian deadenylase Nocturnin
Annals of Medicine, 2008 The circadian clock is a conserved internal timekeeping mechanism that controls many aspects of physiology and behavior via the rhythmic expression of many genes. One of these rhythmic genes, Nocturnin, encodes a deadenylase--a ribonuclease that specifically removes the poly(A) tails from mRNAs.
Carla B Green
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Activity and Function of Deadenylases
2012Shortening of the poly(A) tail is the first and often rate-limiting step in mRNA degradation. Three poly(A)-specific 3' exonucleases have been described that can carry out this reaction: PAN, composed of two subunits; PARN, a homodimer; and the CCR4-NOT complex, a heterooligomer that contains two catalytic subunits and may have additional functions in ...
Christiane, Harnisch +4 more
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PAN-orama: three convergent views of a eukaryotic deadenylase
Nature Structural & Molecular Biology, 2014Post-transcriptional mRNA regulation is often attained by lengthening or shortening the 3′ poly(A) tail of a transcript. Eukaryotic mRNAs show a spectrum of deadenylation rates, thus allowing intricate control of gene expression, but the mechanisms that determine such rates are unclear.
Sophie, Martin, Jeff, Coller
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Characterization of the ubiquitin binding properties of the deadenylase Pan2
The FASEB Journal, 2016Messenger RNA (mRNA) stability plays an important role in regulating gene expression in eukaryotic cells. While a number of endo‐ and exo‐nucleolytic enzymes act on mRNA, removal of the poly(A) tail, or deadenylation, is often the first and rate‐limiting step in mRNA decay.
Zachary Swinney +3 more
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GW182 Proteins Directly Recruit Cytoplasmic Deadenylase Complexes to miRNA Targets [PDF]
Molecular Cell, 2011 miRNAs are posttranscriptional regulators of gene expression that associate with Argonaute and GW182 proteins to repress translation and/or promote mRNA degradation. miRNA-mediated mRNA degradation is initiated by deadenylation, although it is not known whether deadenylases are recruited to the mRNA target directly or by default, as a consequence of a ...
Joerg E Braun +2 more
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Nocturnin is a Circadian-Regulated Deadenylase
2017A hallmark of circadian clock components is their rhythmic expression profile. Nocturnin, a rhythmically expressed gene, was first identified in the Xenopus laevis retina. Since then, nocturnin homologues have been identified in several other organisms, including mouse, where it is also expressed with a high amplitude circadian rhythmicity.
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