Results 51 to 60 of about 2,102,008 (248)

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

Multi-scale phase separation by explosive percolation with single-chromatin loop resolution

Computational and Structural Biotechnology Journal, 2022
The 2 m-long human DNA is tightly intertwined into the cell nucleus of the size of 10 μm. The DNA packing is explained by folding of chromatin fiber.
Kaustav Sengupta, Michał Denkiewicz, Mateusz Chiliński, Teresa Szczepińska, Ayatullah Faruk Mollah, Sevastianos Korsak, Raissa D'Souza, Yijun Ruan, Dariusz Plewczynski   +8 more
doaj   +1 more source

From Cell‐Derived Vesicles to Hybrid Nanovectors: Biological Membranes as Functional Blueprints for Gene Delivery

Advanced Healthcare Materials, EarlyView.
Biologically derived and hybrid nonviral nanovectors are examined as distinct but convergent design approaches. Integrating synthetic components with biologically functionalized membranes allows efficient interactions with complex cellular environments.
Clara Baldari, Claudia Leone, Gabriella Leccese, Claudia De Stradis, Alessandra Invidia, Concetta Quintarelli, Biagio De Angelis, Franco Locatelli, Giuseppe Gigli, Gabriele Maiorano, Ilaria Elena Palamà   +10 more
wiley   +1 more source

An extrinsic motor directs chromatin loop formation by cohesin

The EMBO Journal
The ring-shaped cohesin complex topologically entraps two DNA molecules to establish sister chromatid cohesion. Cohesin also shapes the interphase chromatin landscape with wide-ranging implications for gene regulation, and cohesin is thought to achieve ...
Thomas M Guérin, Christopher Barrington, Georgii Pobegalov, Maxim I Molodtsov, Frank Uhlmann   +4 more
doaj   +1 more source

Cohesin mediates DNA loop extrusion and sister chromatid cohesion by distinct mechanisms

Molecular Cell, 2022
Summary Cohesin connects CTCF binding sites and other genomic loci in cis to form chromatin loops, and replicated DNA molecules in trans to mediate sister chromatid cohesion.
Kota Nagasaka, Iain F. Davidson, Roman R. Stocsits, Wen Tang, Gordana Wutz, Paul Betty, Melanie Panarotto, Gabriele Litos, Alexander Schleiffer, Daniel Wolfram Gerlich, Jan-Michael Peters   +10 more
openaire   +2 more sources

Targeting CD300a Signaling With a Phosphatidylserine‐Presenting Hydrogel Rescues Macrophage Dysfunction and Accelerates Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Schematic illustration of the proposed mechanism: PEG/RGD‐PSLs mimic apoptotic cells to engage PS receptors (notably CD300a), transducing an inhibitory signal that suppresses the MyD88/NF‐κB pathway, leading to global anti‐inflammatory and pro‐reparative effects.
Lele Wu, Xuetong Hao, Yang Zou, Xinglin Chen, Junyan Kong, Chunning Gu, Wenjie Ma, Zhongjun Yang, Huifen Liu, Mengmeng Liu, Xin Tong   +10 more
wiley   +1 more source

Prediction of CTCF loop anchor based on machine learning

Frontiers in Genetics, 2023
Introduction: Various activities in biological cells are affected by three-dimensional genome structure. The insulators play an important role in the organization of higher-order structure.
Xiao Zhang, Xiao Zhang, Xiao Zhang, Wen Zhu, Wen Zhu, Huimin Sun, Yijie Ding, Li Liu   +7 more
doaj   +1 more source

PLL‐g‐HPA Hydrogel Microneedles Loaded With Rhoifolin Target Macrophages to Alleviate Fibroblast Senescence for Diabetic Wound Healing

Advanced Healthcare Materials, EarlyView.
Diabetic wound healing is hindered by a pathological crosstalk wherein elevated IL‐11 induces M1 macrophage polarization, leading to IL‐1β/IL‐6 release and subsequent fibroblast senescence via RAS/p38MAPK/p53/E2F1 signaling. Here, we develop a Gel@M‐Rho microneedle patch that delivers rhoifolin‐loaded, macrophage membrane‐coated nanoparticles to target
Jiewen Liao, Lin Gan, Li Lu, Lizhi Ouyang, Yuan Xiong, Fawwaz Al‐Smadi, Guandong Dai, Mohammad‐Ali Shahbazi, Bobin Mi, Mengfei Liu, Guohui Liu   +10 more
wiley   +1 more source

Limits of Chromosome Compaction by Loop-Extruding Motors

Physical Review X, 2019
During mitosis, human chromosomes are linearly compacted about 1000-fold by loop-extruding motors. Recent experiments have shown that condensins extrude DNA loops but in a “one-sided” manner.
Edward J. Banigan, Leonid A. Mirny
doaj   +1 more source

DNA hypermethylation/boundary control loss identified in retinoblastomas associated with genetic and epigenetic inactivation of the RB1 gene promoter

Epigenetics, 2021
DNA hypermethylation events occur frequently in human cancers, but less is known of the mechanisms leading to their initiation. Retinoblastoma, an intraocular cancer affecting young children, involves bi-allelic inactivation of the RB1 gene (RB−/-).
AM Raizis, HM Racher, A Foucal, H Dimaras, BL Gallie, PM George   +5 more
doaj   +1 more source