Results 61 to 70 of about 2,219,993 (156)

The SMC Hinge is a Selective Gate for Obstacle Bypass

Nature Communications
DNA loop-extruding SMC complexes play vital roles in genome maintenance and DNA immunity. However, how these ring-shaped DNA motors navigate large DNA-bound obstacles has remained unclear.
Hon Wing Liu, Florian Roisné-Hamelin, Michael Taschner, James Collier, Madhusudhan Srinivasan, Stephan Gruber   +5 more
doaj   +1 more source

Cohesin distribution alone predicts chromatin organization in yeast via conserved-current loop extrusion

Genome Biology
Background Inhomogeneous patterns of chromatin-chromatin contacts within 10–100-kb-sized regions of the genome are a generic feature of chromatin spatial organization.
Tianyu Yuan, Hao Yan, Kevin C. Li, Ivan Surovtsev, Megan C. King, Simon G. J. Mochrie   +5 more
doaj   +1 more source

Loop-extruding Smc5/6 organizes transcription-induced positive DNA supercoils [PDF]

The structural maintenance of chromosomes (SMC) protein complexes-cohesin, condensin, and the Smc5/6 complex (Smc5/6)-are essential for chromosome function. At the molecular level, these complexes fold DNA by loop extrusion.
Kim, E. ; https://orcid.org/, Sutani, T., Berta, D., Umeda Igarashi, M., Kanno, T., Jeppsson, K., Björkegren, C., Sakata, T., Pradhan, B. ; https://orcid.org/, Shirahige, K., Nakato, R., Sutani, T, Sakata, T,, Jeppsson, K,, Berta, DG, Pradhan, B, Igarashi, MU, Shirahige, K,, Kanno, T,, Kim, E, Nakato, R   +20 more
core   +1 more source

STAG2-RAD21 complex: A unidirectional DNA ratchet mechanism in loop extrusion

International Journal of Biological Macromolecules
DNA loop extrusion plays a key role in the regulation of gene expression and the structural arrangement of chromatin. Most existing mechanistic models of loop extrusion depend on some type of ratchet mechanism, which should permit the elongation of loops while preventing their collapse, by enabling DNA to move in only one direction.
David Ros-Pardo, Paulino Gómez-Puertas, Íñigo Marcos-Alcalde   +2 more
openaire   +3 more sources

High-resolution CTCF footprinting reveals impact of chromatin state on cohesin extrusion

Nature Communications
Cohesin-mediated DNA loop extrusion enables gene regulation by distal enhancers through the establishment of chromosome structure and long-range enhancer-promoter interactions.
Corriene E. Sept, Y. Esther Tak, Viraat Goel, Mital S. Bhakta, Christian G. Cerda-Smith, Haley M. Hutchinson, Marco Blanchette, Christine E. Eyler, Sarah E. Johnstone, J. Keith Joung, Anders S. Hansen, Martin J. Aryee   +11 more
doaj   +1 more source

Cohesin mutations alter DNA damage repair and chromatin structure and create therapeutic vulnerabilities in MDS/AML

JCI Insight, 2021
The cohesin complex plays an essential role in chromosome maintenance and transcriptional regulation. Recurrent somatic mutations in the cohesin complex are frequent genetic drivers in cancer, including myelodysplastic syndromes (MDS) and acute myeloid ...
Zuzana Tothova, Anne-Laure Valton, Rebecca A. Gorelov, Mounica Vallurupalli, John M. Krill-Burger, Amie Holmes, Catherine C. Landers, J. Erika Haydu, Edyta Malolepsza, Christina Hartigan, Melanie Donahue, Katerina D. Popova, Sebastian Koochaki, Sergey V. Venev, Jeanne Rivera, Edwin Chen, Kasper Lage, Monica Schenone, Alan D. D’Andrea, Steven A. Carr, Elizabeth A. Morgan, Job Dekker, Benjamin L. Ebert   +22 more
doaj   +1 more source

Diffusion of activated ATM explains γH2AX and MDC1 spread beyond the DNA damage site

iScience
Summary: During DNA repair, ATM-induced H2AX histone phosphorylation and MDC1 recruitment spread megabases beyond the damage site. While loop extrusion has been suggested to drive this spread, the underlying mechanism remains unclear.
Georgi Danovski, Greta Panova, Bradley Keister, Georgi Georgiev, Aleksandar Atemin, Sonya Uzunova, Rumen Stamatov, Petar-Bogomil Kanev, Radoslav Aleksandrov, Krastan B. Blagoev, Stoyno S. Stoynov   +10 more
doaj   +1 more source

Computational prediction of CTCF/cohesin-based intra-TAD loops that insulate chromatin contacts and gene expression in mouse liver

eLife, 2018
CTCF and cohesin are key drivers of 3D-nuclear organization, anchoring the megabase-scale Topologically Associating Domains (TADs) that segment the genome.
Bryan J Matthews, David J Waxman
doaj   +1 more source

Symmetry of loop extrusion by dimeric SMC complexes is DNA-tension-dependent

Structural maintenance of chromosome (SMC) complexes organize and regulate genomes via DNA loop extrusion. During this process, the complexes increase the loop size by reeling in DNA from one or both sides of the loop. The factors governing this symmetry
Chatzicharlampous, Constantinos, Pradhan, Biswajit, Schüler, Herwig, Aragon, Luis, Pinto, Adrian, Corbett, Kevin D, Tetiker, Damla, Kanno, Takaharu, Cutt, Erin, Deep, Amar, Kim, Eugene, Baaske, Martin D., Virnau, Peter, Björkegren, Camilla, Pradhan, Biswajit,, Chatzicharlampous, Constantinos,, Deep, A, Virnau, Peter,, Pinto, A, Kanno, Takaharu,, Cutt, Erin,, Pinto, Adrian,, Tetiker, D, Corbett, Kevin D,, Schüler, Herwig,, Baaske, MD, Baaske, Martin D.,, Corbett, KD, Kanno, T, Schüler, H, Björkegren, Camilla,, Pradhan, B, Kim, Eugene,, Chatzicharlampous, C, Aragon, Luis,, Lund University., Tetiker, Damla,, Cutt, E, Deep, Amar,   +38 more
core   +1 more source

RNAP II antagonizes mitotic chromatin folding and chromosome segregation by condensin

Cell Reports
Summary: Condensin shapes mitotic chromosomes by folding chromatin into loops, but whether it does so by DNA-loop extrusion remains speculative. Although loop-extruding cohesin is stalled by transcription, the impact of transcription on condensin, which ...
Jérémy Lebreton, Léonard Colin, Elodie Chatre, Pascal Bernard   +3 more
doaj   +1 more source