Results 81 to 90 of about 3,096 (155)

Regulation of the Drosophila transcriptome by Pumilio and CCR4-NOT deadenylase

, 2023
ABSTRACTThe sequence-specific RNA-binding protein Pumilio controls development ofDrosophila; however, the network of mRNAs that it regulates remains incompletely characterized. In this study, we utilize knockdown and knockout approaches coupled with RNA-Seq to measure the impact of Pumilio on the transcriptome ofDrosophilacells.
Rebecca J. Haugen, Catherine Barnier, Nathan D. Elrod, Hua Luo, Madeline K. Jensen, Ping Ji, Craig A. Smibert, Howard D. Lipshitz, Eric J. Wagner, P. Lydia Freddolino, Aaron C. Goldstrohm   +10 more
openaire   +2 more sources

RNF187 Facilitates Proliferation and Migration of Human Spermatogonial Stem Cells Through WDR77 Polyubiquitination

Cell Proliferation, Volume 58, Issue 10, October 2025.
RNF187 mediates the polyubiquitination and subsequent degradation of WDR77, reduces H4R3me2s catalysed by the WDR77/PRMT5 complex, and derepresses the transcription of EGR1, thereby promoting the proliferation and migration of human SSCs. ABSTRACT The E3 ubiquitin ligase RNF187, also known as RING domain AP1 coactivator‐1, is a member of the RING ...
Haoyue Hu, Xiaoxue Xi, Bing Jiang, Kehan Wang, Tiantian Wu, Xia Chen, Yueshuai Guo, Tao Zhou, Xiaoyan Huang, Jun Yu, Tingting Gao, Yibo Wu, Bo Zheng   +12 more
wiley   +1 more source

Role of CPEBs in Learning and Memory

Journal of Neurochemistry, Volume 169, Issue 9, September 2025.
SUMOylation of CPEB3 and its association with target mRNA can lead to phase separation and localisation to P bodies, where translation of target mRNAs is repressed. (1) Activation of AMPA and NMDA receptors leads to (2) long‐term potentiation (LTP). Increased cellular Ca2+ concentration leads to activation of calpain‐2 and some proteolysis of CPEB3. (3)
David A. Hicks, Liam D. Aubrey, Jessica C. F. Kwok, Sheena E. Radford   +3 more
wiley   +1 more source

Plasmodium male gametocyte development and transmission are critically regulated by the two putative deadenylases of the CAF1/CCR4/NOT complex.

PLoS Pathogens, 2019
With relatively few known specific transcription factors to control the abundance of specific mRNAs, Plasmodium parasites may rely more on the regulation of transcript stability and turnover to provide sufficient gene regulation.
Kevin J Hart, Jenna Oberstaller, Michael P Walker, Allen M Minns, Mark F Kennedy, Ian Padykula, John H Adams, Scott E Lindner   +7 more
doaj   +1 more source

Interaction of antiproliferative protein Tob with the CCR4‐NOT deadenylase complex

Cancer Science, 2008
Tob protein, when overexpressed, suppresses growth of NIH3T3 cells, presumably by regulating expression of various growth‐related genes. However, the molecular mechanisms underlying Tob‐mediated regulation of gene expression have been obscure. To address this issue we established stable Tob‐expressing cell lines and used a proteomics approach to ...
Takashi, Miyasaka, Masahiro, Morita, Kentaro, Ito, Toru, Suzuki, Hiroyuki, Fukuda, Shizu, Takeda, Jun-Ichiro, Inoue, Kentaro, Semba, Tadashi, Yamamoto   +8 more
openaire   +2 more sources

PABPN1 Couples the Polyadenylation and Translation of Maternal Transcripts to Mouse Oocyte Meiotic Maturation

Advanced Science, Volume 12, Issue 23, June 20, 2025.
This study reveals that cytoplasmic PABPN1 is essential for mouse oocyte meiotic maturation by coordinating polyadenylation, translation, and degradation of maternal mRNAs. Pabpn1 knockout disrupts CDK1 activation, spindle formation, and chromosome alignment by impairing maturation‐promoting factor (MPF) regulation and BTG4‐mediated deadenylation ...
Xing‐Xing Dai, Yu‐Ke Wu, Shuai‐Bo Pi, Feng‐Jie Hu, Yun‐Wen Wu, Hang Qi, Zhiyi Li, Zhi‐Yan Jiang, Long‐Wen Zhao, Heng‐Yu Fan   +9 more
wiley   +1 more source

Mechanisms governing poly(A)-tail-length specificity of the human PAN2-PAN3 deadenylase complex

Cell Reports
Summary: The lifespan of most eukaryotic mRNAs is modulated by the gradual shortening of the poly(A) tail and removal of the associated poly(A)-binding protein.
Jana C. Albrecht, Timo Reitinger, Jérôme Basquin, Steffen Schüssler, Margot Riggi, Ingmar B. Schäfer, Elena Conti   +6 more
doaj   +1 more source

Insights into the structure and architecture of the CCR4-NOT complex

Frontiers in Genetics, 2014
The CCR4-NOT complex is a highly conserved, multifunctional machinery with a general role in controlling mRNA metabolism. It has been implicated in a number of different aspects of mRNA and protein expression, including mRNA degradation, transcription ...
Kun eXu, Yuwei eBai, Aili eZhang, Qionglin eZhang, Mark Gerrard Bartlam   +4 more
doaj   +1 more source

Targeting Zfp36 to combat cardiac hypertrophy: Insights into ferroptosis pathways

Clinical and Translational Medicine, Volume 15, Issue 3, March 2025.
Zinc finger protein 36 bounds to the 3′ untranslated region of Ythdc2 mRNA, facilitating its degradation. Ythdc2 can bind to SLC7A11 mRNA to accelerate its decay, thereby reducing glutathione levels and aggravating ferroptosis‐induced cardiac hypertrophy.
Mingyu Zhang, Xiaoxiang Guan, Zheng Dong, Chenxu Yang, Chao Xiong, Wenzheng Cheng, Aijing Shang, Yaru Liu, Xiaofei Guo, Bowen Zhang, Bo Zhang, Saidi Jin, Wenyi Qi, Berezhnova Tatjana Alexandrovna, Yuan Jiang, Zhimin Du, Chaoqian Xu   +16 more
wiley   +1 more source

Tetramerisation of the CRISPR ring nuclease Crn3/Csx3 facilitates cyclic oligoadenylate cleavage

eLife, 2020
Type III CRISPR systems detect foreign RNA and activate the cyclase domain of the Cas10 subunit, generating cyclic oligoadenylate (cOA) molecules that act as a second messenger to signal infection, activating nucleases that degrade the nucleic acid of ...
Januka S Athukoralage, Stuart McQuarrie, Sabine Grüschow, Shirley Graham, Tracey M Gloster, Malcolm F White   +5 more
doaj   +1 more source