Results 81 to 90 of about 2,219,993 (156)

Loop-extruders alter bacterial chromosome topology to direct entropic forces for segregation

Nature Communications
Entropic forces have been argued to drive bacterial chromosome segregation during replication. In many bacterial species, however, specifically evolved mechanisms, such as loop-extruding SMC complexes and the ParABS origin segregation system, contribute ...
Janni Harju, Muriel C. F. van Teeseling, Chase P. Broedersz   +2 more
doaj   +1 more source

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ

Nature Communications
The spatial organization of the genome is essential for its functions, including gene expression and chromosome segregation. Phase separation and loop extrusion have been proposed to underlie compartments and topologically associating domains, however ...
K. S. Beckwith, Ø. Ødegård-Fougner, N. R. Morero, C. Barton, F. Schueder, W. Tang, S. Alexander, J- M. Peters, R. Jungmann, E. Birney, J. Ellenberg   +10 more
doaj   +1 more source

Real-time detection of cruciform extrusion by single-molecule DNA nanomanipulation.

, 2011
International audienceDuring cruciform extrusion, a DNA inverted repeat unwinds and forms a four-way junction in which two of the branches consist of hairpin structures obtained by self-pairing of the inverted repeats.
R. Sachidanandam, Ramreddy, T., T. Ramreddy, Sachidanandam, R., Strick, Terence, T. R. Strick   +5 more
core   +1 more source

MITOCHONDRIAL DNA POLYMORPHISMS AND FERTILITY IN BEEF CATTLE [PDF]

, 2002
Two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 422 beef cattle of Hereford and composite breeds from Wokalup’s ...
A.J, Lymbery, J.M, Cummins, ., Sutarno, RCA, Thompson   +3 more
core  

DCAF15 control of cohesin dynamics sustains acute myeloid leukemia

Nature Communications
The CRL4-DCAF15 E3 ubiquitin ligase complex is targeted by the aryl-sulfonamide molecular glues, leading to neo-substrate recruitment, ubiquitination, and proteasomal degradation. However, the physiological function of DCAF15 remains unknown.
Grant P. Grothusen, Renxu Chang, Zhendong Cao, Nan Zhou, Monika Mittal, Arindam Datta, Phillip Wulfridge, Thomas Beer, Baiyun Wang, Ning Zheng, Hsin-Yao Tang, Kavitha Sarma, Roger A. Greenberg, Junwei Shi, Luca Busino   +14 more
doaj   +1 more source

Turning the Mre11/Rad50 DNA repair complex on its head: lessons from SMC protein hinges, dynamic coiled-coil movements and DNA loop-extrusion? [PDF]

Biochem Soc Trans, 2020
Zabolotnaya E, Mela I, Henderson RM, Robinson NP.   +3 more
europepmc   +1 more source

Replisomes restrict SMC translocation in vivo

Nature Communications
Structural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are ...
Qin Liao, Hugo B. Brandão, Zhongqing Ren, Xindan Wang   +3 more
doaj   +1 more source

Characterization of induced cohesin loop extrusion trajectories in living cells

Cohesin (SMC1–SMC3–RAD21) constantly extrudes DNA loops to organize chromosomes into structural domains, pausing and anchoring at specific DNA-bound CTCF molecules.
Vaandrager, Iwan, de Wit, Elzo, Allahyar, Amin, CMM Groep De Ridder, de Laat, Wouter, Han, Ruiqi, Genetica, Zhang, Kexin, Krijger, Peter H.L., Magnitov, Mikhail, Verstegen, Marjon J.A.M., Genetica Sectie Research, Huang, Yike, Robers, Michelle J., Hubrecht Institute with UMC, Cancer   +15 more
core  

An upstream secondary DNA motif within the IL3 insulator CTCF binding site is required for enhancer-blocking insulator activity.

PLoS ONE
CCCTC-binding Factor (CTCF) is the only known vertebrate protein that functions to organise the genome into distinct functional chromatin domains. A subset of CTCF sites also provide insulator activity and barrier activity to block inappropriate enhancer-
Sarion R Bowers, Peter N Cockerill
doaj   +1 more source

Closed-Loop Control of Extrusion-Based Bioprinting Through Real-Time Computer Vision

Bioprinting is the technology that combines the use of living matter and biomaterials to manufacture biological models, tissues, and structures layer by layer for applications in regenerative medicine, drug testing, and tissue engineering.
Javier Arduengo, Nicolas Hascoët, Jean-Yves Hascoet, Francisco Chinesta   +3 more
core   +1 more source